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OfflinefungalEntropy
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Registered: 05/17/10
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Agar's Grain LC Tek * 1
    #14365864 - 04/28/11 09:07 AM (12 years, 10 months ago)

Going to be attempting Agars grain lc tek,  How long do I need to PC the jar of water until it is sterile?  Also Should I PC the syringes I'll be using?  or should I just go with the boiling water method then suck up the boiling water 4x?  If I should PC the syringes what's the best way to do so?

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OfflinefungalEntropy
misfiring synapses


Registered: 05/17/10
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Re: Agar's Grain LC Tek [Re: fungalEntropy]
    #14365959 - 04/28/11 09:33 AM (12 years, 10 months ago)

So how long to I need to PC the jar of water to sterilize it?

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InvisibleDoc_T
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Re: Agar's Grain LC Tek [Re: fungalEntropy]
    #14365963 - 04/28/11 09:34 AM (12 years, 10 months ago)

I PC things that I want to sterilize. Boiling does not sterilize water, as any boy scout can tell you.


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Invisibleskyjohnny
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Re: Agar's Grain LC Tek [Re: fungalEntropy]
    #14365974 - 04/28/11 09:36 AM (12 years, 10 months ago)

Water won't take nearly as long as say grain, to reach temp... but I always PC for an hour anyway, just to be sure.


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"Man is that he might have joy. Joy is the "true meaning" of life. May you all experience "true joy". -CJM

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OfflinefungalEntropy
misfiring synapses


Registered: 05/17/10
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Re: Agar's Grain LC Tek [Re: Doc_T]
    #14371925 - 04/29/11 10:17 AM (12 years, 10 months ago)

Quote:

Doc_T said:
I PC things that I want to sterilize. Boiling does not sterilize water, as any boy scout can tell you.




So madhatters "how to sterilize a syringe" doesn't actually sterilize it?  Where you boil water for ten minutes then suck boiling water in the syringe then spit it out in the sink then repeat 4xtimes then on the last time leave the water in?

Also PC'd three jars with water and three syringes wrapped in tin foil for an hour.

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Invisiblehamloaf
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Registered: 12/23/09
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Re: Agar's Grain LC Tek [Re: fungalEntropy]
    #14371939 - 04/29/11 10:25 AM (12 years, 10 months ago)

Quote:

fungalEntropy said:
Quote:

Doc_T said:
I PC things that I want to sterilize. Boiling does not sterilize water, as any boy scout can tell you.




So madhatters "how to sterilize a syringe" doesn't actually sterilize it?  Where you boil water for ten minutes then suck boiling water in the syringe then spit it out in the sink then repeat 4xtimes then on the last time leave the water in?

Also PC'd three jars with water and three syringes wrapped in tin foil for an hour.



Try using Shea25's method of "sterilizing" syringes (Search user, links in his sig.).  Sterilized 1000's of syringes with succession using Shea's way.


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InvisibleSillicybin
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Re: Agar's Grain LC Tek [Re: fungalEntropy]
    #14372136 - 04/29/11 11:12 AM (12 years, 10 months ago)

Quote:

fungalEntropy said:
So madhatters "how to sterilize a syringe" doesn't actually sterilize it?  Where you boil water for ten minutes then suck boiling water in the syringe then spit it out in the sink then repeat 4xtimes then on the last time leave the water in?




Correct.  It will work a lot of times because tap water also (almost always) has chlorine in it and is usually pretty clean.  It is definitely not anywhere near 100% sterile, though.  There are plenty of spores that can survive being boiled on a stove in an open pot, especially for short durations.

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Offlinek00laid
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Re: Agar's Grain LC Tek [Re: Sillicybin]
    #14372171 - 04/29/11 11:23 AM (12 years, 10 months ago)

Quote:

Sillicybin said:
There are plenty of spores that can survive being boiled on a stove in an open pot, especially for short durations.




but the beauty of the boiling water syringe tek is: all of those spores will be forced to the topmost layer of the water due to the LARGE amount of air flowing upwards through the water.

thus stucking a syringe down below the topmost layer will provide a syringe full of 100% sterile water.


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InvisibleFungal growth
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Re: Agar's Grain LC Tek [Re: k00laid]
    #14372233 - 04/29/11 11:39 AM (12 years, 10 months ago)

also, you should boil the water a minimum of 10 minutes.
if you've lived in a city where mains break regularly,
you have delt with 'boil alerts'. any consumable water MUST
be boiled for a minimum of 10 minutes.
i've used shea's tek for 11 years, it was originally part
of the printed pf tek. works great. i've left syringes
unused for months and used them with 0 contams.
maybe its not sterile, but its close enough to work for me
every time.

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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: Agar's Grain LC Tek [Re: k00laid]
    #14372489 - 04/29/11 12:47 PM (12 years, 10 months ago)

Quote:

k00laid said:
but the beauty of the boiling water syringe tek is: all of those spores will be forced to the topmost layer of the water due to the LARGE amount of air flowing upwards through the water.

thus stucking a syringe down below the topmost layer will provide a syringe full of 100% sterile water.




That's something I hadn't considered.  I'm still not buying 100% sterile, but I know that I have made at least 30 spore syringes using this method that have all worked out just fine.

The only other problem I have with it then is the fact that if you don't first PC your syringes before you suck up the boiling water, you can have nasties in the syringe body and needle that will not be killed when you fill/empty the syringe with boiling water.  Though, you might push out enough of them to be successful.

I guess it all boils down to (:rimshot:) exactly how sure of sterility you feel you need to be.  Can't really argue with what works!

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Offlinek00laid
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Re: Agar's Grain LC Tek [Re: Sillicybin]
    #14372588 - 04/29/11 01:14 PM (12 years, 10 months ago)

Quote:

Sillicybin said:
I guess it all boils down to (:rimshot:) exactly how sure of sterility you feel you need to be.  Can't really argue with what works!



/thread <3


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Invisibleabltsandwich
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Re: Agar's Grain LC Tek [Re: k00laid]
    #14372635 - 04/29/11 01:24 PM (12 years, 10 months ago)

20-30 min. @ 15psi seems to work fine.  If it works for LC it should work for plain old water.

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InvisibleDoc_T
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Re: Agar's Grain LC Tek [Re: abltsandwich]
    #14372828 - 04/29/11 02:16 PM (12 years, 10 months ago)

Tap water is pretty clean to begin with, most places.
I'd like to see numbers on boiled water vs water drawn from a running stream from the tap.


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Offlinek00laid
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Re: Agar's Grain LC Tek [Re: Doc_T]
    #14375197 - 04/29/11 10:46 PM (12 years, 10 months ago)

if i wasn't so lazy i'd quote the dude who makes his syringes from his tap. but he said somethin like this

"wtf you talkin bout my faucet is like a liquid flowhood!!"


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OfflineSomeGuy
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Re: Agar's Grain LC Tek [Re: k00laid]
    #14696238 - 06/30/11 10:04 AM (12 years, 8 months ago)

Quote:

k00laid said:
if i wasn't so lazy i'd quote the dude who makes his syringes from his tap. but he said somethin like this

"wtf you talkin bout my faucet is like a liquid flowhood!!"


:lol:that was me, somewhere in here http://www.shroomery.org/forums/showflat.php/Number/12752577/fpart/1/vc/1

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Offlinek00laid
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Re: Agar's Grain LC Tek [Re: SomeGuy]
    #14696299 - 06/30/11 10:23 AM (12 years, 8 months ago)

bahahah

nice


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