Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Mix   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblethesoundd
Candy Coater
Male User Gallery

Registered: 04/14/10
Posts: 178
P. mexicana A fruiting on agar...now what?
    #14282997 - 04/13/11 10:05 AM (12 years, 9 months ago)

I haven't tried to fruit my P. mexicana before because it seems a bit pickier than cubes. However, I just discovered that one of my agar plates is sending up little fruiting bodies!

Is there any logic in using the mushrooms to grow out a strain that I can then use for fruiting? RR suggested that I isolate the sclerotia that form on the plates as a way to develop a more prolific strain as far as sclerotia production goes...but does the same logic apply to fruiting bodies? Thanks!

Also, FYI, the plate was inoculated from an agar wedge and its been incubating at ~70° for ~1.5 months and this is my medium recipe:

Race for the Prize agar:

3 g Tryptone
3 g Soytone
2 g Yeast Extract
20 g Dextrose
5 g Maltose
20 g Agar
Adjust pH to 6
Up to 1 L with dH2O


Extras: Filter Print Post Top
Offlineesquaredx
 User Gallery


Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
    #14283023 - 04/13/11 10:11 AM (12 years, 9 months ago)

I wunna see a piccc


--------------------


Extras: Filter Print Post Top
InvisibleM11
White Thumb

Registered: 06/14/09
Posts: 1,840
Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
    #14283026 - 04/13/11 10:11 AM (12 years, 9 months ago)

P. mexicanae fruiting bodies before sclerotia formation?  That is very odd.  You sure it isn't just the beginning of a stone?


--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
-RR

Those mushrooms are fine.  Your friend is a pussy.
-RR

Outdoor Patch


Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: P. mexicana A fruiting on agar...now what? [Re: M11]
    #14283039 - 04/13/11 10:15 AM (12 years, 9 months ago)

Pics? Also, yes- clone and grow.


--------------------
You make it all possible. Doesn't it feel good?


Extras: Filter Print Post Top
Invisiblethesoundd
Candy Coater
Male User Gallery

Registered: 04/14/10
Posts: 178
Re: P. mexicana A fruiting on agar...now what? [Re: M11]
    #14283049 - 04/13/11 10:18 AM (12 years, 9 months ago)

Do a search for mexicana fruiting bodies on agar, you'll see images posted by RR. Of course, his plates look much more prolific than mine :crazy:

There are sclerotia forming on the plate, but the fruiting bodies aren't growing out of the stones, they're coming out of mycelia. They're definitely mushrooms (granted, a little queer looking in their cramped quarters).


--------------------


Extras: Filter Print Post Top
Offlineesquaredx
 User Gallery


Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
    #14283061 - 04/13/11 10:20 AM (12 years, 9 months ago)

You want us to look at other peoples agar plate? :confused:
Whats wrong with yours...?


--------------------


Extras: Filter Print Post Top
Invisiblecyanara
jedi in training
Male


Registered: 12/22/09
Posts: 1,205
Loc: your grow closet
Re: P. mexicana A fruiting on agar...now what? [Re: M11]
    #14283066 - 04/13/11 10:22 AM (12 years, 9 months ago)

that a really long time for agar to sit at room temp. too long actualy and your agar receipy is way too rich, you have too much in there..I would reduce your detros level to 10 grams per liter and either remove your soytone or tryptone and maybe even reduce your yeast to 1 gram per liter. too much makes your mycelium lazy and will cause mutation in your mycelium and lead to poor fruiting strains.
As far as the fruit forming on the agar, after preparing a new batch, I would take the fastest growing specimen and when cooling the scalpel in the transfer dish, make it nice and wide to be able to receive the new fruit body. transfer deep into the agar, not just on the surface. Having the agar touching all sides will allow for rapid mycelium development from the transfered fruit.


Extras: Filter Print Post Top
Invisiblethesoundd
Candy Coater
Male User Gallery

Registered: 04/14/10
Posts: 178
Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
    #14283077 - 04/13/11 10:24 AM (12 years, 9 months ago)

Or here, I'll do the work for you.

Quote:

RogerRabbit said:

Mexicana Jalisco fruiting from sclerotia on agar




Granted, this is Jalisco and not mexicana A, but I don't imagine it makes too much of a difference for this scenario.


--------------------


Extras: Filter Print Post Top
Offlineesquaredx
 User Gallery


Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
Re: P. mexicana A fruiting on agar...now what? [Re: thesoundd]
    #14283087 - 04/13/11 10:26 AM (12 years, 9 months ago)

Quote:

thesoundd said:
Or here, I'll do the work for you.




:rofldrunk:


--------------------


Extras: Filter Print Post Top
Invisiblethesoundd
Candy Coater
Male User Gallery

Registered: 04/14/10
Posts: 178
Re: P. mexicana A fruiting on agar...now what? [Re: cyanara]
    #14283118 - 04/13/11 10:31 AM (12 years, 9 months ago)

Quote:

cyanara said:
that a really long time for agar to sit at room temp. too long actualy and your agar receipy is way too rich, you have too much in there..I would reduce your detros level to 10 grams per liter and either remove your soytone or tryptone and maybe even reduce your yeast to 1 gram per liter. too much makes your mycelium lazy and will cause mutation in your mycelium and lead to poor fruiting strains.
As far as the fruit forming on the agar, after preparing a new batch, I would take the fastest growing specimen and when cooling the scalpel in the transfer dish, make it nice and wide to be able to receive the new fruit body. transfer deep into the agar, not just on the surface. Having the agar touching all sides will allow for rapid mycelium development from the transfered fruit.




That's very useful information. I always wondered why I never see rhizo-type growth on my plates. I can certainly tone down the ingredients...I guess I was inadvertently killing them with love, or something along those lines. Also, what is the harm of the plate sitting out for a long time? I wrap my plates in parafilm and I throw them out on the rare occasion that contamination pops up. Does this also lead to unhelpful mutations?

Also, I'm not completely sure of what you mean in your transfer directions. Are you saying that I should cut a hole out of a fresh plate and put the fruiting body in, or that I should sink the fruiting body into the agar while it is still a liquid?


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Mix   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Re: i this a picture of psilocybe mexicana fruiting in a petri dish? mycofile 4,739 10 02/05/00 11:45 AM
by Mycelium5150
* What's the deal with Sclerotia???
( 1 2 all )
too_many_weirdos 9,278 27 10/19/05 02:33 AM
by blackout
* Does Psilocybe mexicana Jalisco Strain produce sclerotia? theshiftingwalls 4,231 2 12/06/03 12:33 PM
by automan
* Answering questions on Ps. Mexicana
( 1 2 3 all )
Cyber 5,999 45 10/31/04 01:00 PM
by Cyber
* mexicana truffle grow nick_hopton 3,731 16 10/20/04 07:47 AM
by blacklabrat
* sclerotia formation casing/invitro OJK 2,853 10 04/03/04 11:17 AM
by OJK
* Im going to grow sclerotia invitro. ideas? too_many_weirdos 2,320 6 02/14/02 04:24 PM
by Anonymous
* Re: P. Mexicana
( 1 2 all )
Anonymous 4,671 31 01/31/00 10:25 AM
by Condi1

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,484 topic views. 26 members, 143 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.008 seconds on 14 queries.