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ygunz
Stranger

Registered: 04/01/11
Posts: 102
Last seen: 11 years, 5 months
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Contam Again ! Help
#14276789 - 04/12/11 07:33 AM (12 years, 9 months ago) |
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After several good jars seems contam is back. using all fully cleaned stuff.got years of experience but I am surely missing something ,plz help ,any info will help
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Contam Again ! Help [Re: ygunz]
#14276814 - 04/12/11 07:49 AM (12 years, 9 months ago) |
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Quote:
ygunz said: any info will help
Agreed, may we please have some?
-------------------- You make it all possible. Doesn't it feel good?
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DynGBreeD
┌∩┐(◣_◢)┌∩┐


Registered: 01/15/11
Posts: 3,639
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Re: Contam Again ! Help [Re: Doc_T]
#14276840 - 04/12/11 07:58 AM (12 years, 9 months ago) |
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Pictures maybe? Sterilization technique?
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ygunz
Stranger

Registered: 04/01/11
Posts: 102
Last seen: 11 years, 5 months
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Re: Contam Again ! Help [Re: DynGBreeD]
#14276904 - 04/12/11 08:33 AM (12 years, 9 months ago) |
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had some green/black contam, was using open bag of verm (verm/BR)1/2pt jars. boiled ,acohol wipe ,new syringe,baked verm - boiled syringe water - now black contam in 3 sets of jars , must be causing Lc contam all of a sudden ,going nuts trying to figure out,maybe airbourne stuff in apt ?
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Bobthehobo9
Remove Before Flight



Registered: 01/14/11
Posts: 277
Last seen: 10 months, 14 days
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Re: Contam Again ! Help [Re: ygunz]
#14276941 - 04/12/11 08:45 AM (12 years, 9 months ago) |
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go get a PC from walmart or a thrift store. 90 min at 15psi will help you out a ton.
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ygunz
Stranger

Registered: 04/01/11
Posts: 102
Last seen: 11 years, 5 months
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been using 1 for years but at 30 mins @ 15psi - think some how I am screwing up my syringes ?
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darkhawk37
killzor

Registered: 03/17/11
Posts: 93
Last seen: 2 years, 3 months
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Re: Contam Again ! Help [Re: ygunz]
#14277020 - 04/12/11 09:12 AM (12 years, 9 months ago) |
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make sure u got a clean environment to knock jars, a piece of dust could and will land on your needle if you dont swab the tip with alcohol. alot of people build gloveboxs for this i dont think it necessary
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ygunz
Stranger

Registered: 04/01/11
Posts: 102
Last seen: 11 years, 5 months
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Re: Contam Again ! Help [Re: darkhawk37]
#14277025 - 04/12/11 09:14 AM (12 years, 9 months ago) |
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how important is Lysol ?
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darkhawk37
killzor

Registered: 03/17/11
Posts: 93
Last seen: 2 years, 3 months
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Re: Contam Again ! Help [Re: ygunz]
#14277037 - 04/12/11 09:17 AM (12 years, 9 months ago) |
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very important. unless u wanna make a bleach mix, YOU MUST have a clean environment
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ygunz
Stranger

Registered: 04/01/11
Posts: 102
Last seen: 11 years, 5 months
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Re: Contam Again ! Help [Re: darkhawk37]
#14277044 - 04/12/11 09:19 AM (12 years, 9 months ago) |
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racking my brain , can't figure out, in ur opinion PCing should take care of ALL problems in BR/Verm jar ?
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darkhawk37
killzor

Registered: 03/17/11
Posts: 93
Last seen: 2 years, 3 months
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Re: Contam Again ! Help [Re: ygunz]
#14277054 - 04/12/11 09:21 AM (12 years, 9 months ago) |
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yea pcing is a most. you might wanna look into making a glove box for nocking up your jars to
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krum
la muerte peluda


Registered: 07/30/10
Posts: 557
Last seen: 10 years, 5 months
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Re: Contam Again ! Help [Re: ygunz]
#14277070 - 04/12/11 09:25 AM (12 years, 9 months ago) |
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Quote:
ygunz said: got years of experience
in what?
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thenpc
Nomad & Keeper of Fire



Registered: 06/10/10
Posts: 339
Loc: SE USA
Last seen: 2 years, 5 months
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Re: Contam Again ! Help [Re: ygunz]
#14277077 - 04/12/11 09:27 AM (12 years, 9 months ago) |
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LC contam is likely if it's sets of jars but we can't know every variable of your procedure.
-------------------- My Strainlist
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ygunz
Stranger

Registered: 04/01/11
Posts: 102
Last seen: 11 years, 5 months
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Re: Contam Again ! Help [Re: thenpc]
#14277610 - 04/12/11 11:52 AM (12 years, 9 months ago) |
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I must have contamed 2 syringes in a row some how. not gonna say I go to all the cleaniness as some but never had a problem like this since I started (3-4 years)never used glove box but gonna look into it
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
Loc: Scoping Woodchips
Last seen: 11 years, 7 days
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Re: Contam Again ! Help [Re: ygunz]
#14277854 - 04/12/11 12:55 PM (12 years, 9 months ago) |
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Why are you still doing BRF if you have a pressure cooker? Dude inoc some grain jars You get way more spawn per ml of spore solution (a qt of spawn opposed to a 1/2pint) and if you prep it right then you wont have any bursted grains and it will be fine. Im out to buy a PC, some silicone, and some rye today so i can inoc some qt jars.
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Wavy Cap Cyan Spore Prints for Trade/Sale
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