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Offlinejesus666
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can somebody explain me that?
    #14270812 - 04/11/11 06:26 AM (12 years, 9 months ago)

hi folks.... i was bored so i tried to make some pf jars and sterilize them. I let them sit in the pot with the lid with still alluminium foil for more than a month...

last week i checked them three and i see only one was still "safe" the other two were almost entirely green. Today i check them again and the one highlander became green too.

I can't explain this. After 90'+ boiling shouldn't they be completely sterile? why one of them one week ago was still clean and now a mess? jars are covered with lids, tyvek and aluminium foil. They hadn't been inoculated...


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InvisibleDoc_T
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Re: can somebody explain me that? [Re: jesus666]
    #14270876 - 04/11/11 06:50 AM (12 years, 9 months ago)

Not quite sterile. Now you know why we use pressure cookers.


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OfflineRogerRabbitM
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Re: can somebody explain me that? [Re: Doc_T]
    #14270960 - 04/11/11 07:33 AM (12 years, 9 months ago)

None of it was sterile once the water cooled down and contaminant spores entered the vessel.  With saturation humidity like that, it's no wonder mold spores found their way in.
RR


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OfflineSomeGuy
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Re: can somebody explain me that? [Re: Doc_T]
    #14270968 - 04/11/11 07:36 AM (12 years, 9 months ago)

Quote:

Doc_T said:
Not quite sterile. Now you know why we use pressure cookers.



now you know why you shouldn't sterilize too much in advance. Wait until you are ready to inoculate before sterilizing your substrate


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Offlinejesus666
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Re: can somebody explain me that? [Re: SomeGuy]
    #14271014 - 04/11/11 07:57 AM (12 years, 9 months ago)

so if i understood... high umidity range can bring mold spores also through alluminium, lid and tyvek???

in fact a friend of mine usually put his jars to incubate in a tub with water and an acquarium heater, jars' bottom touching water surface.
he has always a contam rating really, really high...


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OfflineSomeGuy
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Re: can somebody explain me that? [Re: jesus666]
    #14271079 - 04/11/11 08:15 AM (12 years, 9 months ago)

you can't sterilize something 100%, even by pressure cooking. The basic idea is you kill enough of the contaminant/stunt it so hard, that the mushroom has a chance to outgrow it, and colonize all the food-source. A pf cake, uninnoculated, will eventually start growing contamination, even if it's 100% sealed


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Offlinetotal
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Re: can somebody explain me that? [Re: RogerRabbit]
    #14271450 - 04/11/11 09:53 AM (12 years, 9 months ago)

Quote:

RogerRabbit said:
With saturation humidity like that, it's no wonder mold spores found their way in.
RR



:whathesaid:
You let them sit in a pot of water for a month...
What did you expect to happen? :shrug:
Standing water is a breeding ground for all things bad...
Even leaving jars in an unopened pressure cooker for that long will likely contam..


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Offlinejesus666
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Re: can somebody explain me that? [Re: total]
    #14272533 - 04/11/11 01:55 PM (12 years, 9 months ago)

yeah but there's no way for water to penetrate foil and tyvek over all


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OfflineSomeGuy
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Re: can somebody explain me that? [Re: jesus666]
    #14272544 - 04/11/11 01:58 PM (12 years, 9 months ago)

Quote:

jesus666 said:
yeah but there's no way for water to penetrate foil and tyvek over all



thats why you know it started inside the cake, and what I said earlier is true, I didn't make it up


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Offlinetotal
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Re: can somebody explain me that? [Re: jesus666]
    #14272545 - 04/11/11 01:58 PM (12 years, 9 months ago)

:smbfacepalm:


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InvisibleFungal growth
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Re: can somebody explain me that? [Re: jesus666]
    #14272548 - 04/11/11 01:59 PM (12 years, 9 months ago)

Quote:

jesus666 said:
yeah but there's no way for water to penetrate foil and tyvek over all



does'nt matter. as was said, it was never COMPLETELY
sterile. those few contams left will eventually
recover and germinate.
try again.


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OfflineMad River
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Re: can somebody explain me that? [Re: Fungal growth]
    #14272585 - 04/11/11 02:08 PM (12 years, 9 months ago)

Jebus just doesn't want you to grow mushrooms.


Edited by Mad River (04/11/11 02:10 PM)


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