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OfflinePulmonarius
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Pleurotus Pulmonarius Grow on mixed substrate
    #14351562 - 04/25/11 08:43 PM (12 years, 10 months ago)

Hi all!

Being on old member of this forum, I stopped mushroom growing for a while now (my last print was made in 04!), I just got some pulmonarius oysters spores a few weeks ago.. Directly multispored to WBS, then G2G to larger jars, I had 3 liters of WBS spawn to play with..

Not being able to easily source straw, I tried a mix of various substrates, namely: coco coir, newspaper, cardboard  (toilet rolls), hamster litter (looks like coniferous wood shavings), and coffee grounds.

These were mixed in uncalculated proportions, but majorly coco coir and newspaper/carboard, then pastereuzied in two ways: first, adding the correct amount of water to the mixed ingredients, placing them in 1L jars, that were then immersered in 650C water for 3h then let to cool to room temp, and second by placing the ingredients in a pillow case and submerging in 65°C water for 2H, then leaving to drip and cool for a few hours until room temp.

The first go seems to be getting colonizing very quick, because after inoculation with 20% spawn, in a bleach-cleaned plastic box and in a 1L jar,  it's already about half colonized after only 2 days! Very very agressive species..

The second go is cooling as I type, and will be inoculated with the same % of spawn in a large transparent bucket.

I will take pictures tomoroow morning.

Fruitng chamber is a mini-greenhouse indoors, with a ultrasonic humidifier set to work 15min per hour, with a air pump bubbling through a jar of water with a little H202 in it.

Ah, half the spawn jar were treated with H2O2 to see if it made a difference, as I was worried there was a slight bacterial contam, which apparently turned out just to be metabolites.. No contam at all in any of the jars, and no difference in growth.


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Pulmonarius Embolism

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Offlinedeadmandave
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14352715 - 04/25/11 11:28 PM (12 years, 10 months ago)

Looking forward to some pictures as well as progress :biggrin:

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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: deadmandave]
    #14357911 - 04/26/11 09:38 PM (12 years, 10 months ago)

Ok!

Well, the jar and first plastic box are already nearly 100% colonized! In 3 days! Pictures are uploading..

I also prepared the large PP bucket, but it seems pretty humid from my point of view.. We will see how the mycelium handles such an environment, hopefully the pasteurized substrate will resist contam the time the mycelium gets strong again..  Several holes were made in the lid, and the bucket placed in a garbage bag, at 21°C (no incubator unfortunaly).

I'm also adding a picture of a 3 weeks old sawdust/ woodchips/ coco coir/ coffe grounds bag that is nearly finished colonizing, and will be fruited soon. The woodchips are pretty long to get completly colonized compared to coco, and the bag was lacking air exchange until a few days ago.

I'm not sure was I will do with the colonized pasteuzied substrate jar fruiting-wise, maybe just remove the lid and allow the fruitbodies to form on the surface, but that seems to be a waste for oysters, might be better to remove the "cake" from the jar, and place in a clean bag with holes, no? What are your advices?

I still have another jar of spawn left, not sure was to do with it, I might try pasteurized cococoir/coffe grounds with some vermiculite to air it up a little. I won't be using the pillow bag idea again and it seems to leave to much water in the substrate..

PICS:

Spawn on WBS, from multispore inoculation


Pasteurized substrate


First box:



First Jar after 3 days:


Woodchips bag:


10L PP bucket after inoculation:



Greenhouse:


Enjoy!



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Pulmonarius Embolism

Edited by Pulmonarius (04/26/11 10:31 PM)

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OfflineNSF
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14359260 - 04/27/11 01:37 AM (12 years, 10 months ago)

Just three days?!?!

I've had only slightly more colonisation in a month!!!


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OfflineMycelio
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14359794 - 04/27/11 07:07 AM (12 years, 10 months ago)

Sounds good and looks fine, only two comments.
While fruiting oysters, an aquarium pump with an air stone will never be sufficient for FAE. You will have to find a much stronger solution or just leave the door unzipped.
When fruiting such a box, the first flush might work out OK, but then the substrate will contract and following flushes will appear in the gap between the box and the substrate. These gaps are also a paradise for pests and contams. You will have more fun, fruiting in bags with holes for the fruitbodies.

Carsten

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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Mycelio]
    #14360729 - 04/27/11 11:47 AM (12 years, 10 months ago)

Ok, thanks for the advices. I will have to leave the door partially unzipped then, and keep the ultrasonic on a bit more often...

Unfortuanly, the bags are pretty expensive from the only local source I found, but i do have some buckets, aren't these often used for oysters?

Regarding the jar, should it be best to leave it as is with the lid of, or transfer it to a pasteurized bag?


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Pulmonarius Embolism

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Invisiblejwand6
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14361028 - 04/27/11 01:12 PM (12 years, 10 months ago)

I used the aquarium air pump/bubblestone setup for cubies, but it is not sufficient for oysters, huh? Good to know. 
Good luck pulmonarius


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Imagine, or just pretend to imagine.

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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Mycelio]
    #14361124 - 04/27/11 01:34 PM (12 years, 10 months ago)

Concerning the colonization I too am surprised! I have grown pink oysters in the past and it was much longer!! I did force a bit on the % of mycelium though, I usually turn around 5% and there it was closer to 15%...

I prepared another bucket with the last spawn jar last night !

How well do oysters perform by fruiting as a cake directly? I guess there a reason  they are always left in a bag or bucket... Constant and high humidity I suppose?

BTW, I have this small fan I use to cool the computer, about 20cm diameter, I could use this with some kind of filtration for FAE, no? Or would it be too much, even on a timer? hanks for your input.


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Pulmonarius Embolism

Edited by Pulmonarius (04/27/11 01:37 PM)

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OfflineMycelio
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14361140 - 04/27/11 01:36 PM (12 years, 10 months ago)

Fruiting them in cheap freezer bags works much better for me. Look for posts by punkin to see how he uses buckets.

@jwand6
Correct, in terms of fresh air, these species are kind of the opposite.

Carsten

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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Mycelio]
    #14361163 - 04/27/11 01:39 PM (12 years, 10 months ago)

OK, thanks. So I will put the colonized jar in a freezer bag. Should I crumble it and then leave it to resolidify itself, or just place it as is, and cut the bag accordingly?


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Pulmonarius Embolism

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OfflineMycelio
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14361255 - 04/27/11 01:55 PM (12 years, 10 months ago)

Both is possible. I usually crumble it up and mix in the same amount of pasteurized substrate repeatedly, but colonization in the bag requires some kind of air exchange.

Carsten

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OfflineNSF
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14362196 - 04/27/11 04:44 PM (12 years, 10 months ago)

I believe computer fans are good for fae, especially if you can attach it to some pipe with flashing.  You do want to humidify air you blow in though, dry air = dry substrate = light or few shrooms.  The water in the block is what provides the weight and juicyness in our end product.  The less water you sap from a block the better.

If you are mixing grain into pasteurised substrate still try and be as clean as possible.  It's not essential but it helps. People like to up their spawn ratio to get faster colonisation, kind of racing the contams.


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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14363124 - 04/27/11 07:30 PM (12 years, 10 months ago)

Hum, ok, so that would mean I should place the UltraSonic before the fan with some tubing.. That is going to take some engineerning as it's a floatable device... I guess I will try leaving the door unzipped and manually venting, and adding a second aqurium pump. If only my finaces could able me to get a CO2 measurement device..
I will have to get some perlite to add to the bottom of the greenhouse too..

The large PP bucket is showing signs of growth, I should ahve layered the mycelium rather than entirely mix the two though, might have save dsome time for the mycelium to get back at it..


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Pulmonarius Embolism

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OfflineNSF
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14363202 - 04/27/11 07:50 PM (12 years, 10 months ago)

Have a search around for fog bucket set ups, either ozzy or evil mushroom has a link in their sig to their setup.  One uses a computer fan to blow air into a sealed bucket where a fogger is working, this air and fog is then 'vented' into the grow space.

You could just make sure your air enters your grow space through the cool mists mist, blowing that around.

Also, you can mix through rather than layer, that makes for fast colonisation...i don't understand why you'd want to slow that down.


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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: NSF]
    #14381350 - 05/01/11 05:20 AM (12 years, 9 months ago)

Actaually, I find layering quickier than mixing, I mixed in the large PP bucket, but the mycelium took a couple of days to recover, while when layering, it recovers in matter of hours.. Of course I did small layers to compensate the slower colonization.

The 5L bucket is already 95% colonized, in a week! I also did a coco coir/cofee bucket, layered, which is nearly done in 5 days.. The box, bag and jar (placed in a freezer baggie with holes in it) seems to be slow at fruiting. I guess still not enough air exchange, even though the door is half unzipped.. Problem is the greenhouse is in my bedroom, and I get a runny nose from the humidity in the room..

I'm thinking of placing it outside, but I am scared of flies and other bugs will invade the greenhouse, especially with the dorr unzipped.. Anyone got experience with that?


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Pulmonarius Embolism

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OfflineMycelio
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14381484 - 05/01/11 06:43 AM (12 years, 9 months ago)

Don't fruit them in your bedroom! That would be the most successful way to get allergic to their spores for the rest of your life.

You better look for a way to do colonization inside and fruiting outside. You should be able to get three flushes, before the gnats multiply too much. Outdoor fruitings are usually much better, while the pests do less damage.

Carsten

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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Mycelio]
    #14387727 - 05/02/11 10:16 AM (12 years, 9 months ago)

Yes, I've just realized that.. SO I placed most of the blocks outside, in a shady place that gets direct sunlight in the morning for a couple of hours.. I'm not sure how to keep the humidity up , I placed the blocks on a layer of vermiculite with a plastic bag covered, leaving enough space to breath, and moisterizing them two times a day..

I'm thinking of moving the greenhouse outside, but not in the garage as there are tons of flies in there in the summer..

Does king oysters and pink oysters pose the same allergy problem? I read that they sporulate much less..


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Pulmonarius Embolism

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OfflineMycelio
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14387876 - 05/02/11 10:42 AM (12 years, 9 months ago)

My pinks are among the top spore producers, while the kings drop less, but that varies from strain to strain.

As I don't have a garden, I fruit my blocks in shaded greenhouses on the balcony. You can also dig holes, put the blocks in and cover them with soil. A cellar room should also be fine, though you may have to install artificial light.

Carsten

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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Mycelio]
    #14400659 - 05/04/11 03:47 PM (12 years, 9 months ago)

Thanks for the advice. I've placed the greenhouse outside, and removed the improvized sealing I made with bin bags to let fresh air come in, as it's pretty windy. The ultrasonic si turning 24/24H.

The bag was placed in a shaded garden spot, hopefully it will fruit before the gnats bite in, it has alreday formed scleatorias, I'm hoping this will not cause rotting. I placed a clear plastic bag over it to keep things humid.

Pictures to come.


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Pulmonarius Embolism

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OfflinePulmonarius
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Re: Pleurotus Pulmonarius Grow on mixed substrate [Re: Pulmonarius]
    #14425261 - 05/09/11 03:42 PM (12 years, 9 months ago)

I don't get it. None of my pulmonarius cultures are pinning. There nearly all formed scleratoria, put no pins. They are outside, there is a lot of wind to give plenty of fresh air, humidity is a bit low 70-80%, and temp are between 15°C at night and 25 max during daytime.. Is the humidity the probleme? I remember fruiting them at 75% in th epast.. I'm thinking of chucking everything in the garden, under earth, with some newspaper etc and covering with pastic bag and watering everyday and hoping for a crop before summer really kicks in..

What could be the problem?


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Pulmonarius Embolism

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