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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Is this normal for LC?
#14354560 - 04/26/11 11:06 AM (12 years, 9 months ago) |
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I used my first LC jar last Thursday to inoculate 16 BRF jars. I looked at them for the first time yesterday and they all have the shape of mycelium growth but it is incredibly light. Will this thicken up as time goes on or is this a contaminate. It just strikes me as odd that every jar would have this odd look if it was a contaminate, but I could be wrong. So this is a total of 5 days after inoculation. I used about 1cc-1.5cc's per jar.
  
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Is this normal for LC? [Re: Sarfix]
#14354582 - 04/26/11 11:12 AM (12 years, 9 months ago) |
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There's something growing in your LC.
-------------------- You make it all possible. Doesn't it feel good?
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Re: Is this normal for LC? [Re: Doc_T]
#14354593 - 04/26/11 11:14 AM (12 years, 9 months ago) |
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so these are all garbage yeah?
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Is this normal for LC? [Re: Sarfix]
#14354607 - 04/26/11 11:17 AM (12 years, 9 months ago) |
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Looks that way. 
Now you know why people say LC sucks. It's because LC sucks.
-------------------- You make it all possible. Doesn't it feel good?
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Maverick
Lover of Earwigs!




Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette
Last seen: 13 hours, 1 minute
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Re: Is this normal for LC? [Re: Doc_T]
#14354618 - 04/26/11 11:20 AM (12 years, 9 months ago) |
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Quote:
Doc_T said: Looks that way. 
Now you know why people say LC sucks. It's because LC sucks.
LC doesn't suck. People's technique's are what suck. Just because RR preaches that LC isn't good please don't jump on the bandwagon.
When doing an LC you should germinate the spores on agar first, to make sure you don't have contaminants from the beginning.
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Re: Is this normal for LC? [Re: Doc_T]
#14354624 - 04/26/11 11:21 AM (12 years, 9 months ago) |
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Lol for sure. I think I'm gonna give agar a shot today. Well one isn't truly agar.
I'm going to either use Agar Substitute or Grocery Store Agar Tek. Would you recommend either of those?
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Is this normal for LC? [Re: Maverick]
#14354634 - 04/26/11 11:23 AM (12 years, 9 months ago) |
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Quote:
Maverick said: When doing an LC you should germinate the spores on agar first, to make sure you don't have contaminants from the beginning.
It's not about RR.
You should germinate spores on agar, then transfer to grain, then make grain LC if you want liquid inoculant. That's the best practice by modern standards of home mycology.
-------------------- You make it all possible. Doesn't it feel good?
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Maverick
Lover of Earwigs!




Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette
Last seen: 13 hours, 1 minute
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Re: Is this normal for LC? [Re: Sarfix]
#14354640 - 04/26/11 11:24 AM (12 years, 9 months ago) |
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I'd suggest just ordering some MAE and good set of petris so that way you know what you're using's gonna work, however if you do the storebought way, just make sure to do proper ratios for agarpowder to malt. It's not super hard to do, you just have to find the right agar (a lot of asian places carry agar that's premixed with sugar, and that's not what you want).
Remember, a decent glovebox is a REQUIREMENT for agar work. You can't do it in any sort of ghetto tek, it's agar is just too much of a nutrient rich medium.
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Maverick
Lover of Earwigs!




Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette
Last seen: 13 hours, 1 minute
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Re: Is this normal for LC? [Re: Doc_T]
#14354647 - 04/26/11 11:25 AM (12 years, 9 months ago) |
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Quote:
Doc_T said:
Quote:
Maverick said: When doing an LC you should germinate the spores on agar first, to make sure you don't have contaminants from the beginning.
It's not about RR.
You should germinate spores on agar, then transfer to grain, then make grain LC if you want liquid inoculant. That's the best practice by modern standards of home mycology.
Who's best practices? I hate grain LCs, besides they don't expand. There's no problem going from agar->LC, you shouldn't get contams, your contam ratio should be identical to that of an agar->agar transfer. If it isn't, you're doing it wrong.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Is this normal for LC? [Re: Maverick]
#14354681 - 04/26/11 11:30 AM (12 years, 9 months ago) |
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Best practice by community standards, run a poll if you doubt it. I agree agar->lc is not inherently riskier than agar->agar. It's just harder to judge the results.
I expand my (glc or lc) by doing g2g. The syringe I use to start grain masters, then if they colonize I use them to make grain spawn. So a 20mL syringe of glc ends up being dozens of tubs of mushrooms.
-------------------- You make it all possible. Doesn't it feel good?
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Re: Is this normal for LC? [Re: Maverick]
#14354687 - 04/26/11 11:31 AM (12 years, 9 months ago) |
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It sure would be nice if those items were available to purchase in a store. I do have a decent glovebox so that shouldn't be a problem, I'm just sad these jars are all wasted oh well...I think I might go thrift store PC hunting today if I have to wait on the mail to bring me decent agar
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Is this normal for LC? [Re: Sarfix]
#14354693 - 04/26/11 11:32 AM (12 years, 9 months ago) |
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Find agar flakes at asian markets or local non chain health stores.
-------------------- You make it all possible. Doesn't it feel good?
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Re: Is this normal for LC? [Re: Doc_T]
#14354737 - 04/26/11 11:40 AM (12 years, 9 months ago) |
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Alright I have about $150 to spend on supplies today. So heres a few things I think I want to pick up. PC, Coffee Grinder, RTV silicone, Some more quart jars, Rye grain, a bag of organic brown rice, gypsum (if I can find it), and agar if I can find that as well.
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Re: Is this normal for LC? [Re: Sarfix]
#14354758 - 04/26/11 11:44 AM (12 years, 9 months ago) |
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Oh also, if I'm looking for a PC at a thrift store, should I be looking at places like goodwill and similar? or are they just clothes?
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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Maverick
Lover of Earwigs!




Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette
Last seen: 13 hours, 1 minute
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Re: Is this normal for LC? [Re: Doc_T]
#14354867 - 04/26/11 12:03 PM (12 years, 9 months ago) |
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Quote:
Doc_T said: Best practice by community standards, run a poll if you doubt it. I agree agar->lc is not inherently riskier than agar->agar. It's just harder to judge the results.
I expand my (glc or lc) by doing g2g. The syringe I use to start grain masters, then if they colonize I use them to make grain spawn. So a 20mL syringe of glc ends up being dozens of tubs of mushrooms.
Yes you're definitely right about judging results. It's almost impossible to knwo waht your isolate will do if it's directly from agar. A clone to agar then to LC seems more logical 
Also, I'd suggest buying yourself a Presto 17qt for around 30-50$ depending on where youf ind it on ebay. Thrift store PCs can be damaged (including microstructure damage, stress fissures in metal from years of use you can't see), they could be missing parts, or might not have a safety blowout pressure system.
If you do go for a thrift store one, make sure it's got a pressure gauge on the top and blowout seals. Don't get one of those 1970's pressurecookers.
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Re: Is this normal for LC? [Re: Maverick]
#14354919 - 04/26/11 12:11 PM (12 years, 9 months ago) |
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I own a 21.5quart All American Pressure cooker that I bought a few years ago for another purpose, but was never used. I never thought I would really have a use for it so when I moved out of my parents house I left it in their garage. Unfortunately we had kind of a falling out and I haven't talked to them in about 9 months. I spent a pretty penny on it though. My Dad is home right now due to unemployment so I could go over there. I'm trying to muster up some courage to just call and pick up some things. Ugh... it's a difficult situation worthy of forgetting about the couple hundred I spent on it, and buying a new one.
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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Maverick
Lover of Earwigs!




Registered: 12/18/05
Posts: 13,437
Loc: Valleys of Willamette
Last seen: 13 hours, 1 minute
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Re: Is this normal for LC? [Re: Sarfix]
#14354936 - 04/26/11 12:13 PM (12 years, 9 months ago) |
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I'd grab that pressurecooker back from 'em. Just swing by and be like "I left important stuff in the garage."
AA's are really expensive and high quality. Might as well pick that up and not bother with something smaller.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Is this normal for LC? [Re: Maverick]
#14354952 - 04/26/11 12:15 PM (12 years, 9 months ago) |
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Quote:
Maverick said: Don't get one of those 1970's pressurecookers.
One of mine is avocado green...
-------------------- You make it all possible. Doesn't it feel good?
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Re: Is this normal for LC? [Re: Maverick]
#14354956 - 04/26/11 12:16 PM (12 years, 9 months ago) |
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I think I will. My heart is racing even thinking about it, but I think it will be worth it. Well, I will let you know how this goes down. As long as I get there before my mom gets home I think I can avoid some drama lol.
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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Boozie
I like beer.



Registered: 02/18/10
Posts: 1,226
Loc: :ↄo⅃
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Re: Is this normal for LC? [Re: Maverick]
#14354974 - 04/26/11 12:20 PM (12 years, 9 months ago) |
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Quote:
Maverick said: I'd grab that pressurecooker back from 'em. Just swing by and be like "I left important stuff in the garage."
AA's are really expensive and high quality. Might as well pick that up and not bother with something smaller. 

I'd be over there in a heartbeat if I were you. Your stuff is still your stuff, even if you had a falling-out. A somewhat awkward conversation or two compared to a potential $75-100 purchase, there's no contest in my book. Especially since you'll never have to buy another PC again if you don't want to. And you never know, it may open up some lines of communication with them in the future.
-------------------- "After silence, that which comes nearest to expressing the inexpressible is music." - Aldous Huxley
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rustycobwebs
Green mold cultivator

Registered: 12/17/05
Posts: 799
Last seen: 8 years, 9 months
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Re: Is this normal for LC? [Re: Boozie]
#14355018 - 04/26/11 12:32 PM (12 years, 9 months ago) |
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hey....i like my 23-B 1973 presto....built like a tank.
Instead of coffee grinder and rice you could try the farm store for Max E Glo Rice bran meal. It's like rice with the hulls still on it then they have it already ground up for you! I've been using it with good results and less prep time.
If you do continue using LCs, do one test jar just to see if it's healthy or not....
-------------------- and let it be so Praise Psilocybe
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Well a little driving and an hour long awkward conversation later... SHE's BACK!!! I guess it was worth it. The conversation was little rough, but I got through it. It ended with: Mom:"I'm glad your going to church. If you keep going you will be a better person." Me: "huh?" Mom:"You're shirt says Lamb of God" Me: "Oh...ha"
P.S. This PC has a cord and plug on it and when I bought it I thought it was refereed to as an All-American Sterilizer. I don't have the manual anymore so I will be doing some searching online for how to use it, but is it still used on a stovetop or do I just have to fill it with water and plug it in?
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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BlackHat
Urban-Shaman



Registered: 03/07/11
Posts: 41
Last seen: 12 years, 6 months
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Re: Is this normal for LC? [Re: Sarfix]
#14356101 - 04/26/11 04:24 PM (12 years, 9 months ago) |
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i love Lamb Of God lmao
  
sucks about the bad lc but cool PC! looks big 
im tryin LC to right now but i should of read up a lot more i guess..
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