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Offlinezzantium2999
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fastest way to colonize ten pounds wild bird seed
    #14335067 - 04/22/11 08:04 PM (12 years, 9 months ago)

Let's say I have tten pounds of WBS. What would be the fastest way to colonize it all? So far I've been putting them in  pint jars, but that doesn't always work because sometimes a  few jars get contams, others just sit and do nothing, and some colonize. Whats the best way to colonize a huge batch of substrate?


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A good friend would bail you out of jail, but your best friend would be the one sitting next to you saying, "damn that was awesome".


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OfflineLarrythescaryrex
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Re: fastest way to colonize ten pounds wild bird seed [Re: zzantium2999]
    #14335084 - 04/22/11 08:08 PM (12 years, 9 months ago)

with a smaller batch of colonized substrate.


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RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011


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Offlinezzantium2999
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Re: fastest way to colonize ten pounds wild bird seed [Re: Larrythescaryrex]
    #14335107 - 04/22/11 08:13 PM (12 years, 9 months ago)

yea that part I know. I mean should I pour a little colonized substrate into 100 jars or should i throw it in a Bucket,t or a bag as one big substrate. I want to Make a huge substrate with thee least time. Right now all I have t inoculate with is. Colonized jars of brf sorry I wasn't being specific enough.


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A good friend would bail you out of jail, but your best friend would be the one sitting next to you saying, "damn that was awesome".


Edited by zzantium2999 (04/22/11 08:15 PM)


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OfflineEctius
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Re: fastest way to colonize ten pounds wild bird seed [Re: zzantium2999]
    #14335115 - 04/22/11 08:14 PM (12 years, 9 months ago)

Quote:

zzantium2999 said:
pint jars.
Whats the best way to colonize a huge batch of substrate?



Quart jars.

You could try filter-patch bags, but I wouldn't recommend it if contaminated jars are a regularity.


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Offlineskiddy
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Re: fastest way to colonize ten pounds wild bird seed [Re: Ectius]
    #14335130 - 04/22/11 08:16 PM (12 years, 9 months ago)

Use a LC


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PESH : Pinning
Transeski : colonizing
Orrisa : colonizing
Mex a : colonizing

You're not a mycologist just because you grow mushrooms.


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Offlinesteelmonkey
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Re: fastest way to colonize ten pounds wild bird seed [Re: zzantium2999]
    #14335131 - 04/22/11 08:16 PM (12 years, 9 months ago)

How much growing experience do you have? no offence but it doesn;t sound like much


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Offlinezzantium2999
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Re: fastest way to colonize ten pounds wild bird seed [Re: Ectius]
    #14335161 - 04/22/11 08:22 PM (12 years, 9 months ago)

not alot.  thats why I've only got 28 posts in. so anyways back to the question. Would it be better to colonize as one big substrate, or as many smaller ones? I would probably inoculate by using a uncontaminated jar of brf thats fully colonized


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A good friend would bail you out of jail, but your best friend would be the one sitting next to you saying, "damn that was awesome".


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Offlinesteelmonkey
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Re: fastest way to colonize ten pounds wild bird seed [Re: zzantium2999]
    #14335366 - 04/22/11 08:56 PM (12 years, 9 months ago)

It would take forever to colonize that amount of substrate with one jar of spawn and most likely it would contaminate before it fully colonised if your goal is to get a lot of fruits then you really need more spawn


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Offlinem_jacobs
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Re: fastest way to colonize ten pounds wild bird seed [Re: steelmonkey]
    #14335512 - 04/22/11 09:24 PM (12 years, 9 months ago)

If your currently having trouble with contams doing jars your problems are only going to get worse with contams doing one LARGE spawn.  The problem with doing a single LARGE tube or container all at once is that if you get ONE single contam your WHOLE setup is gone.

It is VERY VERY difficult to keep things completely sterile and contam free.  By breaking up the 10lbs of WBS into say 10 Quart jars you limit your risk of losing everything.

I'm assuming your starting with a MS syringe.  I would do 3 pint jars 1/2 filled with WBS with 4 inoculation points.  Depending on the viability of your spores it will take 3-14 days for them to show signs of growth.  Mine have been averaging 3-4 days room temp NO incubator.

Once you get growth bigger about size of a quarter on all 4 sides give them a shake.  Once they are 100% colonized do G2G transfer to your quart jars. 

I have 6 pint jars that were 100% from MS spore syringe exactly at 12 days.  From those I put about 3-4 big tablespoons into my quart jars and those finished off in 8 days.  Total of 20days.

My 1st run at doing straight quart jars and MS took 35 days.


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Where I got my supplies


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InvisibleDoc_T
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Re: fastest way to colonize ten pounds wild bird seed [Re: m_jacobs]
    #14336002 - 04/22/11 11:01 PM (12 years, 9 months ago)

^^^ that's the answer. :thumbup:


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You make it all possible. Doesn't it feel good?


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Invisiblefngbronco
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Re: fastest way to colonize ten pounds wild bird seed [Re: Doc_T]
    #14336357 - 04/23/11 12:23 AM (12 years, 9 months ago)

Y do u have 4 inoculation points on grains? With grains and ms you shoot and shake. 3 extra holes can introduce contaminants.

OP if you've had a hard time with the grains and have a colonized cake, fruit the cake and make spore prints. Then use the spore prints to make more cakes and a couple jars of grains until you get the grains down. Grains are tough to get down, but once you do you're golden! If you're trying to go bulk it'd take more than 1 cake unless you do like a 2.5qt mini, but even then I'd say fruit it and let the fruits mature for spore prints. If you can take aseptic spore prints you're heading in the right direction to making good grain jars


Best of luck!


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I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

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Offlinerustycobwebs
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Re: fastest way to colonize ten pounds wild bird seed [Re: fngbronco]
    #14336857 - 04/23/11 02:18 AM (12 years, 9 months ago)

If you're using multispore, I wouldn't shake them after shooting, I just use yes 1 injection port and kinda, shoot the CC or 2 of spore solution around in kind of a circle?

It seems to work for me... but I think shaking is a bad idea, might be dead wrong. I shake after injecting LC though, cuz that's already germinated.

You could break up colonized cakes and spawn that to the jars....


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and let it be so Praise Psilocybe


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Invisibleshroomiin
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Re: fastest way to colonize ten pounds wild bird seed [Re: rustycobwebs]
    #14337229 - 04/23/11 06:42 AM (12 years, 9 months ago)

quart jars man, do it right they should all colonize without contam..


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Offlinem_jacobs
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Re: fastest way to colonize ten pounds wild bird seed [Re: fngbronco]
    #14337495 - 04/23/11 08:54 AM (12 years, 9 months ago)

Quote:

fngbronco said:
Y do u have 4 inoculation points on grains? With grains and ms you shoot and shake. 3 extra holes can introduce contaminants.




His primary objective is speed on colonizing 10 lbs of WBS.
4 inoculation points with 1/4cc MS shots into pint or quart jar will colonize faster then 1 inoculation point with 1cc MS shot. Then he can do G2G or GLC to Quarts.

All inoculation holes are taped (or self healed) after inoculations.  Inoculation holes are NOT left opened or unfiltered when doing Grain.  When doing Grain you must have a GE HOLE that is filtered!  Inoculation points are left opened only if you are doing some type of CAKE tek with a verm layer.

Also you DO NOT shake after inoculation with MS shots. You only shake if your using any type of LC with live mycelium.  The reason is if you shake you make it harder for the initial mating process of germinated spores. 


Quote:

shroomiin said:
quart jars man, do it right they should all colonize without contam..



The reason I decided to do pint jars but only 1/3 to 1/2 full is it will become 100% colonized faster.  Once the tiny 1/3 pint jar is fully colonized he can do G2G or make a GLC and noc up quart jars.  You ever notice how quickly your jars go to 100% after you do the initial shake at 20-30%?  Its using the same idea.  You take already colonized grains and using them at MANY MANY different inoculation points inside of a quart jar.  **Again his primary goal was SPEED.


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Where I got my supplies


Edited by m_jacobs (04/23/11 09:03 AM)


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Invisiblefngbronco
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Re: fastest way to colonize ten pounds wild bird seed [Re: m_jacobs]
    #14337617 - 04/23/11 09:53 AM (12 years, 9 months ago)

You can put the needle in and spray the spores in a circular motion with a jar that's 1/2 full. That's why the 4 holes don't make sense to me


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I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek


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InvisibleFungal growth
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Re: fastest way to colonize ten pounds wild bird seed [Re: m_jacobs]
    #14337796 - 04/23/11 10:54 AM (12 years, 9 months ago)

Quote:

m_jacobs said:
The reason I decided to do pint jars but only 1/3 to 1/2 full is it will become 100% colonized faster.  Once the tiny 1/3 pint jar is fully colonized he can do G2G or make a GLC and noc up quart jars.  You ever notice how quickly your jars go to 100% after you do the initial shake at 20-30%?  Its using the same idea.  You take already colonized grains and using them at MANY MANY different inoculation points inside of a quart jar.  **Again his primary goal was SPEED.



good advice imo as i do that too. often when doing ms,
i will use half-pints (the regular mouth are better for this)
filled halfway. this will be colonized much faster than an
entire qt, and then i g2g to 2-4 qt jars per half pint jar.


Edited by Fungal growth (04/23/11 10:55 AM)


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Offlinem_jacobs
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Re: fastest way to colonize ten pounds wild bird seed [Re: fngbronco]
    #14337904 - 04/23/11 11:23 AM (12 years, 9 months ago)

Quote:

fngbronco said:
You can put the needle in and spray the spores in a circular motion with a jar that's 1/2 full. That's why the 4 holes don't make sense to me




This works perfectly well too. I have tried this method and the jars worked just as well as the 4 holes. 

It really breaks down to personal preference.  I do all my inoculations inside of a glove box.  So I just found it easier for me to squirt 1/4cc 4x then it was to try and do a single squirt while rotating the jar.  Also I don't like the idea of injecting through the GE hole.  That is just my own paranoia.


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Offlineafrosheen
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Re: fastest way to colonize ten pounds wild bird seed [Re: m_jacobs]
    #14338010 - 04/23/11 11:46 AM (12 years, 9 months ago)

No point in 4 inoculation holes with grains, even when doing MS. You shoot and shake regardless. Don't worry about the spores finding sex mates, they already did it in the syringe.


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InvisibleFooMan
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Re: fastest way to colonize ten pounds wild bird seed [Re: zzantium2999]
    #14338073 - 04/23/11 11:57 AM (12 years, 9 months ago)

Quote:

zzantium2999 said:
Let's say I have tten pounds of WBS. What would be the fastest way to colonize it all?




This is what you need: http://forums.mycotopia.net/spawning-teks/30494-tv-guide-colonizing-quart-jar-three-days.html#post467474


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Quick WBS Prep


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Invisiblefngbronco
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Re: fastest way to colonize ten pounds wild bird seed [Re: FooMan]
    #14338868 - 04/23/11 02:56 PM (12 years, 9 months ago)

Biggest issue with 4 holes is you're drawing and and exposing the needle to 4x the contaminants. I use a section of SFD for me GE, and a RTV'd hole for my needle so I know I'm sealed on the lid. It is all personal, but you open yourself up to failure (contamination) putting a needle in the same jar 4 times.


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek


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