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OfflineAjaxx
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why wont my mycelium grow any more in my LCs?
    #14277173 - 04/12/11 09:55 AM (12 years, 10 months ago)

maybe its because i made too large of LCs. this is my first time making LCs. i used about 700-750 mL of water to about 28 grams of karo (4%) and shot up with a little less than 1 cc of pf albino spores per jar.

this is them after a few weeks, maybe even a month after inoculation. the mycelium always hangs out at the bottom, i just had to swirl it to the top to take a picture.


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Offlinek00laid
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Re: why wont my mycelium grow any more in my LCs? [Re: Ajaxx]
    #14277208 - 04/12/11 10:09 AM (12 years, 10 months ago)

myc likes to grow on stuff, not in water


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AMU - AMU Q & A - MyVideo Teks!

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OfflineSynocybin
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Re: why wont my mycelium grow any more in my LCs? [Re: k00laid]
    #14277264 - 04/12/11 10:24 AM (12 years, 10 months ago)

Quote:

k00laid said:
myc likes to grow on stuff, not in water




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Luigi on Shrooms said:
Its been 4weeks since i cased what is going on?
i used human manure(mine) vermiculite and coco coir

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Invisibleyutaka

Registered: 06/12/10
Posts: 544
Re: why wont my mycelium grow any more in my LCs? [Re: k00laid]
    #14277267 - 04/12/11 10:25 AM (12 years, 10 months ago)

What are your lids like?  Do they have a tyvek, polyfil or other filter for gas exchange?  If not, you're suffocating your culture.

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Offlinemagickpsychonaut
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Re: why wont my mycelium grow any more in my LCs? [Re: yutaka]
    #14277277 - 04/12/11 10:28 AM (12 years, 10 months ago)

@Yutaka, I'm a newbie so forgive me if my question sounds dumb, but wouldn't the mycelia be suffocating already since it is submersed in liquid?

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OfflineSynocybin
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Re: why wont my mycelium grow any more in my LCs? [Re: yutaka]
    #14277280 - 04/12/11 10:29 AM (12 years, 10 months ago)

Quote:

yutaka said:
What are your lids like?  Do they have a tyvek, polyfil or other filter for gas exchange?  If not, you're suffocating your culture.




in a way.. this doesnt matter..

he only shot so many spores in the jar, and they have all germinated, there isnt anything he can do now aside from knock up a test jar or refridgerate that lc..

it isnt gonna "grow" anymore until he puts it on spawn...


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Luigi on Shrooms said:
Its been 4weeks since i cased what is going on?
i used human manure(mine) vermiculite and coco coir

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OfflineAjaxx
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Re: why wont my mycelium grow any more in my LCs? [Re: Synocybin]
    #14277338 - 04/12/11 10:44 AM (12 years, 10 months ago)

it has a nail hole in the top of the lid covered with micropore tape.

i shook it up and knocked up some jars, i think ill just take grain liquid cultures from some of them. none of them contaminated.

i guess ill stick to GLC instead of LC since im apparently doing something wrong with my LC. i even followed the tek :shrug:

Edited by Ajaxx (04/12/11 10:48 AM)

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Invisibleyutaka

Registered: 06/12/10
Posts: 544
Re: why wont my mycelium grow any more in my LCs? [Re: Synocybin]
    #14277357 - 04/12/11 10:50 AM (12 years, 10 months ago)

A liquid culture that has proper gas exchange would show better growth than an LC without GE.  It's a pretty weak looking culture compared to some of the ones I've seen with the right GE and stir bars to break up the mycelium.

LC hasn't worked the best for me.  The tek I liked to use was Monster Mitch's LC tek.

Let us know how those jars you knocked up with LC turn out.  :thumbup:

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OfflineSynocybin
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Re: why wont my mycelium grow any more in my LCs? [Re: Ajaxx]
    #14277368 - 04/12/11 10:55 AM (12 years, 10 months ago)

Quote:

Ajaxx said:

i guess ill stick to GLC instead of LC since im apparently doing something wrong with my LC. i even followed the tek :shrug:




I'm about to go postal.. so prepare your ass..

YOU ARE DOING NOTHING WRONG!

there are only so many spores that you shot in that jar.. those spores have germinated and grown to their fullest in that jar.

they will not grow anymore and they will NOT make mushies in the water.

if you dont test it out, you arent going to know whether it'll work or not.

and how about trying something easier, like  just MS'ing your jars?

asking questions then not liking the answeer is a good way to fail in this hobby.

you DID NOT fuck up the LC.. Just realize it doesnt produce spores in the water to take over your water jar.

you arent going to colonize the water.. ever..

stop trying.

make a syringe, and finish your grow.


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Synocybin's Penis Envy Grow Log!
OUTDOOR SPENT TRAY GROW PICS!!
X7X Grow Log

Luigi on Shrooms said:
Its been 4weeks since i cased what is going on?
i used human manure(mine) vermiculite and coco coir

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OfflineAjaxx
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Re: why wont my mycelium grow any more in my LCs? [Re: Synocybin]
    #14277466 - 04/12/11 11:21 AM (12 years, 10 months ago)

Quote:

Synocybin said:
Quote:

Ajaxx said:

i guess ill stick to GLC instead of LC since im apparently doing something wrong with my LC. i even followed the tek :shrug:




I'm about to go postal.. so prepare your ass..

YOU ARE DOING NOTHING WRONG!

there are only so many spores that you shot in that jar.. those spores have germinated and grown to their fullest in that jar.

they will not grow anymore and they will NOT make mushies in the water.

if you dont test it out, you arent going to know whether it'll work or not.

and how about trying something easier, like  just MS'ing your jars?

asking questions then not liking the answeer is a good way to fail in this hobby.

you DID NOT fuck up the LC.. Just realize it doesnt produce spores in the water to take over your water jar.

you arent going to colonize the water.. ever..

stop trying.

make a syringe, and finish your grow.




uh... oook... i didnt not like the answer... idk why youre flippin out. im definitely not failing in this hobby. this was just a test run since ive never attempted an LC before.

i had 50 quarts of GT spawn (lost 2 to contam) so 48 jars spawning to 8 tubs... 5 of the tubs are done, and im putting 3 more GT tubs together tonight and tomorrow.

i knocked up a 11 jars testing out these LCs and they all worked very well with no contamination. im spawning all those jars to a tub tonight/tomorrow also... so ill have 9 monotubs goin...

i guess i didnt know exactly what a liquid culture was... MY BAD.

Edited by Ajaxx (04/12/11 11:23 AM)

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OfflineSynocybin
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Re: why wont my mycelium grow any more in my LCs? [Re: Ajaxx]
    #14277503 - 04/12/11 11:27 AM (12 years, 10 months ago)

its fine dude.

some people just have to realize that time frames dont matter and nothing is ever as it seems with fungi..

things change, things go differently.

and good luck with all of your stuff you have growing, i hope you will update with some pics when they go.


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OUTDOOR SPENT TRAY GROW PICS!!
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Luigi on Shrooms said:
Its been 4weeks since i cased what is going on?
i used human manure(mine) vermiculite and coco coir

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InvisibleFungal growth
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Re: why wont my mycelium grow any more in my LCs? [Re: Synocybin]
    #14277551 - 04/12/11 11:38 AM (12 years, 10 months ago)

idk, i thought the myc would grow untill it ran out
of nutes....ive only made glc though.
but a tiny bit of myc water in an lme lc with
makes mad myc....

y would spores be different?

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Offlinebroken
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Re: why wont my mycelium grow any more in my LCs? [Re: Fungal growth]
    #14277626 - 04/12/11 11:55 AM (12 years, 10 months ago)

Quote:

magickpsychonaut said:
@Yutaka, I'm a newbie so forgive me if my question sounds dumb, but wouldn't the mycelia be suffocating already since it is submersed in liquid?





mycelia breath oxygen like us, but they do not have lungs like us.

Quote:

Fungal growth said:
idk, i thought the myc would grow untill it ran out
of nutes....i





there are several factors that can lead to the stopping of mycelia growth. lack of GE or FAE, temp, lack of H2O in the substrate...


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:willynilly:

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OfflinePerun
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Re: why wont my mycelium grow any more in my LCs? [Re: broken]
    #14277651 - 04/12/11 12:05 PM (12 years, 10 months ago)

Quote:

closed veil said:

Quote:

Fungal growth said:
idk, i thought the myc would grow untill it ran out
of nutes....i





there are several factors that can lead to the stopping of mycelia growth. lack of GE or FAE, temp, lack of H2O in the substrate...




Yeah,but we r talking here about LC,so FAE,lack of H2O(substrate in general),even the GE...cant be an issue!


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First:                                                        Then:

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OfflineSynocybin
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Re: why wont my mycelium grow any more in my LCs? [Re: Fungal growth]
    #14277662 - 04/12/11 12:09 PM (12 years, 10 months ago)

the myc only lives and grows when there are nutes in the water..

try making an LC with just pc'd distilled water. wont work very well..

the nutes help, yes. I use Turbinado LC's and those things explode, but eventually it WILL stop growing..

heres a mathematical equation for you..

if BIlly has exactly 1000 mushroom spores and he puts those into 750 ml of water with a 4% ratio of SiTR or Dextrose or Karo, whathaveyou.

those spores germinate and shot hyphae, when those hyphae find another compatible hyphae the bind and form dikaryotic mycellium, which, since they have no real solid think to grab to and grow stay suspended.

now like i said, he has 1000 spores.. thats all he is ever going to have in that jar becasue thats all he shot into it.  without the proper nutrients or solid movement (ie colonizing grain) they arent going to grow any farther than what they are, because they have already bound with what they can.. they cant grow or bind any further.

considering the small amount of nutes in the water from your sugar, it should take a very short period of time for your LC to reach maturity and eat most all the nutes and STOP growth.

now at this point your LC is dormant, not really dead, just laying in wait of more nutrients, so when spawned they take off like a bat outta hell to try to get the nutes they've missed so much..

most people like using LCs because the cut out the proverbial 2 week germination period and give you straight myc to work with.

its hard to explain to you why your myc isnt growing anymore with out popping a blood vessel in my brain so i'll stop lol

but you get the general idea?


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Luigi on Shrooms said:
Its been 4weeks since i cased what is going on?
i used human manure(mine) vermiculite and coco coir

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Offlinepandelic
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Re: why wont my mycelium grow any more in my LCs? [Re: Perun]
    #14277734 - 04/12/11 12:27 PM (12 years, 10 months ago)

Hyphae will always grow towards the nitruents, Digestive enzymes are produced do take in the nutes.
The Myc should be able to replicate as long as there is nutrients (4% is worked for me for almost a year).
One thing you must consider is that as CV sa id they breathe O2 so there has to be an exchange for the CO2 they breathe out.
I always get better results with LC when  you make them at most half the capacity of the jar.
Your jar should work just fine though.
Hope that helps

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OfflineGreenBlanket
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Re: why wont my mycelium grow any more in my LCs? [Re: pandelic]
    #14277816 - 04/12/11 12:45 PM (12 years, 10 months ago)

I started some lc's on 3/20 and they have similar looking growth. I also had been expecting more growth. If it appears to have stopped growing, should I just remove the tyvek and cover the GE hole with my finger (after sanitizing it) then shake it up before making my syringes?

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OfflineAjaxx
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Re: why wont my mycelium grow any more in my LCs? [Re: GreenBlanket]
    #14277843 - 04/12/11 12:53 PM (12 years, 10 months ago)

Quote:

GreenBlanket said:
I started some lc's on 3/20 and they have similar looking growth. I also had been expecting more growth. If it appears to have stopped growing, should I just remove the tyvek and cover the GE hole with my finger (after sanitizing it) then shake it up before making my syringes?




i shook mine up good before sucking it up into a syringe. get it as evenly distributed as possible then suck it up. theyll colonize jars reeeally fast. its great :smile:

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OfflineSynocybin
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Re: why wont my mycelium grow any more in my LCs? [Re: GreenBlanket]
    #14277859 - 04/12/11 12:55 PM (12 years, 10 months ago)

Quote:

GreenBlanket said:
I started some lc's on 3/20 and they have similar looking growth. I also had been expecting more growth. If it appears to have stopped growing, should I just remove the tyvek and cover the GE hole with my finger (after sanitizing it) then shake it up before making my syringes?




Oh my fucking god does noone fucking read anymore?!?!

this is exactly the fucking reason this website pisses me off and makes me not want to spend time here anymore..

ATTN: NOOBS. READ! USE THE SEARCH FUNCTION, FUCK UP A GROW OR TWO, COME BACK AND ASK QUESTIONS. YOUR UNWILLINGNESS TO KNOW THE FACTS AND COMPLETE DISREGARD FOR STERILE TECHNIQUE IS YOUR OWN FAULT! LEARN EVERYTHING INSIDE AND OUT. DONT GET PISSED BECAUSE "MY MUSHIES ARE ONLY 2 INCHES TALL" GUESS WHAT? THATS YOUR FAULT TOO! YOU ARENT ON HERE TO GROW MUSHIES TO BECOME THE NEXT PABLO FUCKING ESCOBAR OF FUCKING FUNGUS, YOU NEED TO BE ON HERE TO LEARN.

I'm fucking out of here for a couple days.. fuck this stupid shit


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Luigi on Shrooms said:
Its been 4weeks since i cased what is going on?
i used human manure(mine) vermiculite and coco coir

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Offlinequebus
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Re: why wont my mycelium grow any more in my LCs? [Re: GreenBlanket]
    #14277877 - 04/12/11 12:58 PM (12 years, 10 months ago)

What is a GT tub?  Thanks.

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