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Offlinedr harry bush
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exposing mycelium to uvc lighting
    #14317374 - 04/19/11 01:14 PM (12 years, 9 months ago)

Maybe in not typing the correct keywords but i have found no real good info on this. ( iv found a lot of people asking stupid ass questions though )

i am going to experiment with exposing mycelium to uvc lighting for varies time and see what the outcome will be. The timing i am using is 5 seconds, 30 seconds, 1 minute, 5 minutes, 10 minutes, 20 minutes, 30 minutes and 1 hour. i highly doubt that the mycelium will survive after the 5 minute mark.
I will monitor the grow on agar and will try fruit these side by side.

i am using a Transkei iso. On MEA with a pinch of activated charcoal

i will post more pictures when this progresses 



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Offlineshopdropper
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14317411 - 04/19/11 01:20 PM (12 years, 9 months ago)

Awesome! Looking for mutations?


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: shopdropper]
    #14317465 - 04/19/11 01:30 PM (12 years, 9 months ago)

yes i am, but i would also like to know how long it would take to kill mycelium with uvc. as i mentioned i dont think they mycelium will past the 5 minute mark. this will also help with the next time i try this. i will know the time frame i have for uvc exposer.


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Offlineklik339
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14317842 - 04/19/11 02:37 PM (12 years, 9 months ago)

Definitely curious to see the outcome of this.


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: klik339]
    #14318082 - 04/19/11 03:24 PM (12 years, 9 months ago)

Is that just a black light? Or an actual UV lamp?


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: MolecularConcept]
    #14318203 - 04/19/11 03:46 PM (12 years, 9 months ago)

its a uv-c light. not one of those black lights they use to check notes.

Electrical Characteristics
Rated Lamp Wattage 15 W Technical Lamp Power 15.9 W Lamp Voltage 54 V Lamp Current 0.34 A Environmental Characteristics
Mercury (Hg) Content 2.0 mg UV-related Characteristics
UV-C Radiation 4.9 W Product Dimensions
Base Face to Base Face A 437.4 mm Insertion Length B 442.1 (min), 444.5 (max) mm Overall Length C 451.6 mm Diameter D 28 mm

as the myth goes pf use a black light that was on 24hours a day. i have tried this and i had no results, i was told i should try a uv-c bulb instead because it was the right wavelength.


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14318485 - 04/19/11 04:47 PM (12 years, 9 months ago)

254 nm yeah. I ws gonna get one of those for photo chemical reactions.. I found another way though. Still might be woth purchacing one now that ill be working with fungus again..


Make a glovebox with one of those in it. Turn it on for 10 mins prior to use? Kill all the biologicals in the box?  Might wanna try to mutate fungi too? :shrug: I'm interested in seeing your results!


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: MolecularConcept]
    #14322882 - 04/20/11 12:45 PM (12 years, 9 months ago)

I have had some surprising results today. Every one of the cultures are showing signs of growth. The culture that was exposed to one hour of UVC lighting is showing faster signs of growth to the other cultures. I am thinking I should expose them to the uvc lighting again?


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OfflineRogerRabbitM
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14322960 - 04/20/11 01:04 PM (12 years, 9 months ago)

We need to form an SPCM.

Society for Prevention of Cruelty to Mushrooms. :lol:
RR


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: RogerRabbit]
    #14323749 - 04/20/11 04:01 PM (12 years, 9 months ago)

Lol, unfortanlty sometimes it may seem crawl, but hopefully we will gain more knowledge from this experiment.
I was under the impression that uvc radiation would kill bacteria along with mycelium within the first 10 minutes? This does not seem to be the case. (well with the mycelium anyway)
I will expose these same cultures to the same amount of uvc light tomorrow and see if there is any difference to the growth on agar after 24 hours of the second exposure. I will then transfur to grain then fruit these.

:docbrown:


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Re: exposing mycelium to uvc lighting *DELETED* [Re: dr harry bush]
    #14324886 - 04/20/11 08:35 PM (12 years, 9 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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Re: exposing mycelium to uvc lighting [Re: Morelman]
    #14325911 - 04/21/11 12:34 AM (12 years, 9 months ago)

I wouldn't re expose the mycelium to light. If you have induced a frame shift mutation by exposing to UV light and causing pyrimidine dimers, if you re expose them to visible light they are able to correct or fix the mutation by the process of photoreactivation repair. This requires visible light in the blue spectrum I believe.

The fungi contain DNA repair enzymes (photolyases) so after you irradiate the hell out of them, you may want to keep them in near darkness or block the blue spectrum light when checking for growth.


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: Therian]
    #14326008 - 04/21/11 01:14 AM (12 years, 9 months ago)

That's awesome info you guys. UVC being blocked and the photorepair stuff. Good shit.


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Therian]
    #14326232 - 04/21/11 03:03 AM (12 years, 9 months ago)

thanks moleman i am aware of this and thanks for the info Therian.
would you then recommend exposing to the uvc lighting then keep in total darkness and check the culture under black light for any growth ?


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14331311 - 04/22/11 01:18 AM (12 years, 9 months ago)

That sounda acceptable. Photodeveloping darkroom red lights (40 watt) would be okay too.


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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14331703 - 04/22/11 04:21 AM (12 years, 9 months ago)

Why don't you just perform a simple web search and read how it is usually done? 30 minutes of investigation should save you from month of useless tries. You could at least irradiate germinating spores. Treating single celled organisms should raise your chances to see mutations enormously. In hyphal networks with hundredths of thousands of nuclei, damaged ones will be replaced by healthy ones immediately.

Carsten


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Mycelio]
    #14331810 - 04/22/11 05:35 AM (12 years, 9 months ago)

hi mycelio.

i have been researching the net for clues on how this should be carried out. i have come up with a total of two experiments that are close to this one. i am basing mine closely to an experiment that was used to mutate mold.

i was hoping to have the info here so others could learn from it and i prefer practical than just reading the theory.

if you read the first post i wanted to see how many minutes of uvc it takes to kill mycelium and mutants would be a bonus. i do know that there is greater chance that nothing will happen and that the mycelium will be repaired/replaced .... blah blah blah ...... but there is just that 1 percent chance that something many happen or we might just learn something from this and turn a myth into reality


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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14331819 - 04/22/11 05:41 AM (12 years, 9 months ago)

Quote:

if you read the first post i wanted to see how many minutes of uvc it takes to kill mycelium




Totally dependent on the radiation flux.
Please be careful with this, UV light causes cancer, mutations, blindness and death. In humans.


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Doc_T]
    #14331829 - 04/22/11 05:45 AM (12 years, 9 months ago)

thanks doc t.
i know this. the light is controlled from outside the clean room.
i have been burnt by uvc lighting before,nice sun burn in under 20 seconds and hurts like hell.


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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14331835 - 04/22/11 05:49 AM (12 years, 9 months ago)

And that 20 seconds increases your eventual chance of skin cancer or other cancer greatly.
Not to hassle you, just pointing out for those reading along.

There's a UV thread every few weeks. Nothing much seems to ever come of it.
Lots of risk, little apparent reward.


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Doc_T]
    #14331849 - 04/22/11 05:55 AM (12 years, 9 months ago)

yip no worries doc, i have seen a few discussions about uvc lighting here but seems like it’s from people that are looking for alternatives to flow hoods or glove boxes or thinking of a fast way to sterilize their glove boxes.


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OfflineMycelio
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14332504 - 04/22/11 09:45 AM (12 years, 9 months ago)

Hi again,

Quote:

dr harry bush said:
i have been researching the net for clues on how this should be carried out. i have come up with a total of two experiments that are close to this one. i am basing mine closely to an experiment that was used to mutate mold.



within a minute I can find several documented procedures, for example related to creating sporeless mutants in the genus pleurotus. I'm sure there is also something with P. cubensis. Perhaps you just try different search terms.


Quote:

dr harry bush said:
i was hoping to have the info here so others could learn from it and i prefer practical than just reading the theory.



Sure, that is nice, but reading will give you important hints, which you won't find here.


Quote:

dr harry bush said:
if you read the first post i wanted to see how many minutes of uvc it takes to kill mycelium and mutants would be a bonus. i do know that there is greater chance that nothing will happen and that the mycelium will be repaired/replaced .... blah blah blah ...... but there is just that 1 percent chance that something many happen or we might just learn something from this and turn a myth into reality



Like Doc_T wrote, that time depends on the actual intensity of your UV-C light and the distance to the mycelium.

I also share Doc_T's concerns, please be careful and protect your skin and eyes!

Carsten


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: Mycelio]
    #14335505 - 04/22/11 09:23 PM (12 years, 9 months ago)

I'm about to do some digging for some papers on on this if anyone finds one of particular interest how about sharing.

I knew UVC could cause burns and blindness. I did not know it could do so that quickly!  Holy crap! So thanks for that warning.

  Keep us updated. I know a there are a few people here interested in mutations


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Re: exposing mycelium to uvc lighting [Re: MolecularConcept]
    #14336348 - 04/23/11 12:20 AM (12 years, 9 months ago)

Quote:

MolecularConcept said:
I know a there are a few people here interested in mutations




I hope these people will remember that UV light will cause mutations in them.


Not the cool kind, either. :nonono:


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Re: exposing mycelium to uvc lighting [Re: Doc_T]
    #14336944 - 04/23/11 03:03 AM (12 years, 9 months ago)

lets hope so!

So do the exposures in a covered area and turn light on remotely!  Otherwise cover exposed skin and wear a really good pair of shades.


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DON'T TRY THIS AT HOME: no guarantees can be made about the accuracy of the information herein. The information dicussed in these posts is purely hypothetical, and for intelectual purposes only. Any similarity between internet chat and real life is pure coincidence.

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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: shopdropper]
    #14339049 - 04/23/11 03:29 PM (12 years, 9 months ago)

Thanks Mycelio iv been looking into a bit more information on the net.

Just an update on this experiment. All the petris where exposed to the same amount of light again while the petri lids where removed. +- 24 hours the dishes where checked under black light to check growth. There has been new growth on all on all the dishes the pertri at one hour shows good growth but much less than the petris exposed to minimum uvc times. The petri that was exposed to uvc for 5 minutes is showing the best growth. I will be taking pictures of the petris tomorrow evening and will post them to show you the growth.


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14343655 - 04/24/11 02:06 PM (12 years, 9 months ago)

here are the updated pictures of growth. these dishes are being held above my blacklight bulb.



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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14343692 - 04/24/11 02:15 PM (12 years, 9 months ago)

Awesome Work DR, This is an amazing experiment :smile:


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Amphiprion]
    #14596477 - 06/11/11 02:59 PM (12 years, 7 months ago)

ok just an update on the progress of this grow. i currently have tk uvc 5 min in 6 jars of grain. i should be spawning this to ccv in about 7 days.

i will post pictures very soon.

peace


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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14761737 - 07/13/11 10:56 AM (12 years, 6 months ago)

good evening everyone

here is a little update on the progress of this experiment.
i spawned my grain to 5% coffee, 10% gypsum, 45% coir, 40% verm. i sealed up the mono for 3 days then exposed it to fruiting conditions, 7 days later i had pins and this is the 3rd day into fruiting.

i have not noticed any mutant as yet.

i will print a few of theses and grow them to see if there are any mutants down the line.



peace


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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14762875 - 07/13/11 02:19 PM (12 years, 6 months ago)

So stringy... What strain?


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Re: exposing mycelium to uvc lighting [Re: Toofpaste]
    #14765219 - 07/13/11 10:14 PM (12 years, 6 months ago)

High CO2?

Looks like you're in need of even higher exposure. Good work on the experiment btw! :thumbup:


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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #25465705 - 09/16/18 08:55 PM (5 years, 4 months ago)

Well wouldnt this help to keep trich and other contamination from happening? Or at least prevent it to keep your cakes longer? Im experimenting with perpetual colonies. Keeping them alive and I was curious on how well they faired against the light. I know they use these to completely sanitize everything so Im curous to see if this will keep a colony healthy past a few fruitings.


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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #25466360 - 09/16/18 08:55 PM (5 years, 4 months ago)

This thread has been closed.

Reason:
7 years old and no it would probably help trich. As uvc doesn't do much but damage mycelium the faster growing species like trich would likely have an easier time and run more rampantly over your cubes.


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