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OfflineEgoDeath0290
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Registered: 04/03/11
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8 Days After My First PF Tek Attempt (Pic)
    #14308818 - 04/17/11 08:52 PM (12 years, 9 months ago)

I inoculated 5 PF style jars on last Saturday, and they have been incubating now for 8 days. I followed the Let's Grow Mushrooms video guide, and used a multi-spore syringe obtained from The Hawk's Eye (a Shroomery sponsor). The brown rice flower is organic, and the vermiculite is Sta-Green brand. I used 1 part BRF, 1 part distilled Water, and 2 parts Verm just like the video suggests. I inoculated my jars in a still-air box, flame sterilizing in between jars. I first checked them after 4 days, finding nothing, but didn't think anything of it because this is normal. I took a peek at them again tonight, and still see no progression. However, I am discouraged more so by the grey-ish areas (I wouldn't call them spots, as they don't seem that much different than the rest of the sub) located at the inoculation sites. I was concerned that the sub may have been too dry in the first place, and so I figured at first they were just spots where the soil was more hydrated. It is worth mentioning that all 5 jars look this way.

I want some opinions from those more experienced. I wouldn't be posting if I hadn't already looked at many pictures of contaminated jars, only to find that none of them really resemble mine (at least not to me). I decided to empty a jar outside to see if I could detect any scent. There was a noticeable one, but it wasn't what I would consider offensive. It just smelled to me like potent soil. Kind of like first entering the home and garden center at a Home Depot. Here is a picture of my cakes. Notice that there is absolutely NO mycelium growth after 8 days. Resources for me are not a problem and I've already sort of started over, using another syringe from the same batch of B+. I've quarantined these 5 cakes, and placed them somewhere else outside of the incubator (which is worth mentioning, is a closed box kept at 80 degrees F using indirect heated airflow.) Are they worth keeping, just to see if something happens?

I have two Penis Envy syringes as well, but the B+ was supposed to be the easy one!

Cakes


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SWIM made me say/do/post it.


Edited by EgoDeath0290 (04/17/11 08:58 PM)


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OfflineBargainBab
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Registered: 04/04/11
Posts: 1,178
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Re: 8 Days After My First PF Tek Attempt [Re: EgoDeath0290]
    #14308874 - 04/17/11 08:58 PM (12 years, 9 months ago)

How moist was the soil you dumped?


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Oh wow, GOOD Nyborg!


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OfflineEgoDeath0290
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Registered: 04/03/11
Posts: 103
Loc: Great American Desert
Last seen: 12 years, 7 months
Re: 8 Days After My First PF Tek Attempt [Re: BargainBab]
    #14308911 - 04/17/11 09:02 PM (12 years, 9 months ago)

It came out as one solid clump, but broke apart pretty easy, I have a pic of that as well, just before I buried it.



Not very good, but you can see it is a little darker than normal dirt. When I fingered it, some dirt stuck to my fingers. I hope this is an accurate description.


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OfflineBargainBab
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Registered: 04/04/11
Posts: 1,178
Loc: Callisto
Last seen: 10 years, 2 months
Re: 8 Days After My First PF Tek Attempt [Re: EgoDeath0290]
    #14308972 - 04/17/11 09:10 PM (12 years, 9 months ago)

Sounds a bit dry, it should be wet enough that you can squeeze water out of it before PCing or steaming, and firm moist once it's sanitary


--------------------
Oh wow, GOOD Nyborg!


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OfflineEgoDeath0290
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Male

Registered: 04/03/11
Posts: 103
Loc: Great American Desert
Last seen: 12 years, 7 months
Re: 8 Days After My First PF Tek Attempt [Re: BargainBab]
    #14311940 - 04/18/11 01:10 PM (12 years, 9 months ago)

Could the type/variety of Verm I'm using be retaining more water than most, thereby requiring a slight alteration of the water content? The verm is very fine, like silt. Perhaps the greater surface area means I should use more like 1.25 parts water to 1 part BRF and 2 parts Verm.

What ratios do you guys use? It seems like most just stick with RR's suggestions, but you're right, my cakes seemed a little dry. Especially at the edges toughing the glass.


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SWIM made me say/do/post it.


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