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OfflineNotMyName
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Concerns about first monotub
    #14274919 - 04/11/11 09:13 PM (12 years, 10 months ago)

Hey guys. I'm doing my first monotub, and I have a couple of concerns. I used Doc's wbs tek and Damion5050's coir tek. After letting the casing colonize for ~13 days, I put it into fruiting (April 5th). I have a small fan running in the closet that the tub is in and a lamp pointed at it that I do my best to keep on a 12/12 schedule.
It is now April 11th and I see no signs of pinning. How long is it supposed to take before I see them? Excuse my horribly uneven casing layer, I was in a bit of a rush and didn't consider that it needed to be level.



Also, the myc is turning an interesting yellowish-brown, especially in this one corner. Should I be worried?



Any other observations/suggestions are very welcome.


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OfflineBuckeye Oysters
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Re: Concerns about first monotub [Re: NotMyName]
    #14274946 - 04/11/11 09:19 PM (12 years, 10 months ago)

Your mycellium is a bit dry at the corners, your are not misting enough.  You should be misting 3x a day, just enough so that 10 minutes later the mist is mostly evaporated into the tub air.  But you need to first give it a good heavy misting to rehydrate it.

Also fan out the air in the tub 3x+ a day

Should see pins in about 3 days id say.


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Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.

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OfflineNotMyName
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Re: Concerns about first monotub [Re: Buckeye Oysters]
    #14274969 - 04/11/11 09:22 PM (12 years, 10 months ago)

Hmm, I have been misting it as I see that the walls of the tub are dry, but I will definitely try kicking it up to 3x a day. What's the best way to actually mist it without hurting anything? Spray it against the walls?


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OfflineBobthehobo9
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Re: Concerns about first monotub *DELETED* [Re: NotMyName]
    #14275247 - 04/11/11 10:03 PM (12 years, 10 months ago)

Post deleted by Bobthehobo9

Reason for deletion: .



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OfflineNotMyName
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Re: Concerns about first monotub [Re: Bobthehobo9]
    #14275293 - 04/11/11 10:11 PM (12 years, 10 months ago)

I have no idea what my humidity is at and I don't really have a way to tell. I'm on a limited budget for now, but a hygrometer is definitely on my list. 10-12 days? Maybe I'm getting a little jumpy and should just give it more time then.


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OfflineEn passant
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Re: Concerns about first monotub [Re: NotMyName]
    #14275329 - 04/11/11 10:16 PM (12 years, 10 months ago)

Unless those glints of light in the pics are vermiculite bouncing a flash, it looks properly hydrated to me.  If it glistens, it's fine.

A 13 day casing colonization seems a bit long to me.  From the bottom pic, it looks like the fungal mass is still in its vegetative stage.  What likely happened is that when it reached the top of the casing layer and didn't get fruiting signals, it "thought" it was in a nutrient poor environment and continued seeking nutrients.  Introducing fruiting conditions to such a large mass of fungus that is biochemically primed for nutrient acquisition is going to have a slow, unsatisfying effect.

I recommend scratching those snowy caps down and adding a thin casing layer on top...

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OfflineNotMyName
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Re: Concerns about first monotub [Re: En passant]
    #14275508 - 04/11/11 10:44 PM (12 years, 10 months ago)

The reason I let it colonize so long is that I first thought that the top had to be covered fully in myc. When I realized that this was not the case, I put it into fruiting as soon as I could. What I think you're seeing 'glisten' is a bunch of new little dots of myc that have poked through.

Scratching the top? And if I do another thin layer, should I put it only on the already exposed myc, or over the whole thing?


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OfflineMad River
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Re: Concerns about first monotub [Re: NotMyName]
    #14275583 - 04/11/11 10:56 PM (12 years, 10 months ago)

Good luck. If you ask me, casing, fanning and misting a monotub are just asking for trouble. If you have a properly hydrated sub to begin with, none of that is necessary.

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Offlineyessir
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Re: Concerns about first monotub [Re: Mad River]
    #14275701 - 04/11/11 11:28 PM (12 years, 10 months ago)

Isn't the monotub suppose to eliminate misting and fanning? Quick question, do you induce pinning before closing it up? I'm thinking yes.

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OfflineEn passant
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Re: Concerns about first monotub [Re: yessir]
    #14275756 - 04/11/11 11:45 PM (12 years, 10 months ago)

Scratching the surface tears that fungal mass up.  Each cell in that mass is communicating "keep growing" to each of its neighbors and creating a sort of vegetative "feedback loop".  If you scratch it down with a fork, that reduces that feedback loop and allows a new one to begin, a reproductive feedback loop.


As to whether or not you should recase the whole thing, that's up to you.  From what I see, you have overlay all over the place, so it would be worth scratching and recasing the whole thing. For future reference, those halos of spreading mycelia around the large masses are an indication that the masses are still in the vegetative stage.  You want to introduce fruiting conditions when what I saw as "glisten" appears.  Given the extent of overlay, I think a complete scratch and recase is in order.

However, if you look closely and see hyphal knots in some areas, those areas are already in the reproductive phase and you have to make a choice.  If you scratch and recase, those areas will likely never fruit; if you don't scratch and recase, they will likely be the only areas that fruit.  Hopefully (and most likely) there's absolutely no reproductive activity, and you can recase the whole thing.

And remember, if you do recase, use a very thin layer and keep it in fruiting conditions.  You won't see pinning on the surface, but it'll be occurring at the level of your current casing.

Live, learn, and grow.

Edited by En passant (04/12/11 12:13 AM)

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OfflineNotMyName
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Re: Concerns about first monotub [Re: En passant]
    #14279641 - 04/12/11 07:01 PM (12 years, 10 months ago)

First of all, thanks for all the great responses so far. I don't have any materials on hand to make a proper casing layer, so can I use a thin layer of verm? Sterilize it first? Should I only case the areas that are exposed or the whole thing? I've been waiting on this thing for what seems like forever and I don't want to screw it up anymore than it is.


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Invisibleanonjon
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Re: Concerns about first monotub [Re: NotMyName]
    #14279866 - 04/12/11 07:41 PM (12 years, 10 months ago)

Can u take some macros of the beige / brownish spots?


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Offlinek00laid
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Re: Concerns about first monotub [Re: En passant]
    #14279889 - 04/12/11 07:44 PM (12 years, 10 months ago)

Quote:

En passant said:
If you scratch it down with a fork, that reduces that feedback loop and allows a new one to begin, a reproductive feedback loop.





the fungal mass being torn and scratched IS NOT a pinning trigger.


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Offlinek00laid
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Re: Concerns about first monotub [Re: k00laid]
    #14279897 - 04/12/11 07:45 PM (12 years, 10 months ago)

and there is no overlay in ur tub.


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OfflineNotMyName
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Re: Concerns about first monotub [Re: k00laid]
    #14280520 - 04/12/11 09:13 PM (12 years, 10 months ago)


Any other pictures?


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OfflinePrimal Call
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Re: Concerns about first monotub [Re: NotMyName]
    #14280768 - 04/12/11 09:47 PM (12 years, 10 months ago)

Quote:

NotMyName said:
First of all, thanks for all the great responses so far. I don't have any materials on hand to make a proper casing layer, so can I use a thin layer of verm? Sterilize it first? Should I only case the areas that are exposed or the whole thing? I've been waiting on this thing for what seems like forever and I don't want to screw it up anymore than it is.




don't sterilize a casing

in the OP, you say it had colonized for 13 days and been in fruiting for 6 days, but the pics there aren't even fully colonized yet. were they taken earlier?

the fan on 24/7 in a contained environment (closet) could be drying out your sub. note: substrate pulling away from the sides of the FC pre-pinning, little condensation on your tub walls, and no "misty-dew" on your surface.

regardless, it's been in fruiting for a week now, and sometimes it takes more than 10 days to pin. it's not "normal," but it can happen. personally, this is why I like to let the sub stay in colonization for as long as necessary until they tell me when to move to fruiting conditions ie: knots/pins showing. recently, this was in 13 and 11 days (separate projects) after spawning WBS to HVCCG.

hope this helps and that we troubleshoot this for you!
:mushroom2: vibes your way! :trippinballs:


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OfflineMad River
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Re: Concerns about first monotub [Re: Primal Call]
    #14280839 - 04/12/11 09:58 PM (12 years, 10 months ago)

:whathesaid:

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OfflineNotMyName
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Re: Concerns about first monotub [Re: Primal Call]
    #14281329 - 04/12/11 11:10 PM (12 years, 10 months ago)

I suppose I meant pasteurize then, sorry. Ok, that makes me feel a little better. I actually turned the fan off a couple days ago for that very reason. I've been trying to keep it misted to the point of the walls always having condensation after letting it dry out that first time. I'll give it another couple of days then and see what happens. If nothing else, I've got another set of jars just almost ready to go. Thanks for all the help, I'll keep you guys updated.


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OfflinePrimal Call
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Re: Concerns about first monotub [Re: NotMyName]
    #14281488 - 04/12/11 11:33 PM (12 years, 10 months ago)

don't turn it off completely... get a timer, and leave the fan in the closet or put it somewhere far enough away that it can stay on 24/7 and slowly move the airflow of the whole room/area.

balance moisture and FAE = win


--------------------
New Cultivator's Guide
Time to fruit? Pinning Strategy and Troubleshooting
My Trade Thread (Fungus, Plants, Herbal Medicine)


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Invisibleanonjon
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Re: Concerns about first monotub [Re: Primal Call]
    #14282438 - 04/13/11 06:44 AM (12 years, 10 months ago)

I think u guys r missing something obvious. Those beige / brown spots don't just look like dry spots, they look like it's contaminated.

Can we get some more macros of those spots? I think that tub is toasted, which makes all this other chatter irrelevent.


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The above post is fictional, hypothetical, or downright nonsensical.


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