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OfflineTerry M
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Registered: 06/18/10
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Plate made from center of Pn plate. Is it also Pn?
    #14272637 - 04/11/11 04:18 PM (10 years, 1 month ago)

This is a not an angels-on-the-head-of-a-pin question, but I think a legitimate one. If taking a square some cultivator-defined distance from the center of a Pn plate makes a P(n+1) plate, then what if I take a square right AT the center, where the original culture started on that plate?

I'm actually in such a situation because I have an important plate with contam at the edges that I would like to save. I've already made a bunch of P2s from it, plus a "center transplant" because the plate is going to die. Since the original mycelium hasn't grown out from its location, can't this center transplant be consider of the same generation for senescence purposes? Or am I missing something?


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OfflineRalafe
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Re: Plate made from center of Pn plate. Is it also Pn? [Re: Terry M]
    #14274617 - 04/11/11 10:17 PM (10 years, 1 month ago)

I am interested in learning more about this, myself :strokebeard2:


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OfflineRogerRabbitM
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Re: Plate made from center of Pn plate. Is it also Pn? [Re: Ralafe]
    #14274623 - 04/11/11 10:18 PM (10 years, 1 month ago)

No, it's the same age.  The culture is one organism.
RR


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Invisiblemycoelf
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Re: Plate made from center of Pn plate. Is it also Pn? [Re: RogerRabbit]
    #14274791 - 04/11/11 10:49 PM (10 years, 1 month ago)

on agar senescence is 150+ generations of agar plates out form a multi spore germination/clone F1. Don't sweat senescence, just make a master slant culture(s) and when you have made ten to 20 generations of plates descended from a master return to the source ans start over. Provided the fact of multiple daughter plates from any given mother culture can all give rise to spawn runs of their own I do not but once a year at best make 10-20 generations of plates in one growing season.:goodluck:


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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