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Offlineygunz
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PCing ?
    #14353795 - 04/26/11 06:56 AM (12 years, 9 months ago)

Does pcing kill ALL contam (40 min @ 15 psi) Br/verm 1/2 pt jars


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OfflineDave Bowman
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Re: PCing ? [Re: ygunz]
    #14353809 - 04/26/11 07:01 AM (12 years, 9 months ago)

You should be OK but you may come across contams still.  The standard for PC'ing BRF cakes is at 15 psi for 60 minutes.

Although before I owned a PC... I used to just steam sterilize my jars... for 90 minutes without pressure... and I had 2 jars contaminate out of around 38.... but I'm sure I was an exception.

I'm sure you'll still have some jars do just fine but don't be surprised is some still get contams.

Next time try to shoot for 60 minutes @ 15 psi... and don't start the timer until you reach 15 psi... not just as soon as you start heating.


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Invisibleskyjohnny
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Re: PCing ? [Re: Dave Bowman]
    #14353922 - 04/26/11 07:38 AM (12 years, 9 months ago)

I agree with with synthettek.
To elaborate; When done properly, with proper preparation, PCing will kill all living organisms creating a sterile environment. Though killing most organisms can be enough to give the mushroom myc a fighting chance.
I personally never had very good luck cutting corners, I had very limited success until I went all out to serilize, now I have about 98% success. The other 2% is from when I overlook something or make a mistake.


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"Man is that he might have joy. Joy is the "true meaning" of life. May you all experience "true joy". -CJM


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Offlineygunz
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Re: PCing ? [Re: skyjohnny]
    #14353987 - 04/26/11 08:03 AM (12 years, 9 months ago)

thx guys , I went from min prep work (sterile) to bad contam


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Offlinear1es
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Re: PCing ? [Re: ygunz]
    #14359429 - 04/27/11 03:00 AM (12 years, 9 months ago)

i dont think pcing kills everything. i think it just kills the majority of baddies long enough for the myc to get a hold


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":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita


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Invisibleskyjohnny
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Re: PCing ? [Re: ar1es]
    #14359978 - 04/27/11 08:20 AM (12 years, 9 months ago)

Quote:

ar1es said:
i dont think pcing kills everything. i think it just kills the majority of baddies long enough for the myc to get a hold



  Some (if not all) ungerminated spores/endospores can withstand the heat, which is why we soak grains and such prior to PCing, to germinate these so that they may be killed in the PC. A properly sterilized and filtered grain jar can remain sterile and usable until it dries out. I had some sit for almost a year once. Opened one up to check for a smell, and they were all clean. They colonized perfectly.


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"Man is that he might have joy. Joy is the "true meaning" of life. May you all experience "true joy". -CJM


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