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Offlinem_jacobs
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Registered: 03/11/11
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How to identify mono-culture (isolate) on agar?
    #14306671 - 04/17/11 02:19 PM (12 years, 9 months ago)

Hey everyone.  I tried doing some searches, and there are a few threads with people asking "Is this a mono-culture"  "Is this an isolate"?  The replys are often yes, no, you have sectors at X o'clock.

I was wondering if someone could write out, or show some clear examples of what to look for?

I'm current understanding of a mono-culture or isolate is to look for a nice perfectly round circle of mycelium. 

Here are two photos of my current attempt at Isolation. 

Petri dish "A"


Petri dish "B"


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OfflineRogerRabbitM
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Re: How to identify mono-culture (isolate) on agar? [Re: m_jacobs]
    #14306787 - 04/17/11 02:39 PM (12 years, 9 months ago)

Both look like mold.  What species are you intending to grow?
RR


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Offlinem_jacobs
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Re: How to identify mono-culture (isolate) on agar? [Re: RogerRabbit]
    #14307087 - 04/17/11 03:31 PM (12 years, 9 months ago)

Hmm I hadn't considered I might have isolated mold!  Hahaha that would be really funny and sad at the same time.

It is suppose to be P. cube "Cambodian"

The two chunks were taken from this MS dish.



What leads you to believe its mold?

and Thank you in advance for any help / replys.  greatly appreciated.


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Invisiblehiggledy-piggledy
Registered: 08/24/10
Posts: 966
Re: How to identify mono-culture (isolate) on agar? [Re: m_jacobs]
    #14309175 - 04/17/11 09:43 PM (12 years, 9 months ago)

Quote:

m_jacobs said:
Hmm I hadn't considered I might have isolated mold!



no problem dude; thats what agar is for.

not for isolating molds :tongue:, but to begin to separate the mold from the actual mycelium.

Quote:

m_jacobs said:
What leads you to believe its mold?



its cottony appearance...

but you can definitely see something like mycelial strands in the center of the second pic (dish b).


start seperating and isolating.

:goodluck:


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