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North Spore Shop: Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineLarrythescaryrex
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agar wedge sizing frustration.
    #14298201 - 04/15/11 09:46 PM (12 years, 9 months ago)

What is the approperiate size for an agar wedge for a grain jar. I've been looking but I can't find actual dimenisions...


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OfflineBargainBab
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Re: agar wedge sizing frustration. [Re: Larrythescaryrex]
    #14298260 - 04/15/11 10:00 PM (12 years, 9 months ago)

About the size of your index nail should be more than enough :smile:


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OfflineLarrythescaryrex
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Re: agar wedge sizing frustration. [Re: BargainBab]
    #14298317 - 04/15/11 10:11 PM (12 years, 9 months ago)

thanks!


--------------------
RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011


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OfflineRogerRabbitM
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Re: agar wedge sizing frustration. [Re: Larrythescaryrex]
    #14298581 - 04/15/11 11:09 PM (12 years, 9 months ago)

A fingernail is about right.  I use 1/3 petri dish for a large spawn bag which holds five to six quarts.
RR


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InvisibleFractalXplora
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Re: agar wedge sizing frustration. [Re: RogerRabbit]
    #14298684 - 04/15/11 11:29 PM (12 years, 9 months ago)

I sometimes use 2 little wedges just to get a couple of inoculation points in my jars to speed things along, but I'm impatient!


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Offlinehamloaf
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Re: agar wedge sizing frustration. [Re: FractalXplora]
    #14299124 - 04/16/11 12:57 AM (12 years, 9 months ago)

The more wedges you inoculate a sterile substrate with, the faster colonization'll be. 


Create more inoculation points when inoculating sterile grain mediums with multiple live culture wedges of agar by shaking the grains just after inoculation.  The same principal applies when inoculating sterile grains with a single culture wedge.


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Invisibleslapphappypill
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Re: agar wedge sizing frustration. [Re: hamloaf]
    #14299236 - 04/16/11 01:32 AM (12 years, 9 months ago)

Does it make sense to cut up a bunch of small tiny pieces, say 10 the size of 1/4 a finger nail? that way when you shake it all up you have a ton of innoc points? or does that risk more contams?

I'm just thinking on the spawn bag side, if you have 6 qts worth of spawn, I'd want that to go faster without that shake at 30%, I prefer to do my only shaking right after inoculation


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I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
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North Spore Shop: Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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