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Marfa
Stranger

Registered: 04/25/11
Posts: 11
Loc: Argentina, Buenos Aires
Last seen: 7 years, 10 months
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Help on cloning
#14372733 - 04/29/11 01:58 PM (12 years, 8 months ago) |
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Hello, I am pretty knew to this site but I have been lurking around for information and tips to grow mushrooms. I would like to know if you could give me some advice. First, I come from Argentina and where I live, it is extremely hard to find fresh oyster mushrooms, so I can just try and clone a dry one with RogerRabbit´s method to rehydrate the dry mushroom chunks. I have seen it work the first time I tried it but when I put the chunks into some Potatoe agar mix with peroxide, the mycelium would just grow off the tissue but not over the agar, I did notice that the chunks I used were pretty big, about half a grape size. Why is that happening? Second, as I can get no fresh oysters, I have tried to clone some Agaricus Bisporus tissue, but the mycelium wouldn´t even grow off the chunk. I could only notice contamination and no mycelium. Is it my luck, the formula I am using or is the Agaricus´mycelium too similar to contaminants. Finally, I would really appreciate if you could tell me the best way to grow oysters or button mushrooms knowing that I don´t have great equipment such as a pressure cooker, and not having to spend too much money for it.
Thank you for your time.
Marfa.
-------------------- "Only when the last tree has been cut down; Only when the last river has been poisoned; Only when the last fish has been caught; Only then will you find that money cannot be eaten."
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
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Re: Help on cloning [Re: Marfa]
#14372781 - 04/29/11 02:07 PM (12 years, 8 months ago) |
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could be the peroxide... I'm pretty sure that is not supposed to be in an agar recipe.
get yourself a copy of Stamets Growing Gourmet and Medicinal Mushrooms or keep reading on shroomery and other mushy-sites. All the info is out there, but no one is going to give you step-by-step specific answers to every question you can come up with.
once you have a solid information foundation, and some money to get the proper equipment, jump right into it and start making mistakes! 
afterward, you'll know what not to do and start seeing some success.
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Marfa
Stranger

Registered: 04/25/11
Posts: 11
Loc: Argentina, Buenos Aires
Last seen: 7 years, 10 months
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thank you for the reply, next time I will try without any peroxide
-------------------- "Only when the last tree has been cut down; Only when the last river has been poisoned; Only when the last fish has been caught; Only then will you find that money cannot be eaten."
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