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thesoundd
Candy Coater


Registered: 04/14/10
Posts: 178
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P. mexicana A fruiting on agar...now what?
#14282997 - 04/13/11 10:05 AM (12 years, 9 months ago) |
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I haven't tried to fruit my P. mexicana before because it seems a bit pickier than cubes. However, I just discovered that one of my agar plates is sending up little fruiting bodies!
Is there any logic in using the mushrooms to grow out a strain that I can then use for fruiting? RR suggested that I isolate the sclerotia that form on the plates as a way to develop a more prolific strain as far as sclerotia production goes...but does the same logic apply to fruiting bodies? Thanks!
Also, FYI, the plate was inoculated from an agar wedge and its been incubating at ~70° for ~1.5 months and this is my medium recipe:
Race for the Prize agar:
3 g Tryptone 3 g Soytone 2 g Yeast Extract 20 g Dextrose 5 g Maltose 20 g Agar Adjust pH to 6 Up to 1 L with dH2O
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esquaredx



Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
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Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
#14283023 - 04/13/11 10:11 AM (12 years, 9 months ago) |
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I wunna see a piccc
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M11
White Thumb

Registered: 06/14/09
Posts: 1,840
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Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
#14283026 - 04/13/11 10:11 AM (12 years, 9 months ago) |
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P. mexicanae fruiting bodies before sclerotia formation? That is very odd. You sure it isn't just the beginning of a stone?
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: P. mexicana A fruiting on agar...now what? [Re: M11]
#14283039 - 04/13/11 10:15 AM (12 years, 9 months ago) |
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Pics? Also, yes- clone and grow.
-------------------- You make it all possible. Doesn't it feel good?
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thesoundd
Candy Coater


Registered: 04/14/10
Posts: 178
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Re: P. mexicana A fruiting on agar...now what? [Re: M11]
#14283049 - 04/13/11 10:18 AM (12 years, 9 months ago) |
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Do a search for mexicana fruiting bodies on agar, you'll see images posted by RR. Of course, his plates look much more prolific than mine 
There are sclerotia forming on the plate, but the fruiting bodies aren't growing out of the stones, they're coming out of mycelia. They're definitely mushrooms (granted, a little queer looking in their cramped quarters).
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esquaredx



Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
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Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
#14283061 - 04/13/11 10:20 AM (12 years, 9 months ago) |
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You want us to look at other peoples agar plate?  Whats wrong with yours...?
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cyanara
jedi in training



Registered: 12/22/09
Posts: 1,205
Loc: your grow closet
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Re: P. mexicana A fruiting on agar...now what? [Re: M11]
#14283066 - 04/13/11 10:22 AM (12 years, 9 months ago) |
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that a really long time for agar to sit at room temp. too long actualy and your agar receipy is way too rich, you have too much in there..I would reduce your detros level to 10 grams per liter and either remove your soytone or tryptone and maybe even reduce your yeast to 1 gram per liter. too much makes your mycelium lazy and will cause mutation in your mycelium and lead to poor fruiting strains. As far as the fruit forming on the agar, after preparing a new batch, I would take the fastest growing specimen and when cooling the scalpel in the transfer dish, make it nice and wide to be able to receive the new fruit body. transfer deep into the agar, not just on the surface. Having the agar touching all sides will allow for rapid mycelium development from the transfered fruit.
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thesoundd
Candy Coater


Registered: 04/14/10
Posts: 178
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Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
#14283077 - 04/13/11 10:24 AM (12 years, 9 months ago) |
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Or here, I'll do the work for you.
Quote:
RogerRabbit said:
 Mexicana Jalisco fruiting from sclerotia on agar
Granted, this is Jalisco and not mexicana A, but I don't imagine it makes too much of a difference for this scenario.
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esquaredx



Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
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Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
#14283087 - 04/13/11 10:26 AM (12 years, 9 months ago) |
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Quote:
thesoundd said: Or here, I'll do the work for you.
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thesoundd
Candy Coater


Registered: 04/14/10
Posts: 178
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Re: P. mexicana A fruiting on agar...now what? [Re: cyanara]
#14283118 - 04/13/11 10:31 AM (12 years, 9 months ago) |
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Quote:
cyanara said: that a really long time for agar to sit at room temp. too long actualy and your agar receipy is way too rich, you have too much in there..I would reduce your detros level to 10 grams per liter and either remove your soytone or tryptone and maybe even reduce your yeast to 1 gram per liter. too much makes your mycelium lazy and will cause mutation in your mycelium and lead to poor fruiting strains. As far as the fruit forming on the agar, after preparing a new batch, I would take the fastest growing specimen and when cooling the scalpel in the transfer dish, make it nice and wide to be able to receive the new fruit body. transfer deep into the agar, not just on the surface. Having the agar touching all sides will allow for rapid mycelium development from the transfered fruit.
That's very useful information. I always wondered why I never see rhizo-type growth on my plates. I can certainly tone down the ingredients...I guess I was inadvertently killing them with love, or something along those lines. Also, what is the harm of the plate sitting out for a long time? I wrap my plates in parafilm and I throw them out on the rare occasion that contamination pops up. Does this also lead to unhelpful mutations?
Also, I'm not completely sure of what you mean in your transfer directions. Are you saying that I should cut a hole out of a fresh plate and put the fruiting body in, or that I should sink the fruiting body into the agar while it is still a liquid?
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