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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag

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OfflineLightShedder
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Kobels agar b medium???
    #14320614 - 04/19/11 11:19 PM (12 years, 10 months ago)

I found "H. Kobel's agar b medium (et al. Helv chim acta 47:1052 1964)" being listed as a suitable agar medium for the storage of c. Paspali. Problem is, I can't find any articles describing what this is. I was hopin someone here might be able to direct me to some info on it.


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OfflineMolecularConcept
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Re: Kobels agar b medium??? [Re: LightShedder]
    #14321084 - 04/20/11 01:00 AM (12 years, 10 months ago)

The answer to your question is in the same sentance in the paper you got it from.. "containing beer wort and corn steep liquer" so I immagine its an agar recipe with those things in it... I don't know the concentration..


It can be stored in "human blood serum" at -22 -55.

Also

Storage at 4C on silica gel.  Tubes were filled 2/3 full with silica ge sterilized then .5ml submerged culture added. Submergen in .5ml sterile skim milk. Tube sealed with hot parifin and stored at 4C

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OfflineLightShedder
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Re: Kobels agar b medium??? [Re: MolecularConcept]
    #14321096 - 04/20/11 01:04 AM (12 years, 10 months ago)

I was thinking the beer wort /corn liquor thing couldn't be all there was to it. Maybe so. Those other methods may be employed but a simple agar media would be sweet. Hopefully it's just PDA with beer wort added. Probably like a tea spoon per cup of agar mix to start out. How do I know if it's adequate though until I go back to the culture 18 months later to transfer it?


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OfflineMolecularConcept
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Re: Kobels agar b medium??? [Re: LightShedder]
    #14321127 - 04/20/11 01:14 AM (12 years, 10 months ago)

Haha I don't know. They didn't say the storing mycelium under distilled water DIDN'T work. Worth a try

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