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Invisiblewildernessjunkie
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Cloneing Store Bought Mushrooms?
    #14323879 - 04/20/11 04:29 PM (12 years, 9 months ago)

The grocery stores where I am, have some nice King Oysters, and Shimejis for sale. Would there be any forseeable problems if I were to clone these to agar? Would this create problems later down the line, being that it would basically be a clone of a clone?

I could just buy a new culture, but if I can get these locally, that means I can spend my money on species that I cant buy in the store.


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Offlinetrout
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Re: Cloneing Store Bought Mushrooms? [Re: wildernessjunkie]
    #14323993 - 04/20/11 04:54 PM (12 years, 9 months ago)

It will work but you might be close to scenessence and threfore reduced vigor.  I have done it a few times with oysters and it worked fine but I didnt try to keep the cultures going very long. Give it a try and then start from spores if you can from your fruit. Good luck!!


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Invisibleskyjohnny
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Registered: 04/21/10
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Re: Cloneing Store Bought Mushrooms? [Re: trout]
    #14324182 - 04/20/11 05:45 PM (12 years, 9 months ago)

The mushrooms I find at the stores here seem to have myc growing near the base of the stem, I have considered trying the same, so I'll be taking notes.


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"Man is that he might have joy. Joy is the "true meaning" of life. May you all experience "true joy". -CJM


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Invisibleskyjohnny
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Re: Cloneing Store Bought Mushrooms? [Re: skyjohnny]
    #14337605 - 04/23/11 09:47 AM (12 years, 9 months ago)

After reading a lot of old threads, it appears that shittake clone fairly well. A. Bisporus, and oysters are often close to scenessence by the time they are in the store. Though I found one that used a piece of gill tissue from a store bought portobello to innoclate agar and it worked. It also fruited on straw/poo, with a pasteurized casing.
I am definitely gonna try this. I like a challenge.


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"Man is that he might have joy. Joy is the "true meaning" of life. May you all experience "true joy". -CJM


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OfflineMycelio
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Re: Cloneing Store Bought Mushrooms? [Re: skyjohnny]
    #14337721 - 04/23/11 10:33 AM (12 years, 9 months ago)

Just try. It is a cheap and simple way to get experience. I often cloned store bought mushrooms of all kinds including shimeji, oysters and A. bisporus and never encountered any trace of senescence. In case you want a fresh multispore culture of Agaricus species, using gill tissue is perfect, as it ensures reliable and fast spore germination. For me, cloning them directly on straw/poo in small jars works best.

Carsten


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OfflineCuriousGeorge83
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Registered: 02/21/09
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Re: Cloneing Store Bought Mushrooms? [Re: Mycelio]
    #14337958 - 04/23/11 11:35 AM (12 years, 9 months ago)

I just recently cloned a supermarket shimeji.  It was very slow on agar to start but finally (like over two weeks) I had a fully colonized plate.. I took some pieces of that plate to transfer to new plates and they colonized real fast.  Haven't put them on grain yet, but I plan on it at some point soon - then to sawdust to see if they still will fruit well.  I'm not getting my hopes up though..it was just an experiment.  It may fruit well, it may not...


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