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Offlinedeniks
Alien
I'm a teapot


Registered: 01/29/11
Posts: 245
Last seen: 6 years, 10 months
contamina
    #14265194 - 04/10/11 12:51 AM (12 years, 9 months ago)

I'm having a big problem lately,every jar I make start's smelling in a week or so then nothing grows at all(probably bacteria).I have now narrow down to two possible ways of infection,water or spore solution since I was the one making print's,me and my friend decided to make a new batch of jars with sterilized water,vermiculite,jars...everything was pre-sterilized before final pressure cooker sterilization,except food(flower).Evan the syringes wore made brand new and of a different spore print.Also I used my previous spore solution on some agar plates and it worked just fine.If this doesn't work(it better does),my next step is to use liquid culture on jars,thus accelerating growth rate so that mycelia can overcome bacteria.

Any other thoughts on this would be well appreciated.

Thanky,Denisk


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Offlinedeniks
Alien
I'm a teapot


Registered: 01/29/11
Posts: 245
Last seen: 6 years, 10 months
Re: contamina [Re: deniks]
    #14265198 - 04/10/11 12:53 AM (12 years, 9 months ago)

Her's a pic of my new jars


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OfflineHumility
Working on it
 User Gallery


Registered: 10/07/08
Posts: 6,745
Last seen: 6 years, 11 months
Re: contamina [Re: deniks]
    #14267877 - 04/10/11 04:42 PM (12 years, 9 months ago)

Check out some of the teks on here for making clean sporeprints.

It can be difficult but it's a staple of mycology.  You're making strides brother.


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