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Invisiblefngbronco
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Registered: 09/26/10
Posts: 2,877
Pouring agar over spore print
    #14364404 - 04/27/11 11:19 PM (12 years, 10 months ago)

Just wondering if this would work or even be feasible. If I took a spore print on a petri dish and poured agar into said dish, would there be any huge issues with it not working. I know SS poured agar over some pan spores to invoke them to grow. I'm aware the isolation would he hellish but figure I'll try it unless someone says otherwise.


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I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

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Invisiblewildernessjunkie
Reshitivest
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Re: Pouring agar over spore print [Re: fngbronco]
    #14364476 - 04/27/11 11:30 PM (12 years, 10 months ago)

I want to know the answer to this as well.

Seems like if it will work for one species, it may work for another.

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Offlinejust me
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Re: Pouring agar over spore print [Re: wildernessjunkie]
    #14364533 - 04/27/11 11:42 PM (12 years, 10 months ago)

i think the answer is no

not sure. curious about it as well

im pretty sure tho, that you can "suffocate" spores :lol:

ive sandwiched trichoderma, and lipstick mold btwn two halves of agar and they stalled

as if frozen in time...time being two layers of agar in this case


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Invisiblefngbronco
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Re: Pouring agar over spore print [Re: just me]
    #14364713 - 04/28/11 12:27 AM (12 years, 10 months ago)

I'm thinking the spores can rise with the agar as well if it's broken up. I'll be trying this soon for sure! Just gotta get me a liquor bottle to do agar in and get a spore print into a dish! Will keep this updated for sure.


Edit: I'll try with 2 prints at different temperature agar (by feel), one at comfortable holding temp. The other at "clumpy-ish".


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I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

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Edited by fngbronco (04/28/11 12:33 AM)

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Invisiblemycoelf
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Re: Pouring agar over spore print [Re: fngbronco]
    #14366056 - 04/28/11 10:00 AM (12 years, 10 months ago)

isolation would be hell, and the monocaryots would have a more difficult time finding each other, but in the case of REALLY old spores, I could see it, as a thin layer of agar over the top would eventually rehydrate the overly dried spores, try it and if you do post some pics please, I want to see the resulting jungle :goodluck:


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Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

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Invisiblecc2
Mush

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Re: Pouring agar over spore print [Re: fngbronco]
    #14366886 - 04/28/11 01:12 PM (12 years, 10 months ago)

Quote:

fngbronco said:
If I took a spore print on a petri dish and poured agar into said dish, would there be any huge issues with it not working.




main concern which comes in mind would be burning said spores with molten agar. also you'd be effectively sealing them off any gas including air, which is normally what it takes to successfully germinate them on agar (that's why the grow on top of it and don't dig in it).

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Invisiblefngbronco
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Re: Pouring agar over spore print [Re: cc2]
    #14367767 - 04/28/11 03:53 PM (12 years, 10 months ago)

I didn't consider them being embedded in the hot agar. I'll scrape half the print prior to pouring for sure and I'm gonna pour from the side rather than directly over. Just gotta finish my bottle of brandy and I'll be good to go!


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

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OfflineRogerRabbitM
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Re: Pouring agar over spore print [Re: fngbronco]
    #14367996 - 04/28/11 04:28 PM (12 years, 10 months ago)

You only want a tiny amount of spores so the chances of bacteria and mold spores joining the party are reduced.  I wouldn't pour hot agar over a sporeprint.  Just pour clean dishes and transfer a tiny amount of spores with a loop right to the agar later.
RR


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Invisiblefngbronco
Monkey Man
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Registered: 09/26/10
Posts: 2,877
Re: Pouring agar over spore print [Re: RogerRabbit]
    #14368122 - 04/28/11 04:53 PM (12 years, 10 months ago)

I've tried hydrating spores and dripping them onto agar and smears and I've had 0 luck (although luck isn't what is needed). I've gone with less nutrient agar, less agar, a couple drops of sterile water (in jars w/agar) and still notta. If anything it'll be more of a "I have an extra print let's see what happens" type deal. It's gonna be a super thin layer in hopes it cools quickly and doesn't burst many spores.


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

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InvisibleHacendado
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Re: Pouring agar over spore print [Re: fngbronco]
    #14462058 - 05/16/11 01:13 PM (12 years, 9 months ago)

Any news on this one?

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Invisiblefngbronco
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Registered: 09/26/10
Posts: 2,877
Re: Pouring agar over spore print [Re: Hacendado]
    #14462210 - 05/16/11 01:49 PM (12 years, 9 months ago)

Poured the agar and nothing yet. The good news is however that my agar is CLEAN!!! I may scrape the again that's over the print in order to move it to the top and see if the spores survived the pour.


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

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Invisiblefngbronco
Monkey Man
Male


Registered: 09/26/10
Posts: 2,877
Re: Pouring agar over spore print [Re: fngbronco]
    #14512864 - 05/25/11 11:39 PM (12 years, 9 months ago)

I've got growth! Bacterial, and mold, but only in small colonies, which sadly, is great for me!! I also have some white fluffy right over a print, but we'll see what it turns out to be. I scraped half the print with an inoculation loop, but I don't think they broke free enough. The one that's showing growth has maybe 1mm of agar over it (orange agar is clear in this spot). I poured it hot (burnt my hand after two dishes) but if the spores can survive that temp then they did! Will update when I determine if it's green or myc, got my fingers crossed I get to grow shiitake from ms and work on isolating!


Didn't mess with them after the pour, but the thin one seems to be working. This would also be my first spore germination on agar.


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

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