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Spore_2_Umbrellas
Stranger

Registered: 02/20/11
Posts: 73
Last seen: 6 years, 5 months
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What tek do you use to take prints?
#14290107 - 04/14/11 01:32 PM (12 years, 9 months ago) |
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I'm asking cause I suck at taking prints...
They always seem to get contaminated or screwed up when I try to make a syringe...
How do you do it? How do you make your syringe to avoid contaminating everything?
Are you able to make many syringes with one print?
I suck so much at that..... try too hard perhaps....
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jimmyjame1
Lover



Registered: 03/13/10
Posts: 2,076
Loc:
Last seen: 5 years, 5 days
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i just take some tinfoil, and rub it down with alcohol, then i put my cap gills down on it, and place a cup upside down with a pencil holding it up to allow some ventilation.
then i fold over the excess tin foil and save for making syringes later
then to make a syringe, i just boil some water, and suck it into my syringe then hold for a few minutes then push it out. i repeat that step a few times and on the last time i hold my water in and let cool.
i will then take a clean shot glass, and a sterile butter knife or something, i scrape the spores into the glass. then i shoot the water in on top of them from the syringe. and suck it back up.
easy
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: What tek do you use to take prints? [Re: jimmyjame1]
#14290207 - 04/14/11 01:51 PM (12 years, 9 months ago) |
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you do not need to rub your foil down with alcohol...its already sterile right off the roll.
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Trippy_Smurf
Sketchy Mother Fucker




Registered: 02/14/11
Posts: 2,349
Loc: Smurf Villiage
Last seen: 2 years, 4 months
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Re: What tek do you use to take prints? [Re: jimmyjame1]
#14290210 - 04/14/11 01:52 PM (12 years, 9 months ago) |
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I actually just got done doing this...
I water sterilized a syringe (boil water, suck it into the syringe, wait a minute, empty it into the sink, repeat 9 more times), flame sterilized an exacto knife, PC'd 350ml of water in a pint jar, wiped down the jar with alcohol, and put them all in my glove box with 2 dark spore prints.
With the exacto knife, I scraped all the spores from both prints off the foil into the jar. Closed the jar tightly, and took everything out. I then swirled the jar around, which did very little to break up and distribute the spored that were floating in clumps on top of the water, so I stuck the syringe in, sucked out as many clumps of spores as I could, and then forcefully injected them back into the water. After doing that 4-5 times, the water looked pretty dirty.
I then withdrew a small amount, put a drop on agar, and injected the rest into an LC.
Hopefully when I get back on Sunday night, there will be some nice, rhizomorphic growth on the agar and a huge cloud in the LC.
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maiko maiko
I can eat 50 eggs

Registered: 02/26/10
Posts: 332
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Re: What tek do you use to take prints? [Re: jimmyjame1]
#14290271 - 04/14/11 02:04 PM (12 years, 9 months ago) |
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let the fruits mature in a closed environment, sterilize a 1/4 or 1/2 pint wide mouth jar, spray a clean room with air sanitizer, open fruiting chamber, put a tack in the cap you want to print, remove the cap with clean scissors and use the tack to transfer into the clean jar. let it sit until spores drop and then remove the cap. could also be transferred into a still air box onto pieces of foil.
or you could print in open air and transfer to agar instead of syringes
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