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Offlineoysterlover
Stranger

Registered: 04/19/11
Posts: 166
Last seen: 5 years, 5 months
LC contam
    #14360206 - 04/27/11 09:29 AM (12 years, 9 months ago)

So I made an LC....which I have never been successful with before.  Ended up with 2 jars, and shot one with some really old myc i had stored in distilled water w/ h2o2 in the fridge for over a year...the other with some old spore solution.

Fast forward a couple weeks, and the spore jar shows no growth, while the myc jar shows BEAUTIFUL white fluff.  I extract a little with a syringe and shoot to agar....and get a nice fast growinig green jar.

Now should I assume that the LC is actually an LC of trich, or should I try again and assume that my transfer was to blame?  Is there a contam that would grow in an LC and look EXACTLY like good myc?  I'll try to get pics if anyone thinks there's a chance it's still good....otherwise I'm flushing it.


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Contaminate is a verb.  Contaminant is a noun.
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Invisiblewow
Lurking master
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Registered: 07/27/06
Posts: 1,260
Loc: Cloud 9
Re: LC contam [Re: oysterlover]
    #14360314 - 04/27/11 09:55 AM (12 years, 9 months ago)

if you have other source for culture might as well flush it..  or try a test jar..


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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: LC contam [Re: oysterlover]
    #14360322 - 04/27/11 09:56 AM (12 years, 9 months ago)

Is the distilled water/H2O2 the LC you're referring to?  If so, the H2O2 could be the reason you can't tell the difference between trichoderma and mycelium.  LC fungi puffballs can be hard to differentiate without visible spores.  If there's H2O2 in the mix, it can be bleaching the green spores.


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Offlineoysterlover
Stranger

Registered: 04/19/11
Posts: 166
Last seen: 5 years, 5 months
Re: LC contam [Re: Sillicybin]
    #14360816 - 04/27/11 12:13 PM (12 years, 9 months ago)

No, the H2O2 was how I stored the myc....I transferred it to the LC container to revive.  I don't have any left....I was really hoping it would take.

I guess the real test would be to try it out on maybe 5 agar plates and see what I get.  If they all go green I'll toss em.


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Contaminate is a verb.  Contaminant is a noun.
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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: LC contam [Re: oysterlover]
    #14360829 - 04/27/11 12:18 PM (12 years, 9 months ago)

That's definitely not a bad way to go if saving the culture is worth it to ya.  Good luck!


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Offlineoysterlover
Stranger

Registered: 04/19/11
Posts: 166
Last seen: 5 years, 5 months
Re: LC contam [Re: Sillicybin]
    #14361487 - 04/27/11 02:41 PM (12 years, 9 months ago)

Looks like I'll be cooking up some agar tonight!


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Contaminate is a verb.  Contaminant is a noun.
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