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deadmandave
Slime


Registered: 02/16/10
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How often do you change your Agar Recipe?
#14311954 - 04/18/11 01:13 PM (12 years, 9 months ago) |
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I know its beneficial to change up an agar recipe so that the mycelium gets a change of pace and doesnt get too acclimated to one food source, but how often should the recipe be changed? Should it be everytime that a transfer is made it is onto a new agar recipe?
One other question is what are some easy recipes that you like to use? I have only tried malt extract yeast agar (MEYA). I have some dextrose that i got from a brewers supply, can i substitute the yeast with dextrose as a different food source?
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: How often do you change your Agar Recipe? [Re: deadmandave]
#14311973 - 04/18/11 01:16 PM (12 years, 9 months ago) |
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what you're talking about is probably called culture senescence and I think I've read it varies from specie to specie but is a number around 120 transfers.
so your agar recipe is safe for one hundred transfers at least I'd say, which is more than enough for most of us
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cyantific
Trusted Masturbator


Registered: 10/11/09
Posts: 1,323
Loc: Shakashuri Island
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Re: How often do you change your Agar Recipe? [Re: deadmandave]
#14311976 - 04/18/11 01:17 PM (12 years, 9 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/7081785#7081785
easiest and cheapest tek ever and the one i use ... i never switch up and never have a problem with growth ...
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Stains Blue
Stranger

Registered: 01/17/11
Posts: 963
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Re: How often do you change your Agar Recipe? [Re: deadmandave]
#14312009 - 04/18/11 01:22 PM (12 years, 9 months ago) |
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I change mine every transfer. Not because its the best. But because it is easy for me.
I have a bottle that holds enough agar for 20 plates. and each sleeve has 20 plates so I simple mix up a different recipe each time i pour some plates.
I only use 2 recipes
PD(Y)A & ME(Y)A
some times i do not use the yeast It depends if I have any on hand or not. And I have had good success doing this.
Some people don't change there recipes and some people do. you will hear different experiences on what to do and what not to.
I find it beneficial to change the recipe as to how often to change it... I don't know
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higgledy-piggledy
Registered: 08/24/10
Posts: 966
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Re: How often do you change your Agar Recipe? [Re: cc2]
#14312019 - 04/18/11 01:24 PM (12 years, 9 months ago) |
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Quote:
cc2 said: what you're talking about is probably called culture senescence and I think I've read it varies from specie to specie but is a number around 120 transfers.
so your agar recipe is safe for one hundred transfers at least I'd say, which is more than enough for most of us 
it would be save for a thousand of transfers.
if you were a hungry mycelium, would you refuse food one gives to you, if nothing else is available?
the mycelium wont get sick of eating the same over and over again.
cubensis mycelium is not a gourmet; it will eat nearly everything you serve.
finicky mycelium? not in my house!
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Quote:
higgledy-piggledy said:
it would be save for a thousand of transfers.
if you were a hungry mycelium, would you refuse food one gives to you, if nothing else is available?
the mycelium wont get sick of eating the same over and over again.
cubensis mycelium is not a gourmet; it will eat nearly everything you serve.
finicky mycelium? not in my house! 
grammar
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Perun
Mahapralaya...



Registered: 11/11/10
Posts: 585
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I change it every time...cuz i am lazy bastard and i am eyeballing everything!!! LOL
-------------------- First: Then:
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RogerRabbit
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Registered: 03/26/03
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Re: How often do you change your Agar Recipe? [Re: deadmandave]
#14312299 - 04/18/11 02:03 PM (12 years, 9 months ago) |
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Quote:
deadmandave said: I know its beneficial to change up an agar recipe so that the mycelium gets a change of pace and doesnt get too acclimated to one food source, but how often should the recipe be changed?
That's only a theory and not one I subscribe to.
I've had all of my cultures on MEA for well over a decade, and many of those were more than ten years old when I got them. I've not noticed one whit of deterioration in performance. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Damion5050
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Re: How often do you change your Agar Recipe? [Re: RogerRabbit]
#14312663 - 04/18/11 02:56 PM (12 years, 9 months ago) |
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All I use is the same PDA recipe for about 3 years now..
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hamloaf
Loaf of Fam.


Registered: 12/23/09
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Loc: Oklahoma.
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Re: How often do you change your Agar Recipe? [Re: deadmandave]
#14312722 - 04/18/11 03:03 PM (12 years, 9 months ago) |
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Quote:
One other question is what are some easy recipes that you like to use? I have only tried malt extract yeast agar (MEYA). I have some dextrose that i got from a brewers supply, can i substitute the yeast with dextrose as a different food source?
MEA is the easiest agar recipe available, IMHO and is what I use. Sometimes with the addition of mycological peptone or brewers yeast at .5 of a gram every 500ml of water. MEA is just 10 grams of LME and 9 grams of powdered agar agar per every 500ml of water.
I wouldn't substitute brewers yeast with dextrose. Dextrose is a pure form of sugar and brewers yeast is yeast. I'v also never read of an agar recipe calling for dextrose. I'd just keep the brewers yeast for use with agar and the dextrose for use with LC's.
Edited by liquidmyce (04/18/11 08:51 PM)
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Stains Blue
Stranger

Registered: 01/17/11
Posts: 963
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Re: How often do you change your Agar Recipe? [Re: RogerRabbit]
#14314395 - 04/18/11 10:29 PM (12 years, 9 months ago) |
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Quote:
RogerRabbit said: That's only a theory and not one I subscribe to.
I've had all of my cultures on MEA for well over a decade, and many of those were more than ten years old when I got them. I've not noticed one whit of deterioration in performance. RR
Hmmm I think RR just made my agar life a bit easier.
MEA it is for me. for now on! thats awesome!
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deadmandave
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Registered: 02/16/10
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Re: How often do you change your Agar Recipe? [Re: Stains Blue]
#14317036 - 04/19/11 12:13 PM (12 years, 9 months ago) |
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Awesome! thanks for all the responses I will just use a simple recipe from now on.
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