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OfflineTrippy_Smurf
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Bunk syringe?
    #14273848 - 04/11/11 05:46 PM (12 years, 10 months ago)

I recently got 2 syringes of PE6 from a vendor.

I haven't opened one, but with the other, I noc'd up 4 rye jars, 2 MEA plates, 5 LCs, and a couple PFs.

That was 2 weeks ago and until today, there was no sign of anything from the rye or LCs.  The agar has been growing, but I thought it was looking a little pink.

Today I noticed that 4 of the LCs had whisps of white floating around the bottom.  Upon closer examination, I found that the white as actually sediment on the bottom that got stirred up when I moved the jars.  The 5th lc had the same sediment, but it was pinkish.

I then took a good look at the plates and one of them looks very red.

I'm guessing a bacterial contam?



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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14273884 - 04/11/11 05:53 PM (12 years, 10 months ago)

Quote:

I'm guessing a bacterial contam?




:thumbup: by which I mean :thumbdown:

Hard to see in the pics- is there any myc you can transfer out of the dishes?


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OfflineTrippy_Smurf
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Re: Bunk syringe? [Re: Doc_T]
    #14273909 - 04/11/11 05:57 PM (12 years, 10 months ago)

Hard to say... I don't know exactly what I'm looking for.  Everything looks pink/red though.


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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274107 - 04/11/11 06:32 PM (12 years, 10 months ago)

Nothing fluffy and while like cotton or kite string?

Contact the vendor, explain that your microscopy slides were contaminated with bacteria and may you please have a replacement.


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OfflineTrippy_Smurf
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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274144 - 04/11/11 06:39 PM (12 years, 10 months ago)

I opened the plate that had the lesser amount of growth and the splotch in the middle was a pink liquid with no rhizoporphic growth at all.  when I tilted the plate to the side and shook it, all of the pink ran off.  If there was any myc in there, it'll come out when I write my "Garbage Disposal Tek".

I tried to get some better pictures of the remaining plate...



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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274196 - 04/11/11 06:49 PM (12 years, 10 months ago)

Sorry about your fail man, goes to show that you should always start with agar.

Then again, most big name vendors can be trusted, but everyone, at some point, will ship contaminated syringes. It's how they handle it that really counts. Which vendor was this, if you don't mind me asking?


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Re: Bunk syringe? [Re: afrosheen]
    #14274219 - 04/11/11 06:52 PM (12 years, 10 months ago)

Quote:

afrosheen said:
Sorry about your fail man, goes to show that you should always start with agar. 




Yeah, aren't you glad you didn't make LC with this?


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OfflineTrippy_Smurf
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Re: Bunk syringe? [Re: afrosheen]
    #14274224 - 04/11/11 06:53 PM (12 years, 10 months ago)

Since this is a shit syringe, I'd rather not say.

I'll do up another batch of agar and test the other syringe and then contact him after I know what's up with that one.


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Re: Bunk syringe? [Re: Doc_T]
    #14274230 - 04/11/11 06:54 PM (12 years, 10 months ago)

LOL Doc, he did make LC's, 4 to be exact. :popcorn:


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OfflineTrippy_Smurf
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Re: Bunk syringe? [Re: Doc_T]
    #14274238 - 04/11/11 06:55 PM (12 years, 10 months ago)

Quote:

Doc_T said:
Quote:

afrosheen said:
Sorry about your fail man, goes to show that you should always start with agar. 




Yeah, aren't you glad you didn't make LC with this?




I did... I made 5 pints.

I'll probably start an LC or two in parallel with the agar on the next one also, just because I'm trying to get more going and my agar skills are still sub-par.  However, I'll wait till I'm sure the agar is good before I use the LC.


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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274242 - 04/11/11 06:56 PM (12 years, 10 months ago)

LC blows.
Get a clean agar culture, transfer to grains, then make grain LC.


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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274252 - 04/11/11 06:57 PM (12 years, 10 months ago)

I'm also going back to karo LC... I made a LME LC for the biopsy of my first fruit and it's way dark and sedimenty.


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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274264 - 04/11/11 06:59 PM (12 years, 10 months ago)

I have much more confidence in my LC skillz than my agar skillz.  Also, it's extremely difficult to see anything in a 1/2 pint when you can't get a good, clear agar with no condensation.

At this point, to me, agar is more of a toy than a tool.


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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274272 - 04/11/11 07:00 PM (12 years, 10 months ago)

LME > karo.

Why are you getting condensation, are your jars sealed up?


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Re: Bunk syringe? [Re: Doc_T]
    #14274310 - 04/11/11 07:05 PM (12 years, 10 months ago)

He might be pouring too hot in a cool room. Condensation should dry up somewhat as long as the dish/jar can breathe.


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Re: Bunk syringe? [Re: afrosheen]
    #14274381 - 04/11/11 07:22 PM (12 years, 10 months ago)

How long should I PC my LC and agar?

I did both for 30 at 15psi and the agar came out darker than I was expecting and the LC came out much darker than expected, to the point that I have to hold it up to the light and really look to even see the marbles.


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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274397 - 04/11/11 07:26 PM (12 years, 10 months ago)

20-30 mins. My lc tek is in my journal if you want to look.


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Re: Bunk syringe? [Re: Doc_T]
    #14274441 - 04/11/11 07:38 PM (12 years, 10 months ago)

I'll have to try 20 minutes next time.  Where do you get dextrose?  Is that something a home brew supply store would have?  I looked around when I got my LME, but didn't see any.  I would have asked, but I was really, really baked.

What recipe do you use for agar?


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Re: Bunk syringe? [Re: Trippy_Smurf]
    #14274481 - 04/11/11 07:46 PM (12 years, 10 months ago)

Agar recipe: 500ml distilled water, 10g light malt extract, 10g agar agar strips (or powder, or whatever). Bring water and agar to a rolling boil, add LME, reduce heat and continually stir until it looks right. Should take no longer than 10 minutes once the water boils.

LME is at brew stores, dextrose is at those emo health food stores. It's basically a type of powdered sugar. Malt dextrose should be at brew stores also.


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Re: Bunk syringe? [Re: afrosheen]
    #14274537 - 04/11/11 07:58 PM (12 years, 10 months ago)

I have LME.  No dex though.  However, since karo ~ dex, couldn't I substitute?


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