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OfflineBrint
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Registered: 02/04/11
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Last seen: 11 years, 10 months
Spotty growth and excess moisture?
    #14366722 - 04/28/11 12:31 PM (12 years, 9 months ago)

Here's what I have after an April 15th innoc of B+ into BRF per pftek:



Growth seems a bit spotty (and possible discoloration?) and there was a bit of excess moisture slightly pooling at the base. I searched the forums and found a tip to flip the jars upside to drain excess liquid and Co2 into the dry verm layer which I did about two days. Definitely helped with the moisture but the spots don't seem to be filling in during the last week or so. Right after innoc, all 6 cakes went from very subtle signs of mycelium growth at 5 days to almost nearly colonized a few days later. No offensive smells from anything but the rusty-colored speckles with no myce is making me nervous.

Am I just being impatient? Any advice from more experienced mycologists? Thanks friends. :grin:


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Offlinek00laid
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Re: Spotty growth and excess moisture? [Re: Brint]
    #14366731 - 04/28/11 12:32 PM (12 years, 9 months ago)

some bacteria issues it looks like.

see if they ever end up fully colonizing :shrug:


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Offlinesteelmonkey
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Re: Spotty growth and excess moisture? [Re: k00laid]
    #14366753 - 04/28/11 12:39 PM (12 years, 9 months ago)

It could be bacteria or it could be there just stalled I'd open them up and wash all the uncolonized substrate off really good then birth them they look almost fully colonized anyway.

just make sure they have a nice mushroomy smell


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Offlinek00laid
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Re: Spotty growth and excess moisture? [Re: steelmonkey]
    #14366855 - 04/28/11 01:05 PM (12 years, 9 months ago)

defo start more jars if you are counting on these things.


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OfflineBrint
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Re: Spotty growth and excess moisture? [Re: k00laid]
    #14368000 - 04/28/11 04:29 PM (12 years, 9 months ago)

Thanks for the input. I don't want to end up ingesting any bad boys. :confused:
At what point will I know if this is definitely a bacteria issue that cannot be recovered and what did I do wrong that could cause this?


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Offlinek00laid
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Re: Spotty growth and excess moisture? [Re: Brint]
    #14368354 - 04/28/11 05:41 PM (12 years, 9 months ago)

nah that little bacteria will rinse off no prob

if fruits come out they are more than likely safe to eat.

bar them being obviously rotten.


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Offlinemathias
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Re: Spotty growth and excess moisture? [Re: k00laid]
    #14368480 - 04/28/11 06:07 PM (12 years, 9 months ago)

Seems to be a bit too wet in there as well. This can invite contaminates in more easily by making the verm barrier too damp.

Wait until you see pins, or the discoloration worsens and birth as normal. If you are really concerned, a light misting of h2o2 1-10 in water will do the trick. 1-5 if you're really in bad shape. Birth, rinse, mist with the h2o2-mix and let them sit a minute or two. Mist again and rinse off. Dunk in nice clean water.

Then go floss.

Before I got a PC, I saved more than one cake from bacteria this way.

Don't flip if you have verm barriers.

Ever.

That verm is what is keeping out every single mold spore in the environment.
Flipping it (or disturbing it too much in any way) spreads all the contaminates that have collected on the top few microns of the verm barrier.
Not good.

If you've got really good tyvek lids and no verm barrier, ok flip if you want to.
I use tyvek'ed lids, and saw no advantage to flipped jars vs non-flipped. :shrug:

Tone down the water a bit next time, or be sure you're not gaining water in the pc/boil step.
That's a common cause of over-wet jars.

Doo gud foyul jobb.

If the mycelium forms a hard-line edge as opposed to sending fibers into the uncolonized parts, go ahead and birth. This means it is no longer growing outwards, but only firming up what it already has.

Cakes in these circumstances will likely be tough to fruit, so be patient.
Try some direct sun for a few minutes every day, don't mist too much (if anything, go light), fan well but not excessive (another common pitfall) and BE PATIENT!

You made it this far, so props!
You should have good fruits soon!


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intlslacker : "Why is it I always have the feeling that if RR weren't around it'd be like there were toddlers running around, bumping into walls and eating poisonous mushrooms?"


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Offlinemroy1300
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Re: Spotty growth and excess moisture? [Re: mathias]
    #14371224 - 04/29/11 05:14 AM (12 years, 9 months ago)

Don't know if this helps but I have read that it is a good idea to put salt on the uncolonized areas of your cake. I would run this information by someone who has a little more experience in the subject as I have none (I just inoculated my first 6 cakes on 4/19/11 with B+, Wish me luck lol) but it was just a thought.

I would hate for you to overlook anything that would help your situation.

Good luck and props just for trying.


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Give a man a colonized substrate he grows a flush or two, Teach a man to colonize a substrate and he fills the world with fungus. (It's a good thing.)

Happy growing and good luck.


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Offlineluluzuluwoundtdou


Registered: 10/26/10
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Re: Spotty growth and excess moisture? *DELETED* *DELETED* [Re: mroy1300]
    #14371268 - 04/29/11 05:34 AM (12 years, 9 months ago)

Post deleted by rss6612

Reason for deletion: b



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Disclaimer: Im a habitual liar. Anything i say and post is just in my alterior mental reality where i just post up.


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Offlinemathias
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Re: Spotty growth and excess moisture? [Re: luluzuluwoundtdou]
    #14374269 - 04/29/11 07:21 PM (12 years, 8 months ago)

Salt is for dealing with trich, after you cut away the infection. This can work if you catch it VERY early.
Otherwise it's an exercise in futility. Best to avoid trich in the first place...

Un-colonized substrate is to be washed off at birth. Always.


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intlslacker : "Why is it I always have the feeling that if RR weren't around it'd be like there were toddlers running around, bumping into walls and eating poisonous mushrooms?"


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Offlinek00laid
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Re: Spotty growth and excess moisture? [Re: mathias]
    #14375282 - 04/29/11 11:02 PM (12 years, 8 months ago)

salt pastes slow down the trichoderma FEASTING on your mycelium.

they key phrase is slows down.

not much kills trichoderma spores.

90 minutes at 250 Fahrenheit does :laugh:


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OfflineBrint
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Re: Spotty growth and excess moisture? [Re: k00laid]
    #14416172 - 05/07/11 04:52 PM (12 years, 8 months ago)

Thanks for the input everyone. I had to scrap these jars. It was a good learning experience and interesting how difficult the darker contam made it to birth the jars. I have a pressure cooker and I'm following the tek to a T but there must have been excess moisture. I'll post an update on my next experiment and thanks again for the support; great community.


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Offlinemathias
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Re: Spotty growth and excess moisture? [Re: Brint]
    #14419263 - 05/08/11 11:58 AM (12 years, 8 months ago)

Ah, moisture problems. Yup, been there, cried about that.

It's really easy to get soaked jars when boiling. If the water level is too high or you did a less that perfect foil job, *boom*, heavy and wet jars. Half-pint BRF jars should weigh 10-12 ounces when done. Any more or less than that and they are too wet/dry.

Some of the BRF teks I've seen here turn out too wet as well, if you follow them exactly. IMHE, as you're making the mix it should mix up and "feel" moist and fluffy, not sticky and wet. If you get a clump in your hand, it should feel very moist but not dripping wet. Squeeze it and it should keep its shape when you let go, and should not drip any water unless you squeeze really hard. Imagine wet sand when it's perfect for sandcastle building. Or ground beef. Those are the best analogies I have at this point in the space-time continuum.


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intlslacker : "Why is it I always have the feeling that if RR weren't around it'd be like there were toddlers running around, bumping into walls and eating poisonous mushrooms?"


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