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Offlinemroy1300
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Registered: 04/29/11
Posts: 48
Last seen: 12 years, 8 months
noob contamination question
    #14371110 - 04/29/11 03:44 AM (12 years, 10 months ago)

Hello all I have been reading posts here for awhile and I love the site. Amazing, any way I recently decided to try mushroom cultivation I love how interesting it is and the speed that is grows is a defiant plus.

On to the post...

So about 14 months ago I purchased a spore syringe from spores101 since then I have read a lot of negative things about them but I figured at the very least this would be a learning experience.

I inoculated 6 jars on 4/19/11 using the PF-tek method as I'm sure you can see from the post this was 10 days ago exactly, I inoculated with a glove box and used lots of Lysol and rubbing alcohol.

I keep the jars in a sterilite tub with the lid on (I should point out I am very paranoid about contamination however I DID NOT flame the needle in between jars only wiped it with RA.)

I could only find fine grade vermiculite so I am not sure if what I am seeing is contamination or simply just the specs of verm showing through.

Seeing that I got my spores from spores101 I would love some opinions on my jars, The temperature ranges from 65 degrees Fahrenheit at night to 85 degrees Fahrenheit in mid-day with a humidity level of about 45% the jars themselves have a lot of clear moisture visible (I'm not sure if you can see it in the pictures.)

I hope my first grow is in good shape I'm trying to learn about casings and liquid culture to speed up my colonization and hopefully produce more fruit in the end.

Another question I had is what humidity level should you fruit for the B+ strain? I have found cases where humidity is to high they will just continue to grow mycelium. I am planning on following the PF-tek with a smaller terrarium and just the soaked perlite. I suppose if anything I am more worried about not having enough humidity due to the holes in all six sides as well as contaminates finding a nice home in the soaking wet perlite, I was thinking it might be wise to put a 10:1 solution of h2o2 in with the water I uses to soak the perlite?

Opinions??

Also what is the best way to preserve your mycelium and how long will a liquid culture be good for?

I am sorry about all the questions but you don't know if you don't ask and it would save a lot of experimental time. I figure if I am going to do it I might as well do it right.

Just a quick note: In the first three pictures the flash is not on so the mycelium may look a little darker in color but I made sure to get a few with the flash as well.

I think I uploaded the pictures correctly. Let me know if anyone has any problems viewing them. Thank you.



--------------------
Give a man a colonized substrate he grows a flush or two, Teach a man to colonize a substrate and he fills the world with fungus. (It's a good thing.)

Happy growing and good luck.

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Offlinedizzyease
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Registered: 07/27/10
Posts: 541
Loc: Canada Flag
Last seen: 10 years, 11 months
Re: noob contamination question [Re: mroy1300]
    #14373820 - 04/29/11 05:27 PM (12 years, 10 months ago)

well, everything looks cool so far dude!
myc looks nice, your fine verm doesnt seem to change the substrate too much.
I have had an LC sitting at room temp, (50 F - 80 F) for about 6 months, and its chugging along, so obviously its pretty resilient. LCs are the best way to preserve them, or maybe perhaps a grain culture? I just use spore prints or syringes to store spores, not myc. dont get me wrong, i have two lcs kicking around for fun, but they're not my main thing, i use spores more often.

dunno about your humidity question, im fruiting at 70 ish RH which isnt recommended, i just suck at FCs, but they always fruit eventually.

anything else? :smile:

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Offlinemroy1300
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Registered: 04/29/11
Posts: 48
Last seen: 12 years, 8 months
Re: noob contamination question [Re: dizzyease]
    #14373935 - 04/29/11 05:53 PM (12 years, 10 months ago)

Wow thank you for that amazing post. I wasn't expecting any replies not sure why but you have helped put my worries at bay lol, at least for now. Thanks again


--------------------
Give a man a colonized substrate he grows a flush or two, Teach a man to colonize a substrate and he fills the world with fungus. (It's a good thing.)

Happy growing and good luck.

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Offlinedizzyease
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Registered: 07/27/10
Posts: 541
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Last seen: 10 years, 11 months
Re: noob contamination question [Re: mroy1300]
    #14374229 - 04/29/11 07:14 PM (12 years, 10 months ago)

lol, no problemo man, its no doubt an exciting hobby.
i actually have the same size jars, with golden teachers, pinning right now.
after colonization to 100 %, i waited a week, and birthed. The day i birthed, was exactly a week ago. Today, to my surprise, two little pins, in my fucked up, totally wrong, 70 % RH, 68 F  so called shotgun terrarium, so things are looking good for me :smile:

if i can get away with this much, your gonna do great!
p.s b+ is a fun strain, you will probably have no trouble, :smile:

EDIT: Btw, your jars are upside down, turn them right side up as they colonize, then when they have the bottom to finish, turn them over, smack them, and set them down. dont worry about contams too much by then, if you turn your jars now they will most likely colonize faster! :smile:

Edited by dizzyease (04/29/11 07:15 PM)

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Offlinemroy1300
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Registered: 04/29/11
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Last seen: 12 years, 8 months
Re: noob contamination question [Re: dizzyease]
    #14374801 - 04/29/11 09:18 PM (12 years, 10 months ago)

Quote:

dizzyease said:
Btw, your jars are upside down, turn them right side up as they colonize, then when they have the bottom to finish, turn them over, smack them, and set them down. dont worry about contams too much by then, if you turn your jars now they will most likely colonize faster! :smile:




When you say "smack them, and set them down." you don't mean to birth them right? I would assume you mean not to birth them but just separate the cake from the interior of the jar with that slamming motion, upside down, so that the bottoms can colonize in the small gap now formed. Correct?

As I understand it you want to wait 1 week after 100% colonization before birthing?

I'm glad to hear yours are going well, Out of curiosity how much are you doing? also what do you expect you will get from that? How many flushes are you expecting to get? I hear that 2 is average (I'm not sure about with the golden teachers thou.) but you can get 3 if conditions are right. Just what I have read.

When I purchased my spores I actually had the golden teachers in my shopping cart debating on what one to get lol. I figured the B+ was PFtek proven, and perfect for BRF cakes. So a safe bet in my book.

I love BRF cakes they make the whole first 2 weeks to a month easy as pie. And after birthing its just as easy you just gotta make sure they look pretty everyday, weather you gotta fan, mist, heat, air, o2, whatever.

I did not worry a lot when I inoculated regretfully, I was to excited lol. Didn't flame between jars, Room I used my glove box in was not the most sterile room, but it wasn't in the kitchen or the bathroom. I hear thats the 2 worst places to inoculate.

I'm sorry the post is so long I'm really developing a passion for this.:smile: I may have found my calling lol.

So far so good. Fingers Crossed!


--------------------
Give a man a colonized substrate he grows a flush or two, Teach a man to colonize a substrate and he fills the world with fungus. (It's a good thing.)

Happy growing and good luck.

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Offlinedizzyease
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Registered: 07/27/10
Posts: 541
Loc: Canada Flag
Last seen: 10 years, 11 months
Re: noob contamination question [Re: mroy1300]
    #14374956 - 04/29/11 09:51 PM (12 years, 10 months ago)

Quote:

mroy1300 said:
Quote:

dizzyease said:
Btw, your jars are upside down, turn them right side up as they colonize, then when they have the bottom to finish, turn them over, smack them, and set them down. dont worry about contams too much by then, if you turn your jars now they will most likely colonize faster! :smile:




When you say "smack them, and set them down." you don't mean to birth them right? I would assume you mean not to birth them but just separate the cake from the interior of the jar with that slamming motion, upside down, so that the bottoms can colonize in the small gap now formed. Correct?

As I understand it you want to wait 1 week after 100% colonization before birthing?

I'm glad to hear yours are going well, Out of curiosity how much are you doing? also what do you expect you will get from that? How many flushes are you expecting to get? I hear that 2 is average (I'm not sure about with the golden teachers thou.) but you can get 3 if conditions are right. Just what I have read.

When I purchased my spores I actually had the golden teachers in my shopping cart debating on what one to get lol. I figured the B+ was PFtek proven, and perfect for BRF cakes. So a safe bet in my book.

I love BRF cakes they make the whole first 2 weeks to a month easy as pie. And after birthing its just as easy you just gotta make sure they look pretty everyday, weather you gotta fan, mist, heat, air, o2, whatever.

I did not worry a lot when I inoculated regretfully, I was to excited lol. Didn't flame between jars, Room I used my glove box in was not the most sterile room, but it wasn't in the kitchen or the bathroom. I hear thats the 2 worst places to inoculate.

I'm sorry the post is so long I'm really developing a passion for this.:smile: I may have found my calling lol.

So far so good. Fingers Crossed!




nope, dont birth, definately not a good idea, haha.
but yes, smacking them down makes them suction off the glass in the jar, and in turn it sucks more air in, but you do that after about 80 % colonize, like if only the bottom of the jar is done. you dont HAVE to do this, unless it stalls. but it definately speeds it up a bit.

and yes, after you see 100 % myc, and no substrate, you need to wait a week, for consolidation, this is when the myc finishes growing not only outside the cake, but in the core too. this also gives it time to organize itself, and start to digest the food.

after 7 days, it will be done, and will be about a week to see pins. then you deal with your pinning schedule, with the triggers and such.

i'm doing 36 jars of golden teachers, not much, but personal stuff.

well, average per cake is 4 - 6 grams, less commonly 3 - 8
im doing 36 jars, so minimum, i should have 144 grams, or 5 ounces.
i dunno if that includes all flushes, or per flush.
anyways, i most likely get three big flushes and a small flush at the end. by then i keep waiting to get everything i can from them.

i actually did 12 jars, didnt flame in between them because i just got caught ahead of myself. they turned out fine, and i steamed my jars for an hour and a half, not pressure cooked :P

its not THAT big of a deal, but dont skip it if you can remember, it can save your crop from getting ALL contaminated, or only one or two jars. :P

if you used a glovebox, you really will be fine, especially for only six jars. when you have 36, and are putting them in a glovebox in cycles, then it gets a little more risky. obviously not much though, seeing im still 100% contam free.

and dont worry about it man, learning is learning :smile:

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Offlinemroy1300
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Registered: 04/29/11
Posts: 48
Last seen: 12 years, 8 months
Re: noob contamination question [Re: dizzyease]
    #14375058 - 04/29/11 10:14 PM (12 years, 10 months ago)

Thank you for the wealth of information you are very through.

It nice to interact with someone who is actually genuine on here. Seems like everyone else is either to busy to be through, or there so set in there ways they wont accept ideas, or there just plain jerks about everything.

Its nice to see there are a select few people here who will take a second to explain something just to make sure a lesser experienced person does it right? I feel like everyone else answers very vaguely then wont respond to clarify.

So one more time. Thank you For answering my lame ass noob questions lol.


--------------------
Give a man a colonized substrate he grows a flush or two, Teach a man to colonize a substrate and he fills the world with fungus. (It's a good thing.)

Happy growing and good luck.

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Offlinemroy1300
Super cool guy
Male


Registered: 04/29/11
Posts: 48
Last seen: 12 years, 8 months
Re: noob contamination question [Re: dizzyease]
    #14375110 - 04/29/11 10:24 PM (12 years, 10 months ago)

Not to be a bother but you were such a great help with my last thread maybe you could shed some light on a different thread I started.

http://www.shroomery.org/forums/showflat.php/Number/14374464

I you have a spare moment give it a quick read.

Thank you.


--------------------
Give a man a colonized substrate he grows a flush or two, Teach a man to colonize a substrate and he fills the world with fungus. (It's a good thing.)

Happy growing and good luck.

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Offlinedizzyease
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Registered: 07/27/10
Posts: 541
Loc: Canada Flag
Last seen: 10 years, 11 months
Re: noob contamination question [Re: mroy1300]
    #14375144 - 04/29/11 10:33 PM (12 years, 10 months ago)

yeah, its a busy place,
theres a lot of smart people on there though, just need to explain more, lmao
I dont mind, its cool to see new growers get into this kind of stuff! your doing well so far, so i'd expect some nice results :smile:

keep us updated, hope to see you around! :smile: :thumbup:

and sure, ill take a look at it :smile:

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Offlinemroy1300
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Male


Registered: 04/29/11
Posts: 48
Last seen: 12 years, 8 months
Re: noob contamination question [Re: dizzyease]
    #14375243 - 04/29/11 10:54 PM (12 years, 10 months ago)

Thank you I will defiantly keep everyone posted.

I cant wait to see how it goes even if I do get an infection at some point then I will just know to do it better next time. I have been reading up on the rules to determining what caused contamination. That way I will be able to determine exactly where I went wrong and perfect my method and slowly but surely I will be ahead of my game.

This is defiantly the #1 mushroom site for information and feedback.

Thank you for all the support.


--------------------
Give a man a colonized substrate he grows a flush or two, Teach a man to colonize a substrate and he fills the world with fungus. (It's a good thing.)

Happy growing and good luck.

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OfflineJohann
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Registered: 04/19/11
Posts: 5
Last seen: 12 years, 6 months
Re: noob contamination question [Re: mroy1300]
    #14760982 - 07/13/11 05:39 AM (12 years, 7 months ago)

i got a wet spot in my mycelium but it looks like its the mycelium thats wet not the jar on the inside ill try to add a pic as well

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