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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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How 13shrooms does his mono tubs. 16
#14364036 - 04/27/11 10:20 PM (12 years, 8 months ago) |
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I do this for every mono Im preparing. 
First I take a (spores, agar wedges, LC, LI or slurries) and inoculate a qt jar of rye berries that I prepped using this method.
1 Qt Jar of grain for every spawn bag
1 grain spawn bag for every mono tub
*Note - All temperatures used in this procedure are room temps (72-77*F). 
Then once the Qt(s) of grain is/are done colonizing I make a GLC like so 
Quote:
agar said:
 Pint jar on right is multispore inoculated (SA). Allowed to colonize near 100%. Syringe is 60 ML w/18 gauge needle. Quart jar on left is sterilized water.
 Aspirated 60 ML sterile water into syringe.
 Injected 60 ML sterile water into colonized grain jar.
 Shook grain jar to liberate mycelium from grain.
 Injected total of 180 ML sterile water into grain jar. (shaking mildly between each injection)
 Aspirated out mycelium laden water from grain jar. (all I could aspirate out of the grain jar was 150 ML) Then, injected mycelium laden water back into sterile water jar.  Approximately 500 ML of Mycelium laden water.  (enough LC for approximately 50....10 ML syringes)
The advantage of this procedure is that you can be assured. The culture in clean. Because you can VISUALLY inspect colonization in the grain jar. (if there is FUNK, you can see it )
That is not the case, with sugar type LC's. (multispore syringe inoculated) They can be contaminated, from day 1. And, you NEVER know it.  Until you inoculate spawn jars with that LC. Then, have every single spawn jar GO SOUTH. 
* Agar for that write up*
Then once I have myc s ready I store them in my fridge for future use.  (if Im stocked up on myc syringes I skip this step). 
Then I let the rye grain jar re-colonize

and use the whole jar to G2G inoculate 1 large filter patch bag (for each monotub I want to make as I stated b4) in my glovebox/still air box and let that/them fully colonize.


Once the spawn bag(s) is/are finished colonizing I take my monotub(s) (prepped like Ohmatics mono tubs and using Damion5050s Coir tek) and dump all my pre-bucket pasteurized coir/verm substrate and my 1 fully colonized lrg grain spawn bag into my tub. 
then (with gloved hands) mix it very well and level the substrate out to make the surface even/the same for fruiting.  If the sub is uneven and has hills/valleys then you will have pockets of lower/higher humidity in random areas giving you a sparse or spotty pinset. 
To prep my tub for colonization, I put the lid on (most lids dont seal tight so this is where I get my Gas Exchange from during colonization) and tape my tub holes shut w/duct tape until its fully colonized, usually 7-10 days. 

Once 100% fully colonized (surface is all white and covered in myc) I remove the duct tape from the FAE holes and stuff them with polyfill and put into fruiting conditions.

Then I try to keep a constant rotation going. 
  And yes I have a ghetto light set up but each tub is getting its own cfl (6500k spectrum/color) lamp using the 12/12 circadian rhythm light cycle. 
I dont mist or fan (unless the substrate looks dry) during the 1st flush, I dont mess with any of my tubs until its time to harvest 1st flush 
   
Then after all the harvesting is done, I dunk my tub(s) 1 at a time in my bathtub (or whatever you got), I put something kinda heavy (but not too heavy to damage the sub) on top of the substrate to hold it down under water in my mono in my tub. 
4hrs go by and I carefully tip the tub on end (while using my gloved fingertips to hold the sub in the tub) and drain the excess water out.
or use this monotub dunking tek by total
Then I case the substrate using "jiffy mix - seed starter" or 50/50+ casing material.
2qts of field capacity casing is enough mix to cover the surface of 1 mono in about a 1/4" of casing material. 
Then immediately back into fruiting.
I mist/fan 2x a day if needed until the 2nd flush is in.
 
  
I dont go past 2nd flush (because around 85-90% of your total fruits will come in the 1st 2 flushes unless you have other issues) to make room for the next cycle of tubs. 
Feel free to see how many flushes you can do, I just dont worry about the fruits that could be my 3rd flush etc... because I toss all my spent substrates into a pile in my yard and it produces some nice treats every now and then throughout the warmer months of the year. 

Enjoy! 
--------------------
A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: How 13shrooms does his mono tubs. [Re: 13shrooms] 2
#14364074 - 04/27/11 10:25 PM (12 years, 8 months ago) |
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Beautiful write-up 13!
Very clean and comprehensive.
Take care,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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shroomybgood
Almost Novice



Registered: 04/14/11
Posts: 147
Loc: Stuck in my HEAD
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Re: How 13shrooms does his mono tubs. [Re: Javadog] 2
#14364146 - 04/27/11 10:36 PM (12 years, 8 months ago) |
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I will definitely be using some of this! Thanks for the write up!
-------------------- "GET IT TOGETHER ONE MORE TIME"
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Luger0815
noob


Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: How 13shrooms does his mono tubs. [Re: Javadog] 2
#14364163 - 04/27/11 10:39 PM (12 years, 8 months ago) |
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Edited by Luger0815 (04/27/11 10:46 PM)
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afrosheen
9Lives the cat



Registered: 03/06/10
Posts: 1,878
Last seen: 2 years, 3 months
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Re: How 13shrooms does his mono tubs. [Re: Javadog] 2
#14364169 - 04/27/11 10:40 PM (12 years, 8 months ago) |
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Great write up, 5 for you!
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MOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 4 months
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Re: How 13shrooms does his mono tubs. [Re: Javadog] 2
#14364171 - 04/27/11 10:41 PM (12 years, 8 months ago) |
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nice write up, i use the exact same process without the spawn bags, which i would really like to start getting into
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Got used to the feeling of falling But you'll never see me following Bouncing back and forth between the healing and the hollering Riding the outer ring of my own private saturn Thoughts scattered all across the grey matter
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Masticore
Perpetually Curious



Registered: 12/16/09
Posts: 1,159
Last seen: 6 years, 9 months
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Re: How 13shrooms does his mono tubs. [Re: MOPE] 2
#14364207 - 04/27/11 10:46 PM (12 years, 8 months ago) |
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Solid.
-------------------- Treat my posts like the Bible. I am fallible, subject to human limitation, and usually open to interpretation.
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skiddy
RockStar


Registered: 03/25/11
Posts: 366
Last seen: 12 years, 6 months
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Re: How 13shrooms does his mono tubs. [Re: Luger0815] 2
#14364252 - 04/27/11 10:51 PM (12 years, 8 months ago) |
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Bookmarked very nice
-------------------- PESH : Pinning Transeski : colonizing Orrisa : colonizing Mex a : colonizing You're not a mycologist just because you grow mushrooms.
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Barakanaten
Ama-gi



Registered: 04/14/10
Posts: 6,163
Loc: PNW Mycosphere( Blessed C...
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Re: How 13shrooms does his mono tubs. [Re: Masticore] 2
#14364266 - 04/27/11 10:53 PM (12 years, 8 months ago) |
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--------------------
New to cultivation? Have a Question? Because AMU We Q&A Know AMU Genetic Library Easy Felt  AMU  Official PNW Mushroom Hunting Season 2011"...Though in all else he may be as Saintly as St. Francis, in the pursuit of these ends he is more Satanic than Satan. He will betray his nearest and dearest without the slightest twitch of flesh or spirit. He is amoral." -Definition of a mushroom hunter by ~Angelo Pellegrini
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schmoopy



Registered: 10/23/10
Posts: 462
Loc: PNW
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Re: How 13shrooms does his mono tubs. [Re: Masticore] 2
#14364348 - 04/27/11 11:08 PM (12 years, 8 months ago) |
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Very concise and helpful! 
-------------------- The observer is the observed. -J. Krishnamurti
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psyeye
looking looking looking



Registered: 04/12/11
Posts: 136
Loc: Sydney AU
Last seen: 8 years, 5 months
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Re: How 13shrooms does his mono tubs. [Re: Barakanaten] 2
#14364356 - 04/27/11 11:09 PM (12 years, 8 months ago) |
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gorgeous thread 13shrooms, thanks for the GLC workflow... it's next on my list for sure
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Re: How 13shrooms does his mono tubs. [Re: Javadog] 2
#14364426 - 04/27/11 11:22 PM (12 years, 8 months ago) |
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Great tek, goes perfect with D5050's coir and agar's GLC and it saves the waste from making a GLC.
How do you get enough liquid out for it to re colonize, let them sit tilted towards the noc hole and come back after it drains and extract more liquid from it?
Also in your first steps it doesn't go into detail about how much spawn is in your large filter patch spawn bag and how it is preparedDerr it's in the GTG link, thx 13. Sorry, is there a link in AMU's links to it I should have looked before I started babbling. You have enough links in this post, I imagine that it toook quite a while, THANKS!, I will check AMU's threads now.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
Edited by 3n1gm4 (04/27/11 11:30 PM)
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: How 13shrooms does his mono tubs. [Re: psyeye] 2
#14364433 - 04/27/11 11:23 PM (12 years, 8 months ago) |
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casual
Stranger(intraining)

Registered: 04/05/11
Posts: 128
Loc: Somewhere decent.
Last seen: 7 years, 9 months
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Re: How 13shrooms does his mono tubs. [Re: anonjon] 2
#14364529 - 04/27/11 11:41 PM (12 years, 8 months ago) |
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Awesome tek man very clean. Bookmarked for future reference
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chinamon
Stranger
Registered: 01/19/11
Posts: 66
Last seen: 12 years, 1 month
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Re: How 13shrooms does his mono tubs. [Re: anonjon] 2
#14364543 - 04/27/11 11:44 PM (12 years, 8 months ago) |
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where can i get the lids in the OP?
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
Loc: The Downside of Up
Last seen: 12 years, 7 months
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Re: How 13shrooms does his mono tubs. [Re: chinamon] 2
#14364615 - 04/28/11 12:00 AM (12 years, 8 months ago) |
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The AMU TEK link in 13's sig under todlow's name. Not exactly the same but pretty much. Those lids are on AMU's first page in 13shrooms journal I think, TLs are better IMO.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: How 13shrooms does his mono tubs. [Re: 3n1gm4] 2
#14364684 - 04/28/11 12:17 AM (12 years, 8 months ago) |
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Quote:
3n1gm4 said: The AMU TEK link in 13's sig under todlow's name. Not exactly the same but pretty much. Those lids are on AMU's first page in 13shrooms journal I think, TLs are better IMO.

noobieshroomie and TL both have great lid teks. 

--------------------
A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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UnnamedGrower
The AMUiest



Registered: 04/17/11
Posts: 7,146
Loc: I'll be where I'm at
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Re: How 13shrooms does his mono tubs. [Re: Barakanaten] 2
#14365349 - 04/28/11 06:18 AM (12 years, 8 months ago) |
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Quote:
Barakanaten said: Nice fruits!
Great job 

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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: How 13shrooms does his mono tubs. [Re: UnnamedGrower] 2
#14388314 - 05/02/11 12:23 PM (12 years, 8 months ago) |
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--------------------
A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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Barakanaten
Ama-gi



Registered: 04/14/10
Posts: 6,163
Loc: PNW Mycosphere( Blessed C...
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Re: How 13shrooms does his mono tubs. [Re: UnnamedGrower] 2
#14389283 - 05/02/11 03:30 PM (12 years, 8 months ago) |
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Quote:
UnnamedGrower said:
Quote:
Barakanaten said: Nice fruits!
Great job 


Man what a great tek, very informative, thanks for all the info 13, this is gold
--------------------
New to cultivation? Have a Question? Because AMU We Q&A Know AMU Genetic Library Easy Felt  AMU  Official PNW Mushroom Hunting Season 2011"...Though in all else he may be as Saintly as St. Francis, in the pursuit of these ends he is more Satanic than Satan. He will betray his nearest and dearest without the slightest twitch of flesh or spirit. He is amoral." -Definition of a mushroom hunter by ~Angelo Pellegrini
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