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OfflineItheus
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sterilize vs pasteurize moldy coffee
    #14363455 - 04/27/11 08:39 PM (12 years, 9 months ago)

So, once more I'm at an impasse; I've exhausted the search function here, only finding answers to different questions. I'm about to do a straight coffee ground (and a bit of verm to even things out, spawning from WBS) grow of P. ostreatus. For the past two weeks instead of trashing my coffee grounds (not flavoured or artificial-ized in any way) I've been putting them in a tabletop compost thing I have, which is just a  big ceramic jar with a filter to keep out bugs, but that's mostly irrelevant.

So I dumped all these in a spawn bag, and the bottom of this coffee collection, it was a bit white and moldy. Now I =was= gonna pasteurize it and spawn the WBS to it, and I really do prefer pasteurization to sterilization when I can do it, but that mold has me doubting my methods.

Should I pasteurize or sterilize this shiz? And why? is my real question. I've always sterilized grains and pasteurized casings, straw and manure, but never really known why. (Except that grains must obviously be sterilized, and pasteurizing bulk substrates allow beneficials to aid in preventing contamination of the substrate.) But what about BRF for example, or anything without a grainy core, why not just pasteurize? And how effective is pasteurization against a straight up moldy bulk substrate? Or should I just toss that coffee? Oh gosh, I just need to f***ing know so I can stop bugging people with such ridiculous questions.

I guess this question is aimed toward the experimental circumstances surrounding the use of an unknown/experimental bulk substrate, and whether to sterilize or pasteurize, and for what exact reason. Whaddya say?


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Re: sterilize vs pasteurize moldy coffee [Re: Itheus]
    #14363498 - 04/27/11 08:47 PM (12 years, 9 months ago)

I've read of PC'ing compost bags just like grain and using LC to knock it up like a spongie block or a large cake... Never tried it myself though


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Re: sterilize vs pasteurize moldy coffee [Re: Itheus]
    #14363509 - 04/27/11 08:49 PM (12 years, 9 months ago)

I would suggest visiting your local Starbucks, especially after the am rush they will give you loads of fresh, mold free grounds free of charge. I wouldn't personally inoculate anything I knew had mold in it I wouldn't even bother  pasteurization just toss it and get some fresh ones If possible. And as far as pasteurizing your bulk sub I recommend searching "bucket pasteurization" it's very simple and I have had great success with no contams so far.


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OfflineItheus
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Re: sterilize vs pasteurize moldy coffee [Re: Soulfull]
    #14363526 - 04/27/11 08:53 PM (12 years, 9 months ago)

Yeah pasteurization is easy-peasy-lemon-squeazy, and so is finding coffee grounds. And truth is, I'm not tossing it; if I can't pasteurize it, I'm gonna sterilize it. And my question is not restricted to the aforementioned circumstances, I'm asking a general question to which I could not find answers using the search function. So no more "here's how ya do it" answers, okay?


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Re: sterilize vs pasteurize moldy coffee [Re: Itheus]
    #14363564 - 04/27/11 09:01 PM (12 years, 9 months ago)

You pasteurize bulk substrates & additives of such due to keeping good microbes around that'll stave off infection from contams.

You sterilize to ensure your spores/LC/G2G will take & give the best chance for you mushies to colonize it.

ALWAYS PROPERLY pasteurize bulk substrates & additives for the aforementioned reasons, same as sterilizing.

As for your current situation, I'd ditch the moldy stuff & start anew w/ it. People are able to use compost, because it stays @ a temperature that will keep away some contams & is still pasteurized before use.

If absolutely HAVE to use that moldy coffee, which if it's white is cobweb mold due to lack of GE @ the bottom of your bag, make sure you pasteurize the coffee for 1 hour ATLEAST...I'd go 90 minutes just to be sure
:2cents:


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OfflineItheus
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Re: sterilize vs pasteurize moldy coffee [Re: TranscendingLife]
    #14363616 - 04/27/11 09:10 PM (12 years, 9 months ago)

Well I'm glad we generally agree, but my real question goes unanswered. I guess it's RR I want an answer from. (*hopeful*)

Really though, this is not a question I'm asking because I want "success." I will be successful if I can find answers and learn something; I'm really not worried about how this grow goes, I've got plenty of happy, healthy oysters growing at the moment, and I couldn't eat another. As with all valuable mycological efforts, science comes before produce.


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Re: sterilize vs pasteurize moldy coffee [Re: Itheus]
    #14363656 - 04/27/11 09:16 PM (12 years, 9 months ago)

Update:

In reading up the good literature on the subject (no answers to my exact question, of course) I found that the practice of fermenting the coffee grounds is common; does anyone know anything about that? I really have no idea what they mean by "ferment" but I'm somehow doubtful that they're referring to S. cerevisiae. Anyone got any input on that bit?


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Re: sterilize vs pasteurize moldy coffee [Re: Itheus]
    #14363701 - 04/27/11 09:25 PM (12 years, 9 months ago)

if your storing your coffee grounds for later use you have to dry them out otherwise they will grow mold.

Pasturising will kill of any mold spores and mold, but it wont get back what the mold has eaten.


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Re: sterilize vs pasteurize moldy coffee [Re: veda_sticks]
    #14363709 - 04/27/11 09:26 PM (12 years, 9 months ago)

Vraiment? Ouay.


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