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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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Spore Print Questions?
#14290514 - 04/14/11 02:43 PM (12 years, 9 months ago) |
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Should a spore print be taken on the first flush, or does it matter? I read somewhere that its best to take a print from the first flush, any reasons why this is, if its true?
If you take an outdoor print and you let the cap hang in the air above where the spores are going to be dropped, will this prevent contams from mixing in with the spore print itself?
What is the best type of mushroom to take a print from? Ive read the biggest one because they drop a lot more spores but those usually don't show up in the first flush..
If I take a print from a bigger mushroom and use that print to start the life cycle over again, would there be a better possibility that those mushrooms will be bigger, or does which mushroom I get the print from even matter? will all the mushrooms be the same anyways regardless the size or bunch of mushrooms I got the print from?
I know spore print sizes vary in sizes, but about how many 12cc syringes do you thing you can get from a decent sized print? lets say 3" diameter..
Can the print stay in distilled water for an extended period of time? such as a jar filled with distilled water, maybe with the same set up as a liquid culture jar but only with ms in it.. and possibly magnetic stir bar to help mix up the spores!
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Spore Print Questions? [Re: Deej3987]
#14290540 - 04/14/11 02:47 PM (12 years, 9 months ago) |
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Quote:
What is the best type of mushroom to take a print from?
A magic one.
-------------------- You make it all possible. Doesn't it feel good?
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Trippy_Smurf
Sketchy Mother Fucker




Registered: 02/14/11
Posts: 2,349
Loc: Smurf Villiage
Last seen: 2 years, 4 months
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Re: Spore Print Questions? [Re: Doc_T]
#14290611 - 04/14/11 02:59 PM (12 years, 9 months ago) |
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-------------------- SECURITY: READ THIS! Chef: Kids, what did I tell you about drugs? Kids: There's a time and a place for everything, and it's called college. How to be a good shroomie How to grow mushrooms A collection of good links (may be outdated) How things should look How to pass a drug test
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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Re: Spore Print Questions? [Re: Doc_T]
#14290738 - 04/14/11 03:23 PM (12 years, 9 months ago) |
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Quote:
Doc_T said:
Quote:
What is the best type of mushroom to take a print from?
A magic one. 
haha yah I'm working on that, but I meant bigger smaller? does it even matter? first/final flush? dark/light caps? a clusterfuck of mushrooms that grew together? lol
your reply definitely had me laughing 
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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cool that looks like great advice for the spore jar. So it doesn't damage the spores to scratch them into a jar?
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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Trippy_Smurf
Sketchy Mother Fucker




Registered: 02/14/11
Posts: 2,349
Loc: Smurf Villiage
Last seen: 2 years, 4 months
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Re: Spore Print Questions? [Re: Deej3987]
#14290768 - 04/14/11 03:29 PM (12 years, 9 months ago) |
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No. They like being scratched. It turns them on.
-------------------- SECURITY: READ THIS! Chef: Kids, what did I tell you about drugs? Kids: There's a time and a place for everything, and it's called college. How to be a good shroomie How to grow mushrooms A collection of good links (may be outdated) How things should look How to pass a drug test
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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ahaha I guess that would make them grow bigger!
What about all the other questions? I need to know which ones to take a print from before I start this.. I know I can take a print from any of them thats no problem, but... if theres a better one to do it off of I would like to look for that!
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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Re: Spore Print Questions? [Re: Deej3987]
#14310307 - 04/18/11 02:45 AM (12 years, 9 months ago) |
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So is it a bad idea to have a bunch of prints droped into a quart jar with the lid made like a lc lid would be made so it can be easily used to suck up spores anytime? This is what I would like to do with my prints
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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UnnamedGrower
The AMUiest



Registered: 04/17/11
Posts: 7,146
Loc: I'll be where I'm at
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Re: Spore Print Questions? [Re: Deej3987]
#14310317 - 04/18/11 02:50 AM (12 years, 9 months ago) |
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Quote:
Deej3987 said: So is it a bad idea to have a bunch of prints droped into a quart jar with the lid made like a lc lid would be made so it can be easily used to suck up spores anytime? This is what I would like to do with my prints
No this isnt any issue. Im pretty sure a vendor here sells large amounts of ms solution in a jar or bag for exactlly wat your asking.
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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So when I go to collect the print should I have the mushroom cap hang a little above where the spores will be dropping to prevent contams? or just go about it the old fashioned way on foil then scratch it off into the pre-sterilized jar?
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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UnnamedGrower
The AMUiest



Registered: 04/17/11
Posts: 7,146
Loc: I'll be where I'm at
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Re: Spore Print Questions? [Re: Deej3987]
#14310337 - 04/18/11 02:58 AM (12 years, 9 months ago) |
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Why not just print the cap right in the jar? Then add sterile water and mix the spores up. Less steps less chance of contam
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
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Re: Spore Print Questions? [Re: Deej3987]
#14310338 - 04/18/11 02:59 AM (12 years, 9 months ago) |
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print from the largest just so you get more spores. as far as capturing traits, you'll want to get into agar cloning/isolating. until then, getting 78 billion spores from one mushroom or 62 million from another will keep your genetics fresh but random.
I like to PC a water-filled syringe and an empty 1/2 pint jar (with tyvek and sealing ring) along with any grains or agar I plan on using. Once everything is cooled, transfer as cleanly as possible (SAB/GB/FH) the printing cap to the empty 1/2 pint, and let it sit for 12-24 hours. Afterward, inject your sterile water from the PC'd syringe into that 1/2 pint, give it a good swirl, tilt to pool the spore-water, and pull it back into the syringe.
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