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Offlinedr harry bush
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exposing mycelium to uvc lighting
    #14317374 - 04/19/11 01:14 PM (12 years, 10 months ago)

Maybe in not typing the correct keywords but i have found no real good info on this. ( iv found a lot of people asking stupid ass questions though )

i am going to experiment with exposing mycelium to uvc lighting for varies time and see what the outcome will be. The timing i am using is 5 seconds, 30 seconds, 1 minute, 5 minutes, 10 minutes, 20 minutes, 30 minutes and 1 hour. i highly doubt that the mycelium will survive after the 5 minute mark.
I will monitor the grow on agar and will try fruit these side by side.

i am using a Transkei iso. On MEA with a pinch of activated charcoal

i will post more pictures when this progresses 



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Offlineshopdropper
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14317411 - 04/19/11 01:20 PM (12 years, 10 months ago)

Awesome! Looking for mutations?


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: shopdropper]
    #14317465 - 04/19/11 01:30 PM (12 years, 10 months ago)

yes i am, but i would also like to know how long it would take to kill mycelium with uvc. as i mentioned i dont think they mycelium will past the 5 minute mark. this will also help with the next time i try this. i will know the time frame i have for uvc exposer.


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Offlineklik339
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14317842 - 04/19/11 02:37 PM (12 years, 10 months ago)

Definitely curious to see the outcome of this.


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: klik339]
    #14318082 - 04/19/11 03:24 PM (12 years, 10 months ago)

Is that just a black light? Or an actual UV lamp?

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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: MolecularConcept]
    #14318203 - 04/19/11 03:46 PM (12 years, 10 months ago)

its a uv-c light. not one of those black lights they use to check notes.

Electrical Characteristics
Rated Lamp Wattage 15 W Technical Lamp Power 15.9 W Lamp Voltage 54 V Lamp Current 0.34 A Environmental Characteristics
Mercury (Hg) Content 2.0 mg UV-related Characteristics
UV-C Radiation 4.9 W Product Dimensions
Base Face to Base Face A 437.4 mm Insertion Length B 442.1 (min), 444.5 (max) mm Overall Length C 451.6 mm Diameter D 28 mm

as the myth goes pf use a black light that was on 24hours a day. i have tried this and i had no results, i was told i should try a uv-c bulb instead because it was the right wavelength.


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14318485 - 04/19/11 04:47 PM (12 years, 10 months ago)

254 nm yeah. I ws gonna get one of those for photo chemical reactions.. I found another way though. Still might be woth purchacing one now that ill be working with fungus again..


Make a glovebox with one of those in it. Turn it on for 10 mins prior to use? Kill all the biologicals in the box?  Might wanna try to mutate fungi too? :shrug: I'm interested in seeing your results!

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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: MolecularConcept]
    #14322882 - 04/20/11 12:45 PM (12 years, 10 months ago)

I have had some surprising results today. Every one of the cultures are showing signs of growth. The culture that was exposed to one hour of UVC lighting is showing faster signs of growth to the other cultures. I am thinking I should expose them to the uvc lighting again?


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OfflineRogerRabbitM
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14322960 - 04/20/11 01:04 PM (12 years, 10 months ago)

We need to form an SPCM.

Society for Prevention of Cruelty to Mushrooms. :lol:
RR


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: RogerRabbit]
    #14323749 - 04/20/11 04:01 PM (12 years, 10 months ago)

Lol, unfortanlty sometimes it may seem crawl, but hopefully we will gain more knowledge from this experiment.
I was under the impression that uvc radiation would kill bacteria along with mycelium within the first 10 minutes? This does not seem to be the case. (well with the mycelium anyway)
I will expose these same cultures to the same amount of uvc light tomorrow and see if there is any difference to the growth on agar after 24 hours of the second exposure. I will then transfur to grain then fruit these.

:docbrown:


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InvisibleMorelman
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Re: exposing mycelium to uvc lighting *DELETED* [Re: dr harry bush]
    #14324886 - 04/20/11 08:35 PM (12 years, 10 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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InvisibleTherian
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Re: exposing mycelium to uvc lighting [Re: Morelman]
    #14325911 - 04/21/11 12:34 AM (12 years, 10 months ago)

I wouldn't re expose the mycelium to light. If you have induced a frame shift mutation by exposing to UV light and causing pyrimidine dimers, if you re expose them to visible light they are able to correct or fix the mutation by the process of photoreactivation repair. This requires visible light in the blue spectrum I believe.

The fungi contain DNA repair enzymes (photolyases) so after you irradiate the hell out of them, you may want to keep them in near darkness or block the blue spectrum light when checking for growth.

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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: Therian]
    #14326008 - 04/21/11 01:14 AM (12 years, 10 months ago)

That's awesome info you guys. UVC being blocked and the photorepair stuff. Good shit.

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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Therian]
    #14326232 - 04/21/11 03:03 AM (12 years, 10 months ago)

thanks moleman i am aware of this and thanks for the info Therian.
would you then recommend exposing to the uvc lighting then keep in total darkness and check the culture under black light for any growth ?


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14331311 - 04/22/11 01:18 AM (12 years, 10 months ago)

That sounda acceptable. Photodeveloping darkroom red lights (40 watt) would be okay too.

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OfflineMycelio
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14331703 - 04/22/11 04:21 AM (12 years, 10 months ago)

Why don't you just perform a simple web search and read how it is usually done? 30 minutes of investigation should save you from month of useless tries. You could at least irradiate germinating spores. Treating single celled organisms should raise your chances to see mutations enormously. In hyphal networks with hundredths of thousands of nuclei, damaged ones will be replaced by healthy ones immediately.

Carsten

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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Mycelio]
    #14331810 - 04/22/11 05:35 AM (12 years, 10 months ago)

hi mycelio.

i have been researching the net for clues on how this should be carried out. i have come up with a total of two experiments that are close to this one. i am basing mine closely to an experiment that was used to mutate mold.

i was hoping to have the info here so others could learn from it and i prefer practical than just reading the theory.

if you read the first post i wanted to see how many minutes of uvc it takes to kill mycelium and mutants would be a bonus. i do know that there is greater chance that nothing will happen and that the mycelium will be repaired/replaced .... blah blah blah ...... but there is just that 1 percent chance that something many happen or we might just learn something from this and turn a myth into reality


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InvisibleDoc_T
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14331819 - 04/22/11 05:41 AM (12 years, 10 months ago)

Quote:

if you read the first post i wanted to see how many minutes of uvc it takes to kill mycelium




Totally dependent on the radiation flux.
Please be careful with this, UV light causes cancer, mutations, blindness and death. In humans.


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Doc_T]
    #14331829 - 04/22/11 05:45 AM (12 years, 10 months ago)

thanks doc t.
i know this. the light is controlled from outside the clean room.
i have been burnt by uvc lighting before,nice sun burn in under 20 seconds and hurts like hell.


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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14331835 - 04/22/11 05:49 AM (12 years, 10 months ago)

And that 20 seconds increases your eventual chance of skin cancer or other cancer greatly.
Not to hassle you, just pointing out for those reading along.

There's a UV thread every few weeks. Nothing much seems to ever come of it.
Lots of risk, little apparent reward.


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