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OfflineBelief
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Registered: 03/14/11
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Bah Multi-spore, Best way to start?
    #14309856 - 04/18/11 12:11 AM (12 years, 10 months ago)

Let me just say I hate starting new.  Its a huge PITA but is necessary if you want to create and keep the best strain possible.  How do you guys get started?


I usually go agar>>blind isolation 1-2 times>>agar to grain>>bulk sub
or
MS LC >>grains>>bulk sub + pray

I haven't really found a method that has truly really come to my liking.  I usually do agar with prints and LC with sponsor syringes.



any input would be splendid :smile:  I would really love a better method of doing this.

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InvisibleUnnamedGrower
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Registered: 04/17/11
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Re: Bah Multi-spore, Best way to start? [Re: Belief]
    #14309867 - 04/18/11 12:14 AM (12 years, 10 months ago)

Why not just grow out the MS and clone the best specimen?  Thats what I do with great results

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OfflineCaptainAhab


Registered: 12/25/09
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Re: Bah Multi-spore, Best way to start? *DELETED* [Re: UnnamedGrower]
    #14310097 - 04/18/11 01:26 AM (12 years, 10 months ago)

Post deleted by RogerRabbit

Reason for deletion: Off topic post deleted.



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InvisibleUnnamedGrower
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Re: Bah Multi-spore, Best way to start? *DELETED* [Re: CaptainAhab]
    #14310117 - 04/18/11 01:32 AM (12 years, 10 months ago)

Post deleted by RogerRabbit

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OfflineCaptainAhab


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Re: Bah Multi-spore, Best way to start? *DELETED* [Re: UnnamedGrower]
    #14310176 - 04/18/11 01:51 AM (12 years, 10 months ago)

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InvisibleUnnamedGrower
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Re: Bah Multi-spore, Best way to start? *DELETED* [Re: CaptainAhab]
    #14310182 - 04/18/11 01:52 AM (12 years, 10 months ago)

Post deleted by RogerRabbit

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OfflineCaptainAhab


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Re: Bah Multi-spore, Best way to start? *DELETED* [Re: UnnamedGrower]
    #14310206 - 04/18/11 02:03 AM (12 years, 10 months ago)

Post deleted by RogerRabbit

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InvisibleUnnamedGrower
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Re: Bah Multi-spore, Best way to start? *DELETED* [Re: CaptainAhab]
    #14310216 - 04/18/11 02:06 AM (12 years, 10 months ago)

Post deleted by RogerRabbit

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OfflineBelief
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Re: Bah Multi-spore, Best way to start? [Re: UnnamedGrower]
    #14310234 - 04/18/11 02:12 AM (12 years, 10 months ago)

:frown: seven posts and I was hoping for an answer.


MS takes so long to colonize with straight up grains :/

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InvisibleUnnamedGrower
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Re: Bah Multi-spore, Best way to start? [Re: Belief]
    #14310240 - 04/18/11 02:15 AM (12 years, 10 months ago)

Clone is one of the best ways.  With your blind isolation until you test the iso out you have no clu how its going to grow.  You clone you know exactlly how its gonna perform:shrug:  Cloning is easy but its not something you can do to start you need to do an MS grow to get the fruit to clone from.  This IMHO is much better than blind iso's and having to test them all out.

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OfflineCaptainAhab


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Re: Bah Multi-spore, Best way to start? *DELETED* [Re: UnnamedGrower]
    #14310244 - 04/18/11 02:17 AM (12 years, 10 months ago)

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InvisibleUnnamedGrower
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Re: Bah Multi-spore, Best way to start? *DELETED* [Re: CaptainAhab]
    #14310248 - 04/18/11 02:20 AM (12 years, 10 months ago)

Post deleted by RogerRabbit

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OfflineBelief
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Re: Bah Multi-spore, Best way to start? [Re: UnnamedGrower]
    #14310254 - 04/18/11 02:23 AM (12 years, 10 months ago)

QQ thread ninjas.


MS to grains then g2g?

or MS a few jars then wait it out to spawn?

Edited by Belief (04/18/11 02:27 AM)

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InvisibleSilvioDante
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Registered: 04/10/11
Posts: 186
Re: Bah Multi-spore, Best way to start? [Re: Belief]
    #14310289 - 04/18/11 02:36 AM (12 years, 10 months ago)

Quote:

UnnamedGrower said:
You have some poo stabbing ass pirate as your avatar and your trying to step to me about having alex fr a clockwork orange?  Are you retarded? 

Honestly tho id have a battle of wits with you but your unarmed




lay off dustin hoffman
Quote:

Belief said:
MS takes so long to colonize with straight up grains :/




not for me. what kind of grains are you using? tek etc?

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OfflinePrimal Call
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Re: Bah Multi-spore, Best way to start? [Re: Belief]
    #14310291 - 04/18/11 02:37 AM (12 years, 10 months ago)

either, but if you expand, use GLC IMO. (unless you're working with a flowhood, then I envy you.) then fruit > agar clone > isolate > test!

:peace:


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OfflineBelief
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Re: Bah Multi-spore, Best way to start? [Re: Primal Call]
    #14310303 - 04/18/11 02:44 AM (12 years, 10 months ago)

24x24 flowhood :laugh:

The main issue i guess would be the "testing".

I've never tried cakes.  My friend dragged me right into bulk.  It seems like smaller would be better for MS trials. 

Cakes or bulk shoeboxes?

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InvisibleUnnamedGrower
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Re: Bah Multi-spore, Best way to start? [Re: Belief]
    #14310308 - 04/18/11 02:46 AM (12 years, 10 months ago)

cakes are great for testing iso's

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OfflineBelief
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Registered: 03/14/11
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Last seen: 8 years, 10 days
Re: Bah Multi-spore, Best way to start? [Re: UnnamedGrower]
    #14310368 - 04/18/11 03:22 AM (12 years, 10 months ago)

It seems the best method is to MS inoculate a bunch.  If i were to MS to cakes, and clone an isolation from those- would they preform the same on a larger substrate with different substrate?  I think cakes would be the wrong approach, but I could be wrong cakes offer the ability to test a lot in a small space

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InvisibleUnnamedGrower
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Re: Bah Multi-spore, Best way to start? [Re: Belief]
    #14310369 - 04/18/11 03:23 AM (12 years, 10 months ago)

Quote:

Belief said:
It seems the best method is to MS inoculate a bunch.  If i were to MS to cakes, and clone an isolation from those- would they preform the same on a larger substrate with different substrate?  I think cakes would be the wrong approach, but I could be wrong cakes offer the ability to test a lot in a small space



The same or better

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OfflineBelief
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Registered: 03/14/11
Posts: 87
Last seen: 8 years, 10 days
Re: Bah Multi-spore, Best way to start? [Re: UnnamedGrower]
    #14310374 - 04/18/11 03:26 AM (12 years, 10 months ago)

I've made bulk blocks with spawn bags.  and I'd say that a rice bran substrate differs greatly from a soil/manure substrate.

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