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OfflineMarfa
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Registered: 04/25/11
Posts: 11
Loc: Argentina, Buenos Aires Flag
Last seen: 7 years, 10 months
Help on cloning
    #14372733 - 04/29/11 01:58 PM (12 years, 8 months ago)

Hello, I am pretty knew to this site but I have been lurking around for information and tips to grow mushrooms. I would like to know if you could give me some advice.
First, I come from Argentina and where I live, it is extremely hard to find fresh oyster mushrooms, so I can just try and clone a dry one with RogerRabbit´s method to rehydrate the dry mushroom chunks. I have seen it work the first time I tried it but when I put the chunks into some Potatoe agar mix with peroxide, the mycelium would just grow off the tissue but not over the agar, I did notice that the chunks I used were pretty big, about half a grape size. Why is that happening?
Second, as I can get no fresh oysters, I have tried to clone some Agaricus Bisporus tissue, but the mycelium wouldn´t even grow off the chunk. I could only notice contamination and no mycelium. Is it my luck, the formula I am using or is the Agaricus´mycelium too similar to contaminants.
Finally, I would really appreciate if you could tell me the best way to grow oysters or button mushrooms knowing that I don´t have great equipment such as a pressure cooker, and not having to spend too much money for it.

Thank you for your time.

                        Marfa.


--------------------
"Only when the last tree has been cut down;
Only when the last river has been poisoned;
Only when the last fish has been caught;
Only then will you find that money cannot be eaten."


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OfflinePrimal Call
Earth Mage
I'm a teapot


Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
Re: Help on cloning [Re: Marfa]
    #14372781 - 04/29/11 02:07 PM (12 years, 8 months ago)

could be the peroxide... I'm pretty sure that is not supposed to be in an agar recipe.

get yourself a copy of Stamets Growing Gourmet and Medicinal Mushrooms or keep reading on shroomery and other mushy-sites. All the info is out there, but no one is going to give you step-by-step specific answers to every question you can come up with.

once you have a solid information foundation, and some money to get the proper equipment, jump right into it and start making mistakes! :evil2:

afterward, you'll know what not to do and start seeing some success.

:peace:


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New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


:peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace:


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OfflineMarfa
Stranger
Male User Gallery
Registered: 04/25/11
Posts: 11
Loc: Argentina, Buenos Aires Flag
Last seen: 7 years, 10 months
Re: Help on cloning [Re: Primal Call]
    #14374100 - 04/29/11 06:38 PM (12 years, 8 months ago)

thank you for the reply, next time I will try without any peroxide


--------------------
"Only when the last tree has been cut down;
Only when the last river has been poisoned;
Only when the last fish has been caught;
Only then will you find that money cannot be eaten."


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