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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlinequebus
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Registered: 05/01/10
Posts: 678
Last seen: 2 months, 23 days
Injecting WBS thru Synthetic Filter Discs - fail
    #14319474 - 04/19/11 07:55 PM (12 years, 9 months ago)

I went to inject my WBS jars and had a problem with the Synthetic Filter Discs.  I was in the still air box, which is awkward to begin with,  and could not gauge the amount of thrust it would take to penetrate the disc (and then the RTV) - it would not give to the needle!  I was applying enough pressure that I was concerned about something going wrong, so I removed the lid - exposing the WBS momentarily and inoculated that way.  I did four quart jars like this.  Hopefully my box was clean enough that all will be well.

Am I missing something?


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OfflineDamion5050
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Registered: 05/01/08
Posts: 12,493
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Re: Injecting WBS thru Synthetic Filter Discs - fail [Re: quebus]
    #14319518 - 04/19/11 08:06 PM (12 years, 9 months ago)

Well normaly I don't inject through a filter disk anyways, and when I do inoculate grain jars I tend to do it with agar, so I have to open the lids anyways, but I do use a flow hood.


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Injecting WBS thru Synthetic Filter Discs - fail [Re: quebus]
    #14319531 - 04/19/11 08:10 PM (12 years, 9 months ago)

Never inject through a filter disk.  Once growers move to filter disks, they're assumed to have progressed beyond syringes.  Lift the lid in front of a flowhood and inoculate with an agar wedge or via grain to grain transfer.
RR


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Offlinequebus
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Registered: 05/01/10
Posts: 678
Last seen: 2 months, 23 days
Re: Injecting WBS thru Synthetic Filter Discs - fail [Re: RogerRabbit]
    #14319599 - 04/19/11 08:23 PM (12 years, 9 months ago)

Duh. Live and learn. Thanks.


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