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Vibration
Student


Registered: 08/23/10
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Last seen: 7 years, 4 months
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My 5L pressure cooker and my failure with WBS > success with WBS
#14359610 - 04/27/11 05:19 AM (12 years, 10 months ago) |
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My last pressure cooker stopped working and I quickly had to buy some random pc for low price from an auction locally, because I didn't have enough resources to buy a descent one and more importanly - I couldn't wait.
So I got this. All I know about it: It's 5L and there is "Domotti" written on the box it came with.
I'm writing this, because I can't figure out what went wrong with my last 12 jars of wbs when I inoculated them. Only one of them got tiny bit of growth (which I noticed after 18 days from inoculation).
Few months ago I made a successful grow using the same method with wbs jars (it was my first time) and now when I did the same thing I basically failed with all the jars.
Two things which was different now: 1) I got different pressure cooker 2) When I prepared wbs, I soaked it for 48 hours instead of 12 hours
When I opened the jars today, I took a little sniff and there was clearly a sour smell coming out of them. It wasn't too intense though. Could it be bacteria?
When I sterilized the jars, I cooked 60-65 minutes each one. One thing that really sucks with the new pressure cooker is the fact that I literally had to pc the jars one by one, because I can only fit one quart sized jar at once in the cooker.. and now I will have to sterilize another 14 jars again. <--- Because of that I'm really hoping to succeed this time, cos otherwise I will waste lots of time.
My first jar is already cooking. I increased the cooking time to 75 minutes.
Do you have anything to say to me which could improve my situation in any way? I'm feeling quite insecure.
-------------------- The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.
Edited by Vibration (05/13/11 06:45 AM)
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Mycowlogist
Freethinker



Registered: 06/29/08
Posts: 534
Loc: in a galaxy far far away....
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Re: My 5L pressure cooker and my failure of WBS [Re: Vibration]
#14359621 - 04/27/11 05:29 AM (12 years, 10 months ago) |
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Most people PC their grain for 90 Minutes. In my opinion, I wouldn't continue until you get a better PC. It's too small to work with, you are wasting a lot of electricity ($), does it reach 15 PSI? (don't see pressure gauge).
Doesn't matter if you do everything else right. If you don't properly PC your grain, you will have a have high contamination ratio.
Check out Goodwill stores, Yard Sales, Craig list, Nana's kitchen etc.
-------------------- "And in the end, it's not the years in your life that count. It's the life in your years." Honest Abe
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Slayer1024
Stranger Shroomer



Registered: 03/08/11
Posts: 397
Loc: At his place ^^
Last seen: 9 years, 7 months
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Re: My 5L pressure cooker and my failure of WBS [Re: Vibration]
#14359654 - 04/27/11 05:51 AM (12 years, 10 months ago) |
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I only have a 6L pressure cooker myself, and I can get 4 pint jars in mine its not ideal but its better than 1 jar at a time and I always PC for 90 mins because mine only hits 11.5 PSI. I only soak for 12 hours and toss the WBS in the PC. Although I'm pretty new to this I have only had 1 jar get contams, using this method
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: My 5L pressure cooker and my failure of WBS [Re: Mycowlogist]
#14359664 - 04/27/11 05:56 AM (12 years, 10 months ago) |
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Quote:
Mycowlogist said: Doesn't matter if you do everything else right. If you don't properly PC your grain, you will have a have high contamination ratio.
Completely true. OTOH, if your syringe is bad, doesn't matter how sterile the grain is.
-------------------- You make it all possible. Doesn't it feel good?
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Vibration
Student


Registered: 08/23/10
Posts: 1,053
Last seen: 7 years, 4 months
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Re: My 5L pressure cooker and my failure of WBS [Re: Doc_T]
#14359707 - 04/27/11 06:24 AM (12 years, 10 months ago) |
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Ok, I believe mine doesn't reach 15psi either. I will have to raise the cooking time to 90 minutes then.
And now I'm really paranoid about my LC. To be honest, I thought when LC gets contaminated by bacteria, then water gets cloudy inside and mycelium doesn't grow, but mine grew really fast and water was super clear.
(early days)

And I see people always saying: You have to test your LC on BFR, because some "things" doesn't show off before inoculating.
For some reason I've always thought these things are mold, but not bacteria. And when testing LC, how can I be sure if it's bad or good? If mycelium starts growing, it's good. If mold starts growing, it's bad, but what if there isn't going to be any signs of any growth?
Which way does it work exactly, can anyone explain Please?
-------------------- The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: My 5L pressure cooker and my failure of WBS [Re: Vibration]
#14359713 - 04/27/11 06:26 AM (12 years, 10 months ago) |
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Little PCs work great, BTW. But follow the instructions and give the full time.
Quote:
And when testing LC, how can I be sure if it's bad or good?
Really, you can't. Biggest reason I stopped using the stuff for most applications. You can sometimes tell that it's bad- but there's no way to prove it's not bad.
-------------------- You make it all possible. Doesn't it feel good?
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Vibration
Student


Registered: 08/23/10
Posts: 1,053
Last seen: 7 years, 4 months
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Re: My 5L pressure cooker and my failure of WBS [Re: Doc_T]
#14359721 - 04/27/11 06:32 AM (12 years, 10 months ago) |
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So does it mean there is a reasonable possibility of my liquid culture being contaminated by bacterial form, and this could be the whole reason why my 12 jars of wbs have failed?
If I got that right, then thanks for the lesson.
-------------------- The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: My 5L pressure cooker and my failure of WBS [Re: Vibration]
#14359724 - 04/27/11 06:37 AM (12 years, 10 months ago) |
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LC sucks. (Edit- but the one in the pic looks ok, shoot a test jar. )
Buy a pack of agar for $1.69, do the job right the first time.
-------------------- You make it all possible. Doesn't it feel good?
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Vibration
Student


Registered: 08/23/10
Posts: 1,053
Last seen: 7 years, 4 months
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Re: My 5L pressure cooker and my failure of WBS [Re: Doc_T]
#14359803 - 04/27/11 07:09 AM (12 years, 10 months ago) |
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I think I have few last things to ask on this subject.
At the moment I'm in a process of sterilizing wbs jars, and by tomorrow afternoon this process will be finished 
Okay, but I have some options to choose between and I'm quite schizophrenic decision maker.
Yesterday I inoculated my test jar:

Can I place my sterilized wbs jars in the closet and wait for the first signs of growth in my lc jar, or is it a bad idea to let the wbs jars sit like that.
If that's a bad idea, should I make Grain To Grain Transfer using this jar?

Keep in mind that it has been sitting like that for 3 months in my closet in room temperatures (not a problem?).
What would you do in my situation?
-------------------- The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
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Re: My 5L pressure cooker and my failure of WBS [Re: Doc_T]
#14359925 - 04/27/11 08:02 AM (12 years, 10 months ago) |
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Quote:
Doc_T said: Little PCs work great, BTW. But follow the instructions and give the full time.
Quote:
And when testing LC, how can I be sure if it's bad or good?
Really, you can't. Biggest reason I stopped using the stuff for most applications. You can sometimes tell that it's bad- but there's no way to prove it's not bad.
If you make the LC from a visibly clean 100% colonized grain jar GLC then aren't you sure that the LC is also good as long as you followed sterile procedures? If the GLC is clean it couldn't contam the LC unless the contam got in through the filter or the noc process, right?
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: My 5L pressure cooker and my failure of WBS [Re: 3n1gm4]
#14359963 - 04/27/11 08:14 AM (12 years, 10 months ago) |
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LC is not Grain LC. Also, until you've taken it from syringe all the way to fruiting, you really can't say it's good.
-------------------- You make it all possible. Doesn't it feel good?
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
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Re: My 5L pressure cooker and my failure of WBS [Re: Doc_T]
#14360005 - 04/27/11 08:27 AM (12 years, 10 months ago) |
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I was speaking of shooting a few cc of good GLC into a LC jar and letting it grow from the GLC.
-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: My 5L pressure cooker and my failure of WBS [Re: 3n1gm4]
#14360034 - 04/27/11 08:36 AM (12 years, 10 months ago) |
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What about it? That doesn't prove the LC is clean. You could drop in an agar wedge. It still does not prove the LC is clean.
-------------------- You make it all possible. Doesn't it feel good?
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Mycowlogist
Freethinker



Registered: 06/29/08
Posts: 534
Loc: in a galaxy far far away....
Last seen: 10 years, 1 month
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Re: My 5L pressure cooker and my failure of WBS [Re: 3n1gm4]
#14360263 - 04/27/11 09:43 AM (12 years, 10 months ago) |
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Quote:
3n1gm4 said: I was speaking of shooting a few cc of good GLC into a LC jar and letting it grow from the GLC.
In theory that would be a clean LC.
-------------------- "And in the end, it's not the years in your life that count. It's the life in your years." Honest Abe
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: My 5L pressure cooker and my failure of WBS [Re: Mycowlogist]
#14360273 - 04/27/11 09:45 AM (12 years, 10 months ago) |
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No, in theory and in actual fact, that would be an untested LC.
-------------------- You make it all possible. Doesn't it feel good?
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Vibration
Student


Registered: 08/23/10
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Re: My 5L pressure cooker and my failure of WBS [Re: Doc_T]
#14360342 - 04/27/11 10:01 AM (12 years, 10 months ago) |
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None of the options I've mentioned in my last post are acceptable? I'm fucked.
-------------------- The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: My 5L pressure cooker and my failure of WBS [Re: Vibration]
#14360486 - 04/27/11 10:39 AM (12 years, 10 months ago) |
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What was the question?
-------------------- You make it all possible. Doesn't it feel good?
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3n1gm4
3N!9M4T!C



Registered: 01/13/11
Posts: 2,727
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Re: My 5L pressure cooker and my failure of WBS [Re: Doc_T]
#14361014 - 04/27/11 01:09 PM (12 years, 10 months ago) |
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-------------------- http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!
   
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Vibration
Student


Registered: 08/23/10
Posts: 1,053
Last seen: 7 years, 4 months
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Re: My 5L pressure cooker and my failure of WBS [Re: 3n1gm4]
#14365531 - 04/28/11 07:26 AM (12 years, 10 months ago) |
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Thank you very much, enigma. Look, this is my 3 months old wild bird seed jar. Does it seem healthy? There are some weird spots, but it seems like a thick mycelium to me though.
I think I will need to tranfer some grain from that jar to another jar in a glovebox and with a spoon.. cos that particular jar is too full and mycelium is too thick in my opinion. Do you agree?
I will start with the process right away when I get 1 reply regarding this matter 





-------------------- The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: My 5L pressure cooker and my failure of WBS [Re: Vibration]
#14365536 - 04/28/11 07:27 AM (12 years, 10 months ago) |
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Looks suspicious. Maybe ok, but looks a bit bacterial to me.
-------------------- You make it all possible. Doesn't it feel good?
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