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kotter


Registered: 01/15/11
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Earth's Tongue's Hericium 'americanum'
#14267640 - 04/10/11 03:35 PM (12 years, 9 months ago) |
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A while back someone mentioned that they never could get this ET Hericium to fruit. I have had a spawn bag colonized for some time that appears to *want* to fruit but does not. I'm being patient. Where I encountered something odd, considering that, is I used the same spore syringe that I used to inoculate their prepared spawn bag and used the rest of it for LC. I more recently inoculated more spawn bags (mine not theirs) from that LC that are colonizing nicely. I also did some PDA and some oatmeal dextrose agar plates from it and ran into some time conflicts so did not get back to a stack of them as fast as I should have. Today when I did, I found this (several instances actually but I'm shooting through plastic so the images are crappy and as a result I'm only posting one until I can take a photo in the flow hood)

Clearly ET's "americanum" has the ability to fruit. Or at least what was on plates was able to. Why it did not do so in their prepared media I do not know but wanted to share this experience in hopes that someone can help me understand it? I'll add the results on the bags that I did more recently whenever is something more than just colonization.
Edited by kotter (04/11/11 07:13 PM)
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kotter


Registered: 01/15/11
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Re: Earth's Tongue's Hericium "americanum" [Re: kotter]
#14272382 - 04/11/11 01:24 PM (12 years, 9 months ago) |
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Some more images. Another series of plates and a slant from the cleanest portions of the mycelium from these was done today. More details when or if something else happens. Still no fruiting on anything but the plates.

Plate 2 was on PDA, plates 1 & 3 on the same medium but with gentamycin added, plate 4 was on ODA.
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kotter


Registered: 01/15/11
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Re: Earth's Tongue's Hericium "americanum" [Re: kotter]
#14419856 - 05/08/11 02:31 PM (12 years, 8 months ago) |
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Its about a month later and the spawn bag is almost unchanged. I thought I'd post an image of it. Anyone have an idea why a stack of agar plates fruited but the bag has not. All came out of the same syringe. (I did another set of plates using the plate that fruited.) A month and a bit ago the bag had a slit made as suggested. I'm probably more curious as to why that agar plate fruited than why the bag has not.
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RogerRabbit
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Re: Earth's Tongue's Hericium "americanum" [Re: kotter]
#14422018 - 05/08/11 09:38 PM (12 years, 8 months ago) |
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It might help to say what media is in the spawn bag. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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kotter


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Re: Earth's Tongue's Hericium "americanum" [Re: RogerRabbit]
#14422599 - 05/08/11 11:38 PM (12 years, 8 months ago) |
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I could not agree more but it came made up so I lack a meaningful answer. It had a bunch of stuff in it that was supposed to be broken up into small pieces once inoculated. It looked like mixed straw and wood chips in some sort of odd sandwiching when it first arrived. This was the very first spawn bag to touch my hands ever. (Back in December.) More recently I've done some tanoak and tanoak/bran using a liquid culture from the same syringe- they colonized faster and I hope will fare better.
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Versicolor
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Re: Earth's Tongue's Hericium "americanum" [Re: kotter]
#14423545 - 05/09/11 08:13 AM (12 years, 8 months ago) |
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I find this very interesting. Lions mane is a woodloving mushroom, so a sawdust substrate is the way to go. I am reading the Paul Stamets book 'growing gourmet and medicinal mushrooms', and I found an interesting quote on the lions mane fruiting on plates.
Quote:
If the top and bottom of the culture dishes are taped together, evaporation is lessened with an associated pooling of carbon dioxide. This stimulates the mycelium into aerial growth. Young fruitbodies form in petri dish cultures incubated at 75°f (24°c)In 2 to 3 weeks.
And this too
Quote:
The mycelium grows relatively slowly on agar media, with fruitbodies often forming before the mycelium has grown to a mere 2.5cm in radius. Furthermore, the transferring of mycelium from agar to grain using the traditional scalpel and wedge techniche results in comparably slow growth.
I will post more on how to get it to fruit in bags later.
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kotter


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Re: Earth's Tongue's Hericium "americanum" [Re: Versicolor]
#14423845 - 05/09/11 10:11 AM (12 years, 8 months ago) |
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Thanks for that. It explains much. I had read that work but somehow missed those comments when doing so. It seems to like the tanoak in bags. Any advice on fruiting is hugely welcomed.
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MonkeyKnifeFight
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Re: Earth's Tongue's Hericium "americanum" [Re: kotter]
#14424250 - 05/09/11 11:52 AM (12 years, 8 months ago) |
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I've got some h americanum growing out and so far it is indistinguishable from h erinaceus. If that's the case then fruiting is dead simple. Just let it fully colonize, wait a week or so after and then make 3 or 4 little slits in the bag and it'll fruit out of those. I just had a erinaceus bag fruit right through the filter in the bag and grow a single massive fruit. It's hard to mess these guys up.
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kotter


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Thanks for that. Yes, americanum is synonymous with erinaceus.
I'll stay patient. Its been some weeks since I cut a slit in the bag. I've added a couple more slits now. I just did the same thing with the other bags I had inoculated more recently (and which are now nicely colonized) so if its just a substrate thing I should be able to know soon. I think that I'm going to buy a different strain of erinaceus and see if I have different results.
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MonkeyKnifeFight
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Re: Earth's Tongue's Hericium "americanum" [Re: kotter]
#14435043 - 05/11/11 01:53 PM (12 years, 8 months ago) |
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Quote:
kotter said: Thanks for that. Yes, americanum is synonymous with erinaceus.
Not that I've seen. Every source I see says these are two distinct species. Obviously similar macro characteristics but I see no indication that americanum and erinaceus are synonyms.
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kotter


Registered: 01/15/11
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Thanks for that welcomed clarification. I'm not sure where I got that from as the source where I thought I'd read that doesn't say it. The photos I've seen look different from each other (online photos are what led me to buy it back in December) so I really don't know offhand where that more recent thought of mine came from. It was probably just another hallucination from the spirochetes in my brain (my CNS is currently being joyridden by lyme et al.). If so my apologies, if I do manage to locate where I read that I'll add a comment but doubt I will. Real hallucinations are annoyingly a persistent part of my reality anymore.
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kotter


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Re: Earth's Tongue's Hericium 'americanum' [Re: kotter]
#14944522 - 08/18/11 08:55 PM (12 years, 5 months ago) |
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I wanted to follow up on this surprisingly old post and say the problem appeared to be with ET's bagged substrate not with their spores. Using sawdust augmented with gypsum (as per RR's video on sawdust but with no other amendment and adding the water a bit on the dry side) the bags all seem to be colonizing nicely and the first two I did are fruiting meagerly. I've gotten two flushes of single fist sized fruiting bodies and one about half that off one and this from the other. Still nada from the ET bag despite it colonizing.


Beautiful organism and fun to grow even if slow, this one shown above had a nice taste going in (sauteed in butter) but so far all have had an intensely bitter finish to the flavor and the first were tough and bitter from the start. Does anyone have experience with that?
I'm also having some trouble posting images right apparently. Apologies about that while I figure out what I am doing wrong.
Edited by kotter (08/18/11 09:02 PM)
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RogerRabbit
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Re: Earth's Tongue's Hericium 'americanum' [Re: kotter]
#14944986 - 08/18/11 10:45 PM (12 years, 5 months ago) |
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It sounds like you got a degraded strain. Lion's mane is normally very fast, fruiting sometimes in less than 30 days from inoculation. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Aleon
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Re: Earth's Tongue's Hericium 'americanum' [Re: RogerRabbit]
#14945188 - 08/18/11 11:59 PM (12 years, 5 months ago) |
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Yeah sounds really slow, i agree with RR. Try a new strain and c what happens. I normally put my LM blocks in 2 weeks after inoc (10% inoc rate), and they start fruiting a few days after. Good luck, lions mane is a great medicinal, and it tastes like seafood!
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kotter


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Re: Earth's Tongue's Hericium 'americanum' [Re: Aleon]
#14946297 - 08/19/11 08:26 AM (12 years, 5 months ago) |
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I totally love the flavor of every Hericium I've tasted -- other than this one. (Amazingly I'm still eating wild lion's manes I cooked and froze to capture last Winter's incredible abundance) I have to wonder if the bitter taste and poor performance is why more people don't grow H. americanum or if, as RR suggests, this one in my hands is just a poor strain. Its been a lot of fun to watch anyway. The first fruit from the straight sawdust can be seen at another post here so its clear even its rate of expansion is slow for a hericium: http://www.shroomery.org/forums/showflat.php/Number/14522365
Thanks for all of the input. Slow is polite. The first bag (my very first one ever) was inoculated somewhere around 8 months ago using part of a spore syringe, colonized great and has never fruited at all. Still no idea what media was in that one but it was the only (and last) preprepared spawn bag I've ever purchased so its moot at this point.
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kotter


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Re: Earth's Tongue's Hericium "americanum" [Re: RogerRabbit]
#15235245 - 10/16/11 06:50 PM (12 years, 3 months ago) |
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Sorry for such a delayed reply to this but I only today do I have any clue.
That original commercial spawn bag (my first spawn bag and my only preprepared bag) never did fruit. I cut it open today to see what was in it and it seemed to be some sort of straw or other grass parts mixed with vermiculite. If there was wood there it seems to have been eaten but Hericiums I've seen in the wild leave a characteristic fragile remnant they don't eat.



This seems to do OK on sawdust or sawdust with a little bran (with gypsum in both cases).
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