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Offlinetriond
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Registered: 04/22/10
Posts: 31
Last seen: 2 years, 4 months
weird mycelium
    #14269084 - 04/10/11 08:39 PM (12 years, 9 months ago)

hi
i have done above 3 time  ps. cubensis culture without any problem
from A to Z
but now i have tried someone culture and one was a disaster major contamination
(look like trichoderma)
thinking that is because of the reused syringe
and that syringe was poorly sterilized
i was thinking the syringe  was responsible

but now i have started a new batch
and there something that look like contamination
even if i have changed the syringe and the needle
do that is because of the air is contaminated or the print
:ooo:
bye

(note i use bottled water.. on the bottle they say its osmosed and ozoned)
maybe i should use sterile water from the pharmacy)


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InvisibleSlimz
.-~*´`*·~-experience-~*´`*·~-.
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Registered: 10/03/07
Posts: 3,588
Loc: Maryland
Re: weird mycelium [Re: triond]
    #14269105 - 04/10/11 08:42 PM (12 years, 9 months ago)

what tek are you using to make your syringe? sounds like you have a sterilization issue..


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Offlinetriond
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Registered: 04/22/10
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Last seen: 2 years, 4 months
Re: weird mycelium [Re: Slimz]
    #14269447 - 04/10/11 09:34 PM (12 years, 9 months ago)

i use popcorn tek
last time when its worked  i sterilized the substrate jars it in a pressure cooker for 40 minute

my syringe come from  a sterile package  .. needle are also come from  sterile package


some of my friend tested my spores print and he have no issue

i clean the needle each time with Lysol
(not sure if putting needle on a flame will melt the support)

i use a metal cup  that i sterilise with Lysol for 10 minute


normally i use the tip of a needle to take of the spores from the print ..  into the  metal cup until there is splendid of spores
and i put water from a syringe )

and i place a plastic cover on the metal cup until i need it  and i replace the cover each time i don't need it.

when i inject i clean the spot where i put the needle each time

i don't know what i doo wrong but its cause a massive contamination

if need more info about how i do just ask.
bye


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