Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals Bridgetown Botanicals

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDeej3987
Deej3987
 User Gallery
Registered: 12/08/09
Posts: 128
Last seen: 1 year, 6 months
Re: G2G colonizing problems? [Re: deadmandave]
    #14278375 - 04/12/11 02:40 PM (12 years, 10 months ago)

Quote:

deadmandave said:
How long does it take to get through fifty lbs of rye? If it's longer than two weeks this could be a vector of contamination




To get through as in how long do all of my jars sit on the shelf before I use them? around a couple months at the most, but Ive had jars for about a year before ive used them and yes a few did get contaminated but the majority were still good. I actually used them when I started back up.The ones that did get contaminated had trich(green mold), whit mold, and some orangish mold on them.. no signs of this bacteria though. my bacterial problem doesn't show up until after ive done G2G transfer..

If your asking me how long it takes to do the whole process after the 24 hour soak then I guess...

get the water to boil probably around 1-2hrs..
drying the grains is around 2-4hrs with a fan helping since theres so much.
loading all the jars takes a while idk maybe 1.5hrs
and PCing only takes about 4hrs since I have to wait for the pressure to de-pressurize and cool off a bit before I put it all away.. and yes I pc them at 15psi for 90mins

I can do it all in around 10 hrs


--------------------
My Trade List

Printing


All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.

Extras: Filter Print Post Top
OfflineDeej3987
Deej3987
 User Gallery
Registered: 12/08/09
Posts: 128
Last seen: 1 year, 6 months
Re: G2G colonizing problems? [Re: deadmandave]
    #14278497 - 04/12/11 03:00 PM (12 years, 10 months ago)

If I flame sterilize the needle how do I prevent it from rusting later? also if I flame sterilize in my GB I can see that being a problem.. Ive had a lot of success with just wiping the needle down with the highest % alcohol and cotton balls. Its only recently Ive been having the bacterial problem..

Idk maybe this problem has always been there its just now starting to show up because its the only contam problem I run into anymore besides like 1 green mold jar out of every 500 jars..

Black mold was a problem last year, I put the jars in the garage when the weather was hot, like around 100F in the garage... My reason for doing this was because myc started colonizing a lot faster out there than inside in the air conditioning.. I know that temp is close to thermal death for the myc.. 81F-83F is ideal temp I know..

But like I said right now the only problem I run into anymore is the bacteria.. I think I can get around flame sterilization someone correct me if I'm wrong though?


--------------------
My Trade List

Printing


All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.

Extras: Filter Print Post Top
Offlinemabachko
Just Strange
 User Gallery


Registered: 02/11/11
Posts: 702
Last seen: 8 years, 7 months
Re: G2G colonizing problems? [Re: Deej3987]
    #14278665 - 04/12/11 03:27 PM (12 years, 10 months ago)

If your not doing something the right way & are having problems, wouldn't that be the first step to take for removing the problem??

Extras: Filter Print Post Top
Offlinedeadmandave
Slime
Male User Gallery

Registered: 02/16/10
Posts: 3,372
Loc: Flag
Last seen: 3 hours, 35 minutes
Trusted Cultivator
Re: G2G colonizing problems? [Re: mabachko]
    #14278694 - 04/12/11 03:35 PM (12 years, 10 months ago)

You need to flame sterilize. Do a few searches, look at trusted techniques. IF you are not flame sterilizing you are only sanitizing your needle. You greatly increase your rate of contamination by sanitizing with alcohol rather than flamer sterilizing. .... This is a fact. If your needle rusts then get a new one. :awesome:

Also when you prep that much rye berries at a time to just sit around, you are creating another way contaminants can take over your jars. Sterilizing does not kill everything and given enough time whatever is left inside the jar will take over. Pressure cooking and sterilizing jars only leaves a window of opportunity for you to add mycelium and let it colonize, it does not create a permanent clean slate for you to grow mushrooms.

Extras: Filter Print Post Top
OfflineDeej3987
Deej3987
 User Gallery
Registered: 12/08/09
Posts: 128
Last seen: 1 year, 6 months
Re: G2G colonizing problems? [Re: deadmandave]
    #14278848 - 04/12/11 04:14 PM (12 years, 10 months ago)

yes but who's to say wiping the syringe down with cotton balls and alcohol isn't the right way? there are different methods for sterilization such as GB or hood flow.. nether of these methods are wrong they are just different methods to go about keeping contams from getting on the substrate.

If I flame sterilize the needle it will rust.. then I have to buy more.. I am going to pc them and continue re-using them the same way..  My bacterial problem could also be in the LC like said earlier.

I do not believe flame sterilization is my problem.

I would like to see more answers than just "flame sterilizing is the law".. I want actual reasons as to why wiping with alcohol is a no no.

bacteria does not die off from alcohol?

Quote:

deadmandave said:
You need to flame sterilize. Do a few searches, look at trusted techniques. IF you are not flame sterilizing you are only sanitizing your needle. You greatly increase your rate of contamination by sanitizing with alcohol rather than flamer sterilizing. .... This is a fact. If your needle rusts then get a new one. :awesome:

Also when you prep that much rye berries at a time to just sit around, you are creating another way contaminants can take over your jars. Sterilizing does not kill everything and given enough time whatever is left inside the jar will take over. Pressure cooking and sterilizing jars only leaves a window of opportunity for you to add mycelium and let it colonize, it does not create a permanent clean slate for you to grow mushrooms.




alcohol increases the rate of contams on the needle? so it would be better to not even wipe it with alcohol?

how do I stop the needle from rusting? I used a lighter when I did it before.. does an actual scientific burner with alcohol in it prevent the rust? srry I don't remember what they're called.. I'm trying to be efficient, I don't want to keep buying syringes..

I know what you are saying about the small window of contams, but also like I said I have no contams until I G2G transfer later on.. I know contams can get in there ive seen it happen, I keep the lids covered with the aluminum foil or cover with plastic until I use them to help prevent this.. I just don't see contams until after ive used them..

also people sell sterilized substrate online which stays sterile until they're used.. I'm thinking my problem could be the initial needle being a little dirty or a problem with my g2g sterile techniques..

Ill look up techniques for flame sterilization, maybe you could send me some links? I understand why it would be the best method to prevent contams, but if I have to keep buying new needles why not just pc them after every use and wipe them with alcohol before every use.. that would be more efficient if it works only because I'm re-using the syringes instead of constantly buying new ones.


--------------------
My Trade List

Printing


All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.

Extras: Filter Print Post Top
OfflinePrimal Call
Earth Mage
I'm a teapot


Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 4 months
Re: G2G colonizing problems? [Re: Deej3987]
    #14278937 - 04/12/11 04:39 PM (12 years, 10 months ago)

Quote:

Deej3987 said:

I'm going to pc after a few uses for now on and maybe that will stop the bacterial growth..




this is part of the problem IMO. the only time I can imagine using less than a full syringe is to test a new LC or spore syringe you made yourself if you aren't using agar. the next time you use it, it should be emptied and then PC'd again just prior to its next use.

also, flame sterilizing is better than alcohol. key word: sterilizing. it probably is not 100% sterile, but it is assuredly better than sanitizing with alcohol. key word sanitizing.

lastly, preparing that much rye and letting it sit around is a bad move. you should prep as much as you will use the next inoculation "round" and no more. by letting them sit out, you are giving any contaminates a head-start in the race before you even introduce anything mushroom-related. not to mention, if you are using something other than dikaryotic mycelia (ie: spores) you have to wait for germination before they even try eating your grain, and this gives contams an even greater lead.


--------------------
New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


:peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace:

Extras: Filter Print Post Top
InvisiblePhoenix Crash
Lesbian Seagull
Male


Registered: 10/20/09
Posts: 482
Loc: From Paris With Love
Re: G2G colonizing problems? [Re: Primal Call]
    #14279087 - 04/12/11 05:13 PM (12 years, 10 months ago)

Ryath has told you NO lies, my friend.

Your technique: Wipe needle tip with alcohol to sanitize.

Problem with that technique: Wiping with alcohol only 'sanitizes' the outside of the needle tip. The reason for flaming the tip is it 'sterilizes' the outside and inside of the tip with the intense heat.

Your technique: PC an amount of rye jars that sit around until you have the time to noc up 100 jars.

Problem with that: Like Ryath said, PCing the rye only leaves you a window to inject spores or LC. After sitting for a few days, bacteria, mold, and cobweb usually set in, no matter what jar lids you use.

Your technique: Not sterilizing your syringes properly because you say the PC 'melts' them.

Problem with that one: Don't PC them at all. Fill a pot with water and bring it to a rolling boil for 30 minutes. Take the syringe (with or without tip) and fill it with boiling water. Let this water stay in the syringe for ~1 minute, then expel the water into the sink, not back into the pot. Repeat this 4 times, leaving the boiling water in the syringe on the last fill-up. As an extra step, you can fill and empty the syringe 4 times as mentioned above, except empty the syringe on the last fill and wrap it in aluminum foil. Toss the foil-wrapped syringe into the boiling water, slap a lid on the pot, and let it go for 25-30 minutes. No need for a PC, and I don't know how to melt a syringe using this method.

Not trying to come off as a dick or anything bro, just thought I would backup some of the correct info others have already given you on here and offer you some of my advice. It has yet to fail me. If you really want to see the end of this contamination problem you're having, you're gonna have to let go of what you 'think' you 'know' works, and open your mind to the advice of others who have probably already been there and got rid of that problem. Good luck.


--------------------
Why is pot against the law? It wouldn't be because anyone can grow it, and therefore you can't make a profit off it, would it? - Bill Hicks



Phoenix Crash LC Tek
DIY Magnetic Stir Bars Tek

"Not all drugs are good, all right? Some of them… are fuckin' great. Just gotta know your way around them, is all."

Extras: Filter Print Post Top
OfflinePrimal Call
Earth Mage
I'm a teapot


Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 4 months
Re: G2G colonizing problems? [Re: Phoenix Crash]
    #14279297 - 04/12/11 05:52 PM (12 years, 10 months ago)

on the whole melting syringe thing, I thought I should update... I had a recent PC round unintentionally hold 17PSI for about an hour, and it melted the needle cap. the actual syringe was still fine :shrug:

and thx Phoenix :smile:

:peace:


--------------------
New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


:peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace:

Extras: Filter Print Post Top
OfflineDeej3987
Deej3987
 User Gallery
Registered: 12/08/09
Posts: 128
Last seen: 1 year, 6 months
Re: G2G colonizing problems? [Re: Primal Call]
    #14282620 - 04/13/11 08:05 AM (12 years, 10 months ago)

Quote:

Ryath said:
Quote:

Deej3987 said:

I'm going to pc after a few uses for now on and maybe that will stop the bacterial growth..




this is part of the problem IMO. the only time I can imagine using less than a full syringe is to test a new LC or spore syringe you made yourself if you aren't using agar. the next time you use it, it should be emptied and then PC'd again just prior to its next use.

also, flame sterilizing is better than alcohol. key word: sterilizing. it probably is not 100% sterile, but it is assuredly better than sanitizing with alcohol. key word sanitizing.

lastly, preparing that much rye and letting it sit around is a bad move. you should prep as much as you will use the next inoculation "round" and no more. by letting them sit out, you are giving any contaminates a head-start in the race before you even introduce anything mushroom-related. not to mention, if you are using something other than dikaryotic mycelia (ie: spores) you have to wait for germination before they even try eating your grain, and this gives contams an even greater lead.




hmm alright Ill have to empty my syringes after every use and pc them for now on. and about the melting the syringe, its hard to explain but the part the needle twist onto the part with all the water was the part that became deformed a little as if it melted obviously.. idk the needle wasn't straight like usual, or the needle was straight it just wasn't at a 180 degree angle. I was able to still use it but if I kept pc it every time eventually it was gonna be to F*cked up... I never wrapped it in aluminum foil I will try this and do the boiling water thing thank you guys for the advice.


do your guys needles rust? big question because I need to know if I need to buy an alcohol lamp to avoid this problem..

also about the grains getting contamed for sitting there to long... I understand what you are saying about the window of contams.. I understand that.. My question is (if that is the case) why doesn't the bacteria show up until after Ive inoc or made a g2g transfer?

The grains look completely fine until I have messed with them.. If trich or white mold gets into the jars before I mess with them, I will definitely be able to tell.. My only contamination issue right now is the bacterial growth and I'm pretty sure its from the initial syringes.. Or maybe my gb is to big..

Quote:

Ryath said:

not to mention, if you are using something other than dikaryotic mycelia (ie: spores) you have to wait for germination before they even try eating your grain, and this gives contams an even greater lead.





does this mean for example.. the bacteria is there I just cant see it until after the myc has germinated and started to take over the substrate?


--------------------
My Trade List

Printing


All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.

Extras: Filter Print Post Top
OfflinePrimal Call
Earth Mage
I'm a teapot


Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 4 months
Re: G2G colonizing problems? [Re: Deej3987]
    #14284863 - 04/13/11 04:17 PM (12 years, 10 months ago)

Quote:

Deej3987 said:
Quote:

Ryath said:

not to mention, if you are using something other than dikaryotic mycelia (ie: spores) you have to wait for germination before they even try eating your grain, and this gives contams an even greater lead.




does this mean for example.. the bacteria is there I just cant see it until after the myc has germinated and started to take over the substrate?




it could be, it could not be. the only way you can tell is if you leave an un-inoculated jar out for many weeks and see if the contaminates show up. if this happens, you are not preparing your jars correctly in the PC or the lid setup. My point with that post is that the longer you leave your grain without dikaryotic mycelia covering it, the more you increase the chances of contaminates taking over those nutrients.

if you are only seeing contaminates after you inoculate or G2G, your work area and/or sterile technique need improving in the cleanliness department. you can't half-ass it. Well, you can, but it will greatly increase your contamination rate. I learned this, as a lot of us do, the hard way. Laziness does not pay off with this hobby.


--------------------
New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


:peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace:

Extras: Filter Print Post Top
OfflineDeej3987
Deej3987
 User Gallery
Registered: 12/08/09
Posts: 128
Last seen: 1 year, 6 months
Re: G2G colonizing problems? [Re: Primal Call]
    #14286837 - 04/13/11 09:31 PM (12 years, 10 months ago)

ok cool thanks for the advice. so I am pc everything correctly and have had close to no problems with contams on the jars as they are sitting there (shelf life). Time to crack down on these syringes for starters. I will not leave anything in the syringe when I am done enoc for now on..

As far as flame sterilizing... I know its the best method, but I will try pc the syringes and continue what I'm doing before I move on to that method. I know I'm bull headed but like you said, I'm gonna have to learn this the hard way :evil:

Ill post more on my results later.


--------------------
My Trade List

Printing


All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.

Extras: Filter Print Post Top
OfflinePrimal Call
Earth Mage
I'm a teapot


Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 4 months
Re: G2G colonizing problems? [Re: Deej3987]
    #14287117 - 04/13/11 10:18 PM (12 years, 10 months ago)

:lol: why not just do it, man? all you gotta do is add a lighter or a candle to your worklist...


--------------------
New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


:peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace:

Extras: Filter Print Post Top
Offlinedeadmandave
Slime
Male User Gallery

Registered: 02/16/10
Posts: 3,372
Loc: Flag
Last seen: 3 hours, 35 minutes
Trusted Cultivator
Re: G2G colonizing problems? [Re: Primal Call]
    #14288151 - 04/14/11 01:51 AM (12 years, 10 months ago)

if you're using alcohol to sanitize you may as well just build an alcohol lamp.

use a 1/2 pint jar, poke one hole in the lid. Fill it <1 inch with 90% isopropol alcohol. Soak a cotton string/rope in alcohol and then pull it through the hole in the jar lid. Here is an awesome picture from mycocurious:

I have used needles over and over and over and have never had one rust on me, but i will tell you the tips get dull after only a few uses regardless :shrug:

Extras: Filter Print Post Top
OfflinePrimal Call
Earth Mage
I'm a teapot


Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 4 months
Re: G2G colonizing problems? [Re: deadmandave]
    #14288174 - 04/14/11 01:59 AM (12 years, 10 months ago)

awesome! gonna make one of those tomorrow! thx DMD! :laugh::super:

would 70% be good enough?


--------------------
New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


:peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace::peace:

Extras: Filter Print Post Top
OfflineDeej3987
Deej3987
 User Gallery
Registered: 12/08/09
Posts: 128
Last seen: 1 year, 6 months
Re: G2G colonizing problems? [Re: deadmandave]
    #14289503 - 04/14/11 11:25 AM (12 years, 10 months ago)

I tried making one of these with just a cotton ball and every time I lighted it the alcohol made its way up to the top of the lid and the flame engolfed the hole top part.. I guess I need that cotton string thing, where would I find it? what size hole?


--------------------
My Trade List

Printing


All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.

Extras: Filter Print Post Top
Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: G2G colonizing problems? [Re: Deej3987]
    #14289592 - 04/14/11 11:43 AM (12 years, 10 months ago)

cotton ball can work. Problem was that air differential from the flame was pulling too much liquid thru, which pools on the surface and catches fire.

Solution is to add a small pinhole off to the side which alleviates the pressure and stabilizes the flame.

Now u kno.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

Extras: Filter Print Post Top
OfflineDeej3987
Deej3987
 User Gallery
Registered: 12/08/09
Posts: 128
Last seen: 1 year, 6 months
Re: G2G colonizing problems? [Re: anonjon]
    #14289618 - 04/14/11 11:47 AM (12 years, 10 months ago)

Quote:

anonjon said:
cotton ball can work. Problem was that air differential from the flame was pulling too much liquid thru, which pools on the surface and catches fire.

Solution is to add a small pinhole off to the side which alleviates the pressure and stabilizes the flame.

Now u kno.




thank you so much!


--------------------
My Trade List

Printing


All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals Bridgetown Botanicals


Similar ThreadsPosterViewsRepliesLast post
* Slow Colonization Problem *DELETED* oneducktwoducks 945 6 02/13/03 05:42 PM
by Hexum311
* colonization problems!! HELP! Anonymous 564 2 04/22/02 09:21 PM
by ParticleMan
* Bottom of Jars not colonizing SpaceCruiser 3,421 2 04/25/02 07:39 AM
by frank_grimes
* G2G Transfer? lvleph 4,469 9 11/20/02 12:28 PM
by scifi5001
* G2G transfers? lvleph 4,153 15 11/03/13 06:15 AM
by SpitballJedi
* problems with grain quarts RedHead 3,585 5 04/02/02 07:37 PM
by RedHead
* Innoculating from colonized jar Bandit 1,530 7 01/12/03 11:23 AM
by sirreal
* Help with stalled cake colonization psyphon 2,177 6 12/05/01 03:50 PM
by psyphon

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,642 topic views. 24 members, 162 guests and 46 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.007 seconds on 13 queries.