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OfflineMushaboom
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Malt Extract Agar LC
    #14356886 - 04/26/11 06:22 PM (12 years, 10 months ago)

This evening after work I will be attempting to make a LC using Malt Extract Agar. It says to mix 25g of MEA with 500ml of water and sterilize for 35min. at 15 PSI. I need to figure out how much MEA to use in order to have a not so viscous LC to use. Maybe 7.5g of MEA with 500ml of water? I am doing this because I have had poor results using any other method of creating an LC. I'm really good at everything else but when it comes to LC's I'm just not so good at it for some reason. Anyone have any suggestions?


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"What you think, you become."

Edited by Mushaboom (04/26/11 08:30 PM)

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Re: Male Extract Agar LC [Re: Mushaboom]
    #14356902 - 04/26/11 06:24 PM (12 years, 10 months ago)

Male Extract? gunna fap in it?


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Offlinegofudgeyourself
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Re: Male Extract Agar LC [Re: argg]
    #14356993 - 04/26/11 06:42 PM (12 years, 10 months ago)

argg your avatar almost made me shit myself lol.


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Re: Male Extract Agar LC [Re: gofudgeyourself]
    #14357008 - 04/26/11 06:46 PM (12 years, 10 months ago)

to the OP, LME looks cloudy at first and after PCing is a nice gold color with 0 sediment (i had sediment the first time, washed out jars and tried again, 0 sediment). it turns clear as the myc colonizes, best LC ever.

the recipe converts to 2 cups water + 1/2 tsp LME for those without proper measuring tools.

pro tip : use pint jars with chunks of glass in the bottom to cut up the myc. only fill the jars 1/4 or less with LC. make 2 ports on the top, cover 1 with micropore tape and seal the other one with red RVT automotive silicone. when PCing, cover with foil like normal jars and PC for a half hour.

good luck.


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"believe it if you need it, or leave it if you dare."

"turn all the hate in the world, into a mocking bird. make it fly away" :peace:

Edited by gofudgeyourself (04/26/11 06:52 PM)

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OfflineMushaboom
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Re: Male Extract Agar LC [Re: argg]
    #14357059 - 04/26/11 07:02 PM (12 years, 10 months ago)



Here is the MEA with some fresh fudge easter eggs my sister brought home for me. She works as a pastry chef so I get all kinds of treats when she gets home from work.


Here is the top of the self made lids I made for myself. I used tyvek for the filter, do you think these will work okay for my LC? I usually use these for my Rye Jars.


Could I use a quart jar like this with the measurements you have provided me with 2 cups and 1/2 teaspoon MEA?


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"What you think, you become."

Edited by Mushaboom (04/26/11 07:13 PM)

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OfflineMushaboom
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Re: Male Extract Agar LC [Re: argg]
    #14357505 - 04/26/11 08:30 PM (12 years, 10 months ago)

Quote:

argg said:
Male Extract? gunna fap in it?



haha thanks for pointing that out


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"What you think, you become."

Edited by Mushaboom (04/26/11 08:30 PM)

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Re: Male Extract Agar LC [Re: Mushaboom]
    #14357557 - 04/26/11 08:38 PM (12 years, 10 months ago)

I have an LME LC going and I hated it at first... lots of sediment in the bottom and it was really dark.  I just looked at it today and there's no sediment, the amber color is much lighter, and there's a huge puffy cloud in the middle.  Now I love it.

I used Doc_T (the LC hater)'s LC tek in his journal.


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Re: Male Extract Agar LC [Re: Trippy_Smurf]
    #14357818 - 04/26/11 09:23 PM (12 years, 10 months ago)

okay I've got it in a quart jar in the PC now.


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Offlinegofudgeyourself
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Re: Male Extract Agar LC [Re: Mushaboom]
    #14358565 - 04/26/11 10:57 PM (12 years, 10 months ago)

Keep in mind that you want to avoid any liquid from touching your filter...your lids look great, should work, let us know how it turns out. :smile:


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"believe it if you need it, or leave it if you dare."

"turn all the hate in the world, into a mocking bird. make it fly away" :peace:

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Re: Male Extract Agar LC [Re: Trippy_Smurf]
    #14359678 - 04/27/11 06:06 AM (12 years, 10 months ago)

Quote:

Trippy_Smurf said:
I used Doc_T (the LC hater)'s LC tek in his journal.




Even better if you make LC Lids: http://www.shroomery.org/forums/showflat.php/Number/11491601#11491601
(I don't do the tyvek any more, just polyfil. Shake carefully.)


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Re: Male Extract Agar LC [Re: Doc_T]
    #14359826 - 04/27/11 07:20 AM (12 years, 10 months ago)

Doc, how long should it take to see something in your LC when started from MS?  My agar->LC blew up fast, but my 4 from MS (different MSs) haven't done anything in a few weeks.


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Re: Male Extract Agar LC [Re: Trippy_Smurf]
    #14359968 - 04/27/11 08:17 AM (12 years, 10 months ago)

Are you asking how long to see growth from spores? 7-14 days.
I usually give LC jars about as long as any other jar, 3-4 weeks.
But with the Thick 'n' Chunky LC, you'll be able to tell when it's done, it'll eat all the stuff and the myc will settle.


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Re: Male Extract Agar LC [Re: Doc_T]
    #14360906 - 04/27/11 12:40 PM (12 years, 10 months ago)

Well it's done and inoculated. I can see the spores it's a medium light amber color. But I think it's going to work this time. I have 40 PF cakes no contamination and 12 Rye grain jars no contamination. So I believe this LC should work pretty well. I have 3 sleeves of agar dishes so I will be isolating again. I'm just now getting back into it. I used to grow about 3-4 lbs. a month with monotubs and cakes. I'm hoping to get back to that level because I lost all of my slants/isolates. Sucks but I'll get there eventually.


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Re: Male Extract Agar LC [Re: Mushaboom]
    #14373854 - 04/29/11 05:34 PM (12 years, 10 months ago)


12 qt jars of grain (not all shown in picture), 40 pf cakes (clear colonization started at 2 days :smile: I've had the spores for a while and the generation is a very old B+ strain grown over and over and over on pf cakes and grain, no contams appearing yet. Looks good so far.


Here is the LC in the light.


Here is the LC on the table, it's getting lighter than when I first made it. It was a dark amber color at first. Let me know what you guys think. Thanks, constructive criticism is encouraged heh.


--------------------


"What you think, you become."

Edited by Mushaboom (04/29/11 05:38 PM)

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Re: Male Extract Agar LC [Re: Mushaboom]
    #14375403 - 04/29/11 11:30 PM (12 years, 10 months ago)

BUMP: Should I try to stir up the LC from time to time? Or should I just let it sit?


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Re: Male Extract Agar LC [Re: Mushaboom]
    #14375426 - 04/29/11 11:37 PM (12 years, 10 months ago)

I'm not sure.
You want to shake or stir before you withdraw the fluid, but I'm not sure it's useful during the LC growth period.


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Re: Male Extract Agar LC [Re: Doc_T]
    #14375439 - 04/29/11 11:41 PM (12 years, 10 months ago)

I'll just leave it alone for now, stir whenever I take a look at growth which isn't too often. I don't like to mess with things too much, just keep it simple.


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Re: Male Extract Agar LC [Re: Mushaboom]
    #14375459 - 04/29/11 11:46 PM (12 years, 10 months ago)

Sounds good.Break it up well before you try to put it in syringes.


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Re: Male Extract Agar LC [Re: Doc_T]
    #14435297 - 05/11/11 02:56 PM (12 years, 9 months ago)

The LC was a complete Success. I inoculated 6 rye jars about 5cc's per jar. It's day 2 and and there has already been some growth. My LC is pretty viscous so I had to inject some warm water before drawing it up into the syringe. Very easy to do, considering all of my LC's using other ingredients were a complete failure. Next time I'm probably going to use a little bit more water to heighten the viscosity.


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