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OfflineJayslay
Myco and Zymurgy
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Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Testing variables in PFtek and Rye 1/2s
    #14329974 - 04/21/11 08:49 PM (12 years, 9 months ago)

So after getting my buzz on for the long weekend, I am starting to devise a plan for some variable tests.  A dozen half pints, six for PFtek trials and six for Rye.  The variables proposed will be

1. Control jar (PF tek or basic rye tek)= F1
2. F1 + 10% spent coffee grounds 
3. F1 + 25% spent coffee grounds
4. F1 + Gypsum 7.5% bv    (additive test)
5. F1 + WBS (Soak n simr) 10%      (mixed substrate test)
6. F1 plus X?????

What should my final variable be, tested on Rye grains and PFtek.  I will document and photograph.


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Beer.


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Offlineafrosheen
9Lives the cat
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Registered: 03/06/10
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Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14330019 - 04/21/11 08:56 PM (12 years, 9 months ago)

Try making wbs flour. Take some unrinsed, straight out of the bag wbs, put it through the coffee grinder until it's fine, replace BRF in recipe. It's pretty good stuff.


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InvisibleDoc_T
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Folding@home Statistics
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Posts: 42,395
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Re: Testing variables in PFtek and Rye 1/2s [Re: afrosheen]
    #14330040 - 04/21/11 08:58 PM (12 years, 9 months ago)

So you're shooting spores?


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You make it all possible. Doesn't it feel good?


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OfflineJayslay
Myco and Zymurgy
Male


Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: Doc_T]
    #14330116 - 04/21/11 09:11 PM (12 years, 9 months ago)

I guess I didnt specify innoculant.  Will be a honey LC.


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Beer.


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OfflineJayslay
Myco and Zymurgy
Male


Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14330128 - 04/21/11 09:13 PM (12 years, 9 months ago)

Doc, that your girl in the avatar?  Makes me wanna start posting hot ones of my gal....  Kinda wondered about that since I been back at the Shroomery.  Haha


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Beer.


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Offlineafrosheen
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Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14330152 - 04/21/11 09:22 PM (12 years, 9 months ago)

Naw that's the lovely Boxxy.


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Invisiblewildernessjunkie
Reshitivest
I'm a teapot


Registered: 06/13/10
Posts: 8,118
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Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14330163 - 04/21/11 09:23 PM (12 years, 9 months ago)

How about the final jar with Yeast added?


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OfflineJayslay
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Registered: 03/22/11
Posts: 75
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Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: wildernessjunkie]
    #14330192 - 04/21/11 09:32 PM (12 years, 9 months ago)


I do have brewers yeast as well as baking yeast.  Whats your hypothesis?


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Beer.


Edited by Jayslay (04/23/11 05:34 PM)


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Offlinepslyke
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Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14330199 - 04/21/11 09:35 PM (12 years, 9 months ago)

Quote:

Jayslay said:
Doc, that your girl in the avatar?  Makes me wanna start posting hot ones of my gal....  Kinda wondered about that since I been back at the Shroomery.  Haha




I see absolutely no reason why you shouldn't share pics of your gal....


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"What appears impenetrable to us does exist, manifesting itself in the deepest wisdom and the most radiant beauty" Einstein

"The conservatives of 70 years ago would be outraged at what has come to pass. It embodies everything they took up arms for to defeat"Asante


:kratom:


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Invisiblewildernessjunkie
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Re: Testing variables in PFtek and Rye 1/2s [Re: pslyke]
    #14330618 - 04/21/11 11:06 PM (12 years, 9 months ago)

Yeast is sometimes added to both LCs and substrate with the idea that it provides nutritional benefits to the myc. You have the control jars, if growth is better/stronger/faster in the jars containing yeast, then that would confirm or deny the value of adding yeast.


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OfflineJayslay
Myco and Zymurgy
Male


Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: wildernessjunkie]
    #14339484 - 04/23/11 04:59 PM (12 years, 9 months ago)

Decided to do this in a smaller scale, in some extra pint jars I have.
-----------------------
1 Rye control: 16 hr soak w/tsp gypsum/pint, simmer to al dente, drain n strain dry

1 rye as above with 15% coffee ground, 5% gypsum, 5-10 verm

1 brf PF control 1:2:1 BRF:Verm:H20  (plus a small fraction more of verm to counter-act substrate compaction in pint jar) 

1 Mixed jar 60/30/10/5/5  BRF PFmix/Rye/Coffeegrounds/gyp/verm
---------------------------------------------------------------

  As usual, my sterile technique will be put to the test, as I WILL NOT be using a PC. 
The jars containing rye will have 20 extra minutes under steam boil in a canning pot. This is in addition to the full hour the PF base receives.
Columbian MH coffee grounds were taken fresh after a brew :wink:, and microwaved for a couple minutes before they was mixed with the substrates. 
Rye is organic, and the soak water was also the simmer water.

Jars are pints, lids are double rtv ports and a polyfill GE port 1/4".  MS PF, from a diluted proven syringe.  Will use 2cc, 1 per port.

Will be checking for colonization times, contaminations, and eventually analyzing fruiting speeds, and fruiting yields.  This test will also evaluate my rye technique, as today is my first!  I really wanted to see how things change between a more homogenous mixture and the mixed substrates.  Probably done before, but its a long weekend and I am hungry for some psilly science.

To tell you the truth, I am almost pushing it in terms of using the rye and coffee without a PC.  But i did give the rye some time to germinate some of its attached endospores, and I think the coffee grounds being fresh, and pre-micro'ed might up my chances. 
Oh and Ive upgraded my lid technique  :smile:


--------------------
Beer.


Edited by Jayslay (04/23/11 05:04 PM)


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InvisibleDoc_T
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Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14339503 - 04/23/11 05:03 PM (12 years, 9 months ago)

Not using a PC is not a test of your sterile technique. It's a test of your ability to comprehend sterile technique.
You can't have sterile technique if your materials are not sterile. Buy a PC, dude. Ten bucks.


--------------------
You make it all possible. Doesn't it feel good?


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OfflineJayslay
Myco and Zymurgy
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Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: Doc_T]
    #14339527 - 04/23/11 05:08 PM (12 years, 9 months ago)

I certainly will be, thanks for the advice.  I just finished several jars of WBS that did not contaminate at all, so I guess I may be getting cocky.  Thats why I only did a bit of Rye today instead of a full case.

Thanks for the Debby downer though  :wink:..........

My rye control serves the purpose of telling me whether my rye prep and method were effective, not whether they are recommended.  If it goes green then I will mail you a beer.

(You are right though, I can afford one and I should get one)


--------------------
Beer.


Edited by Jayslay (04/23/11 05:12 PM)


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InvisibleDoc_T
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Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14339553 - 04/23/11 05:12 PM (12 years, 9 months ago)

When you talk about the WBS, have you fruited that grow yet?
It ain't a success until it's successful, y'know?


--------------------
You make it all possible. Doesn't it feel good?


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OfflineJayslay
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Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: Doc_T]
    #14339661 - 04/23/11 05:28 PM (12 years, 9 months ago)

Well, to be honest no.  I have spawned it all and its incubating in three smaller batches.  I pastuerized spawn in quart jars in the same steam canner, using a new digital meat thermo with extended probe cord.  Held it at optimal (around 160-170) for a full hour.    I am feeling pretty good so far though because the volume of WBS that has been colonizing properly, with no contams...I will be honest further and say that I did have two of the original pints stall on me, my lids didnt allow enough GE. 

I guess when I do get some success I will come back and report.  Seems anything less is hater worthy. Thanks dude, I actually was starting to be fond of your intelligence.


--------------------
Beer.


Edited by Jayslay (04/23/11 05:36 PM)


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Offlineafrosheen
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Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14339732 - 04/23/11 05:41 PM (12 years, 9 months ago)

Quote:

I guess when I do get some success I will come back and report.  Seems anything less is hater worthy. Thanks dude, I actually was starting to be fond of your intelligence.




Now who's drinking the haterade? :rofl:


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OfflineJayslay
Myco and Zymurgy
Male


Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: afrosheen]
    #14339775 - 04/23/11 05:50 PM (12 years, 9 months ago)

Well haters are haters,.......................

It don't mean nothin :wink:


--------------------
Beer.


Edited by Jayslay (05/01/11 05:21 PM)


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OfflineJayslay
Myco and Zymurgy
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Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14384261 - 05/01/11 06:42 PM (12 years, 8 months ago)

Well, a week later and three of the four jars have the early growth of mycelium.  The only jar with no noticable growth of any kind is the rye control jar.
I suspect the diluted syringe to be the cause of the long period of germination.  I basically diluted a couple ccs " into just under twenty parts sterile water...  Won't be doing that anymore, but at the time it was a low cost alternative.
  The jar with the most points of growth is the PFtek control, as well as the enhanced BRF/Rye mix.  They are about equal. 
Will post pics in a week with growth between inoculation, one week and two weeks.


--------------------
Beer.


Edited by Jayslay (05/01/11 07:09 PM)


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InvisibleDoc_T
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Re: Testing variables in PFtek and Rye 1/2s [Re: Jayslay]
    #14384278 - 05/01/11 06:47 PM (12 years, 8 months ago)

So did you shoot spores into the jars after all? Not LC?


--------------------
You make it all possible. Doesn't it feel good?


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OfflineJayslay
Myco and Zymurgy
Male


Registered: 03/22/11
Posts: 75
Loc: Canada
Last seen: 7 years, 9 months
Re: Testing variables in PFtek and Rye 1/2s [Re: Doc_T]
    #14384363 - 05/01/11 07:11 PM (12 years, 8 months ago)

Ya I decided to use spores I had, I didnt see much growth in my LC.  I think I might have made my rye a little on the dry side too.


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Beer.


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