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OfflineTheBoomking
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Cloning from bulk spawn
    #14251926 - 04/07/11 08:02 AM (12 years, 10 months ago)

So I'm wondering how/if it would be possible to clone from spawn bags that are currently colonizing... should i just bust em open in a glove box? and transfer to jars? wait till they;re done and do it right before fruiting? (using WBS and straw) i want to make syringes from my straw bags, or just clone to a jar. I dont want this to be the last of my oysters!

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Offlinehappihourhero
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Re: Cloning from bulk spawn [Re: TheBoomking]
    #14252068 - 04/07/11 08:54 AM (12 years, 10 months ago)

Both sound good in theory. Give it a shot and keep everyone posted.

Edited by happihourhero (04/07/11 10:08 AM)

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Offline3n1gm4
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Re: Cloning from bulk spawn [Re: happihourhero]
    #14252095 - 04/07/11 09:02 AM (12 years, 10 months ago)

I thought you had to take a tissue sample from the inside of the stem of a live mushroom and put it in a LC or agar plate?

I don't think that GTG is cloning, it is just transferring the already growing myc to another food source.

You could PC a grain jar and put a piece of it in there, then if it fully colonizes with no contams make a GLC from that and you have enough live myc to fill 50, 10cc syringes. 

Take a print:shrug:

Edited by 3n1gm4 (04/07/11 09:06 AM)

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Offline3n1gm4
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Re: Cloning from bulk spawn [Re: TheBoomking]
    #14252131 - 04/07/11 09:08 AM (12 years, 10 months ago)

Quote:

TheBoomking said:
So I'm wondering how/if it would be possible to clone from spawn bags that are currently colonizing... should i just bust em open in a glove box? and transfer to jars? wait till they;re done and do it right before fruiting? (using WBS and straw) i want to make syringes from my straw bags, or just clone to a jar. I dont want this to be the last of my oysters!




Wait till they are fully colonized.


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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InvisibleDoc_T
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Re: Cloning from bulk spawn [Re: 3n1gm4]
    #14252197 - 04/07/11 09:30 AM (12 years, 10 months ago)

Are we talking about a bag of grain? And then doing grain to grain transfer into other bags?


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You make it all possible. Doesn't it feel good?

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Offline3n1gm4
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Re: Cloning from bulk spawn [Re: Doc_T]
    #14252259 - 04/07/11 09:50 AM (12 years, 10 months ago)

Quote:

Doc_T said:
Are we talking about a bag of grain? And then doing grain to grain transfer into other bags?




I believe he was going to xfer some grain from a bag not fully colonized, and taking a grain covered in myc from the less than 100% bag and xfering it into jars for either a LC or grains for a GLC and he thinks that is cloning. Gtg isn't cloning is it? You have to take a plug from a stem to clone right?


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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Offline3n1gm4
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Re: Cloning from bulk spawn [Re: 3n1gm4]
    #14252288 - 04/07/11 09:58 AM (12 years, 10 months ago)

Well the main thing in any GTG is letting it fully colonize so you can visually inspect it for contams, I don't know, if you had a good flow hood you might be able to open it before it was 100% but how are you going to get it sealed back up? You gonna put your impulse sealer in the GB? You have it twist tied?

I need more info to help you with anything except telling you that opening a colonizing bag isn't a good idea.


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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InvisibleDoc_T
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Re: Cloning from bulk spawn [Re: 3n1gm4]
    #14252372 - 04/07/11 10:18 AM (12 years, 10 months ago)

Agreed about full colonization before transfer.
One option you could use earlier would be to get a pack of agar and make  a batch of dishes.
Then you can grab a grain or two in a glovebox and propagate from that.


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You make it all possible. Doesn't it feel good?

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Invisibleanonjon
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Re: Cloning from bulk spawn [Re: 3n1gm4]
    #14252486 - 04/07/11 10:50 AM (12 years, 10 months ago)

Quote:

3n1gm4 said:

I need more info




Yes you do, so why don't you talk less and read more instead of giving your half assed 2 cents all the time.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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InvisibleDoc_T
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Re: Cloning from bulk spawn [Re: anonjon]
    #14252625 - 04/07/11 11:25 AM (12 years, 10 months ago)

Well where's the fun in that? :rolleyes: :lol:


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You make it all possible. Doesn't it feel good?

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Invisiblebiologys
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Re: Cloning from bulk spawn [Re: Doc_T]
    #14252729 - 04/07/11 11:50 AM (12 years, 10 months ago)

anon is back :super::lmafo:

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Offline3n1gm4
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Re: Cloning from bulk spawn *DELETED* [Re: anonjon]
    #14253545 - 04/07/11 02:43 PM (12 years, 10 months ago)

Post deleted by 3n1gm4

Reason for deletion: anonjon doesn't deserve anymore off topic suggestions on keeping him from getting banned, he will make his own bed



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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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Offlinebasic360
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Re: Cloning from bulk spawn [Re: 3n1gm4]
    #14253716 - 04/07/11 03:11 PM (12 years, 10 months ago)

lol @ wall of text and kid on the internet trying to sound educated.  keep spouting off what everyone else says as your own advice and we will keep watching grow after grow fail for you.  perhaps you should not jump from one thing to another like a monkey on adhd and people would take you a little more seriously

as for the transferring, ive never had a problem open air grain to grain or making LCs, but then again my subs dont do too well half the time.  i would wait until the bags are complete and then spoon out into new jars :smile:

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Invisibleanonjon
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Re: Cloning from bulk spawn [Re: 3n1gm4]
    #14254571 - 04/07/11 05:45 PM (12 years, 10 months ago)

Quote:

3n1gm4 said:
There was no reason for that, keep it up so you can get banned again please. It was soo much nicer having one less idiot that thinks he knows everything posting off topic harassment here, so I could learn instead of putting up with childish nonsense and I think my 2 cents are worth quite allot, I care not what you think.

I do not care what you nor any other person that isn't here to learn and makes rude comments thinks about me. If you want to humble yourself let bygones be bygones then man up and quit acting like a spoiled little teen that gets off on being a total jerk, and start trying to act like a TC that answers all questions even if they are stupid questions7, then I will quit taking screenshots of your harassment and flaming and saving them in a folder. If you want to be a jerk and get perma banned then go ahead, you don't bother me, just warning you. AND I KNOW YOU DON'T CARE! Start to....

PS This is the last time I will correspond with you unless you start to act civil, if you were one of my buds and I knew you we messing with me it would be a different story, but I don't know you and I will defend myself even if it is from an online stranger, hopefully you leave me alone and I don't have to do it the mean way. Be good man, I have nothing against you but I will not be demeaned in any fashion.




Oh noes. Please don't tell on me again.

:blowme: :flipthebird:  :crymeariver:

You're the one who admitted you're giving out worthless advice with no experience behind it. I just agreed with you.

Now you're threatening to tattle again. How amusingly childish. I like it.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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OfflineTheBoomking
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Re: Cloning from bulk spawn [Re: anonjon]
    #14262240 - 04/09/11 09:03 AM (12 years, 10 months ago)

yeah I'm going to wait till its fully colonized, I was just wondering if my theory was sound.  and yeah i think ill transfer to jars to make LC then to syringe. Also, chill pills are highly recommended! not really, I recommend herbal remedies.

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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Cloning from bulk spawn [Re: TheBoomking]
    #14262281 - 04/09/11 09:17 AM (12 years, 10 months ago)

Quote:

TheBoomking said:
Also, chill pills are highly recommended! not really, I recommend herbal remedies.




I prefer both together actually. :jah:


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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InvisibleMr. Anderson
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Re: Cloning from bulk spawn [Re: anonjon]
    #14262317 - 04/09/11 09:40 AM (12 years, 10 months ago)

:ilold: :laugh2:


--------------------


Disclaimer: All posts are completely fictional and or for educational purposes only.

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Offline3n1gm4
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Re: Cloning from bulk spawn *DELETED* [Re: anonjon]
    #14265894 - 04/10/11 07:59 AM (12 years, 10 months ago)

Post deleted by 3n1gm4

Reason for deletion: No sense in giving the uncivilized natives skittles..



--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Cloning from bulk spawn [Re: 3n1gm4]
    #14265907 - 04/10/11 08:03 AM (12 years, 10 months ago)

There should be a rule against giving second-hand advice.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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Offline3n1gm4
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Re: Cloning from bulk spawn [Re: anonjon]
    #14266038 - 04/10/11 09:04 AM (12 years, 10 months ago)

Quote:

anonjon said:
There should be a rule against giving second-hand advice.




Everyone here gives second hand advice, unless you are a pioneer or experiment and invent a good tek, you think you are the only one allowed to answer a post? Grow up man and stop acting like you are the fly's turd.



Quote:

anonjon said:
Quote:

3n1gm4 said:

I need more info




Yes you do, so why don't you talk less and read more instead of giving your half assed 2 cents all the time.


Unnecessary just like every time you reply to my posts, take a :chillpill: and some :cubie: s, and smoke a  :bigjoint: or just take the non mycology text elsewhere.(PUB)


I didn't do anything to you, you did it to yourself and when the admins read your posts and the flaming thread you started, you will probably be banned again because you just continue the off topic middle school like behavior, like a wise man said the last time you were banned, good riddance to bad rubbish. I tried to talk to you civilly but now I no longer speak after your rude bantering idiotic replies to my mycology questions. I will just take a screenshot and add it to my collection and then laugh when I see that an admin read your rude replies to many mycology questions on the cult forum and bans you again.

If you were smart you would go erase them all like I am about to do because this is the cult forum and they will ban you for being a jerk to other members for no reason, its in the rules. Once again, trying to help someone who won't listen, and probably wouldn't understand anyway, because I asked politely last time to leave me alone and right after you get back from a ban you start doing the same thing you were banned for. Go to the pub and harass people, I am trying to learn here along with others. keep it up karma will get you eventually. Good luck, hope you chill and learn something instead of getting banned.

Do what you do, cuss me and put me down every time you see me post or grow up. I don't really care I will just ignore you instead of trying to help you from now on.
________________________________________________________________
Check this out-

Barely gets warm with a 13W bulb in it, Then you could paint the tub or tape it to keep in light, and then use one of these on your bulk spawn, just GLC it and shoot it into a magnetic stir LC jar, sterilize the jar of water first. Just do it like the one on amu's first page and you will have 500CCs of thick myc laden water and then you can make all those into more GLC if you want to have tons of fast growing spawn from your one jar.



This might help if you just want to keep the grow going, don't feel like reading back through all the rude off topic comments so if you were talking about cloning a stem disregard this.

I just made 3 quarts rye for GLCs like this and I got 3half pints ELME LCs made with the GLC, noced up a ton of cakes that are growing like crazy, and 7 more grain jars that are growing fast and strong too. And I still have 2 10cc syringes full left that I am making 13 more cakes with today. The LCs I made from it are doing really nice too.

Do a GTG transfer, let that jar fully colonize and then make 500 BRF cakes out of it. Just suggestions because from your question I am not sure if it was cloning you were speaking of. http://www.shroomery.org/forums/showflat.php/Number/11533629#11533629


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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