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Swwert
Truck-Boat-Truck

Registered: 04/08/11
Posts: 1,182
Last seen: 2 years, 10 months
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1st BRF PF Golden Teacher Grow
#14261253 - 04/08/11 11:25 PM (12 years, 10 months ago) |
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Hello all, I'm a long time lurker but recently decided to join!
After a long time of reading and wishing I could grow some fungi I finally got the chance to and so here I go!
I got my spores from spores101 (GT) and they arrived in 3 business days. GREAT! I got KER 1/2 pint jars (the tall ones, could not find short ones). Red Mill organic BRF and some fine vermiculite. I went to walmart and picked up an 8qt presto PC for around 40 dollars.
I began by mixing the ingredients: 1c BRF 1c water 2c verm I mixed them well and tried to break all the clumps that formed from the BRF.. after I mixed well I filled them about 3/4 inch form the top and filled that 3/4 inch with dry verm, and put the pre-drilled top on (4 holes for inoculation) then wrapped the tops with foil. I PCed 6 jars for 90 minutes(jars were not touching the bottom, had tops from other jars and foil on top like RR vid). I let the PC cool overnight (8 hours or so) and then inoculated. I wiped the needle with an alcohol pad after every hole and flamed the needle between jars. After that I put the tin foil back on and set them in my closet for colonization.
It has been basically 4 days and the jars are showing NO sign of colonization.. I injected each jar with about 2ccs of spore solution.. (used 10ccs for 6 jars) I figured it may be the temp because my house stays pretty dang cold most of the time.. sometimes even 60's.. I made an incubator by using 2 rubbermaids and a submersible water heater.. I mixed a solution of water/iso alcohol at about a 1:5 ratio. The heater is set at 78+2F so I'm guessing that the INSIDE of the jars is always at AT LEAST 80f.. I also bought a small heater that turns itself on and off and its adjustable, i set the temp at 80f in my room and it stays like that all day.. (I have 4 snakes so i think they appreciate it too!!)
Now for my newbie questions if anyone would be willing to answer: - Should I keep the incubator? There is obviously more chance for contams with it, but I wouldn't mind cleaning it and changing the water often (every 2 days or so). - 4 days in and no colonization AT ALL in ANY of the jars, should I be worried? - When I look at the spore syringe I see spores at the bottom, could it be that I didn't shake well enough so I didnt inject enough spores in the jars?
thank you all and happy to be part of the family!
-------------------- Whole Brown Rice Cakes TEK
"The mushrooms are just loving you back." "Maria Sabina is a symbol of wisdom and love."
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sickofit
*statement*


Registered: 12/01/10
Posts: 348
Loc: Local Park
Last seen: 1 year, 5 months
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Re: 1st BRF PF Golden Teacher Grow [Re: Swwert]
#14261274 - 04/08/11 11:31 PM (12 years, 10 months ago) |
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- Just keep it at 70 to 80 and no higher. mycelium progress declines and contam chances go up, or you can keep it at room temp but that takes longer to colonize. - Chill out, I promise in the future, there will be a time you'll forget there even there - Spores are very dense organic matter, they like to sit, you dont even need to shake them, moving it to your area and squirting some out is enough, think MILLIONS of spores.
Welcome, I was in your shoes not to long ago.
-------------------- A Resource on Psi, Science, and Philosophy The Taboo of Psi Trade/Want List Mechanisms and reactions Is ____ down, or is it just me? You know, its against the rules to discuss/trade research chemical sources on the board, oh hey look,a "contact user" button, isn't this site the greatest! Done(In order): Nicotine, THC, Psilocybin (Cubes,P. Cyans,Pan. Cinctulus), MDMA, LSD, 2C-E, Methampetamine, Lost track of how many JWH's, MDA, Nitrous Oxide, 2C-I, Cocaine, Ketamine, TMA-6, 2C-B, DOC, DXM, 4-MMC, MDAI, MDAT, MDPV, butylone, pentedrone, MXE, 25i, 25c, 25b, hydromorphone, 2 and 4-FA, 2 and 4 FMA, 6-apb, DMT
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Swwert
Truck-Boat-Truck


Registered: 04/08/11
Posts: 1,182
Last seen: 2 years, 10 months
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Re: 1st BRF PF Golden Teacher Grow [Re: sickofit]
#14261303 - 04/08/11 11:38 PM (12 years, 10 months ago) |
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Thanks for the quick reply! I'm just worried because none of the jars look like they have started.. but then again its probably a good thing.. maybe they are all going at the same pace. I shall be patient
-------------------- Whole Brown Rice Cakes TEK
"The mushrooms are just loving you back." "Maria Sabina is a symbol of wisdom and love."
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velvetmagician
Brewer



Registered: 03/01/11 
Posts: 422
Loc: Somewhere
Last seen: 11 years, 20 days
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Re: 1st BRF PF Golden Teacher Grow [Re: sickofit]
#14261325 - 04/08/11 11:44 PM (12 years, 10 months ago) |
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You sound exactly like what I did! Everything even using spores101!!! I also used tall 1/2 pint jars however I made the verm barrier a little thicker. I used 2 cc's per jar. I did not see growth for 10 days! Look at your jars, do you notice anyhing that looks like a grain of rice or a crystal meth looking shard?? Especially in your inoculation trail, the path the needle took. That, in my experience, is germination occuring. Be patient!!!!!! Temp should be 70-75 degrees. If you have tape over the holes, take em off. The verm barrier is your filter and Gas Exchange is needed to get your mycelium growing at a good rate. I use 4oz jars because they colonize so fast.I hear alot of folks saying tall 1/2 pint jars stall. I just upped the cc's and I didnt have stalled jars!!!! If you dont see growth in 10 days after noculation, change your location of colonizing jars. I had a buddy who we noced up with b plus. He had em in the closet. 11 days passed no signs of growth. I suggested to move one jar to another spot under his desk, and that jar took off the next day!!! Moved all jars to that spot and they are shredding!!!!! Hope this helps, have fun and be patient!!!!!!
-------------------- Q: How many Micheal Jackson's does it take to screw in a light bulb? A: NONE, he screws kids!
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Ajaxx
Amateur Mycologist



Registered: 03/06/11
Posts: 1,303
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Last seen: 12 years, 3 months
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i set my incubator to 84 degrees. its usually 82-84 degrees. inside the jars its going to be 1-2 degrees warmer because mycelium gives off heat and the glass keeps it in.
take a look at this picture taking from "the mushroom cultivator" by paul stamets and JS chilton
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Swwert
Truck-Boat-Truck


Registered: 04/08/11
Posts: 1,182
Last seen: 2 years, 10 months
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Thank you man, I will def try 4oz next time.. I think I'm being over-protective with the temp.. I have my incubator on and PLUS I have a small heater in the room on at all times keeping it at 80-82. But after reading Ajaxx's post I think I should keep it on?
-------------------- Whole Brown Rice Cakes TEK
"The mushrooms are just loving you back." "Maria Sabina is a symbol of wisdom and love."
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: 1st BRF PF Golden Teacher Grow [Re: Ajaxx]
#14261354 - 04/08/11 11:53 PM (12 years, 10 months ago) |
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you have to keep in mind when you're raising the temps that high you're giving mold spores the temps that they thrive in,
instead of giving mycelium the head start you're giving trich and bacteria the head start..
i would not go above 80
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Ajaxx
Amateur Mycologist



Registered: 03/06/11
Posts: 1,303
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Re: 1st BRF PF Golden Teacher Grow [Re: Swwert]
#14261361 - 04/08/11 11:55 PM (12 years, 10 months ago) |
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get an aquarium heater that you can adjust the temp on. get 2 of the exact same tubs. put the heater in the bottom of one tub and pour a bunch of water in it. set the heater to 82-84 degrees.
put your tub with all your jars into the tub with water and the heater. itll take a while for the heater to get the water warm, but itll keep your jars at the perfect temp.
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Ajaxx
Amateur Mycologist



Registered: 03/06/11
Posts: 1,303
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Last seen: 12 years, 3 months
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Re: 1st BRF PF Golden Teacher Grow [Re: biologys]
#14261366 - 04/08/11 11:56 PM (12 years, 10 months ago) |
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Quote:
biologys said: you have to keep in mind when you're raising the temps that high you're giving mold spores the temps that they thrive in,
instead of giving mycelium the head start you're giving trich and bacteria the head start..
i would not go above 80
as long as you sterilized everything properly you shouldnt have to worry. im sittin on 50 quart jars that have been incubating at 83-84 degrees and all 50 are contaminant free.
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: 1st BRF PF Golden Teacher Grow [Re: Ajaxx]
#14261413 - 04/09/11 12:10 AM (12 years, 10 months ago) |
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thats good for you, but just because you have one successful attempt doesn't mean that every single person who recommends not going over 80 are wrong....
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Re: 1st BRF PF Golden Teacher Grow [Re: Ajaxx]
#14261623 - 04/09/11 01:45 AM (12 years, 10 months ago) |
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Quote:
Ajaxx said: i set my incubator to 84 degrees. its usually 82-84 degrees. inside the jars its going to be 1-2 degrees warmer because mycelium gives off heat and the glass keeps it in.
take a look at this picture taking from "the mushroom cultivator" by paul stamets and JS chilton

Do I see 1958 mentioned in that graph? thats outdated bruv! I let my jars sit in 50-60 degree temps, they colonize just fine and even have LC jars that colonize within 1 week at those temps. You dont need an incubator for anything
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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DynGBreeD
┌∩┐(◣_◢)┌∩┐


Registered: 01/15/11
Posts: 3,639
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Re: 1st BRF PF Golden Teacher Grow [Re: Swwert]
#14261967 - 04/09/11 06:35 AM (12 years, 10 months ago) |
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Quote:
Swwert said: Thank you man, I will def try 4oz next time.. I think I'm being over-protective with the temp.. I have my incubator on and PLUS I have a small heater in the room on at all times keeping it at 80-82. But after reading Ajaxx's post I think I should keep it on?
Here is one of my 4oz. cakes.... Isnt he so cute! hahaha
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munchiegreen
King Kong



Registered: 02/23/11
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Re: 1st BRF PF Golden Teacher Grow [Re: DynGBreeD]
#14262085 - 04/09/11 07:55 AM (12 years, 10 months ago) |
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Don't use an incubator! Your temps seem fine without it
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Swwert
Truck-Boat-Truck


Registered: 04/08/11
Posts: 1,182
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Re: 1st BRF PF Golden Teacher Grow [Re: munchiegreen]
#14262190 - 04/09/11 08:40 AM (12 years, 10 months ago) |
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thank you all, beginning of day 4 and still nothing. I know patience is key but . I'll try to get pics when i get some action going
-------------------- Whole Brown Rice Cakes TEK
"The mushrooms are just loving you back." "Maria Sabina is a symbol of wisdom and love."
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Ajaxx
Amateur Mycologist



Registered: 03/06/11
Posts: 1,303
Loc:
Last seen: 12 years, 3 months
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Re: 1st BRF PF Golden Teacher Grow [Re: biologys]
#14262320 - 04/09/11 09:40 AM (12 years, 10 months ago) |
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i use an incubator because my room temp changes so damn much. i like to have total control over the environment while colonizing jars. ive tried colonizing at room temp and its never worked for me 
to each his own. whatever works.
Quote:
biologys said: thats good for you, but just because you have one successful attempt doesn't mean that every single person who recommends not going over 80 are wrong....
very true. sorry. im still a noob.
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lostinbq
Clap...or we will find u!!!!


Registered: 03/25/11
Posts: 525
Loc: ZombieLand
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Re: 1st BRF PF Golden Teacher Grow [Re: Ajaxx]
#14262392 - 04/09/11 10:03 AM (12 years, 10 months ago) |
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when you inoculate the jar with spore sol you then need to wait for the spores to germinate first. think of it as a seed that you put into the dirt, it needs a week to just poke its lil head above. well thats the way i look at it. thats why i made 2 lc's to kill that(up to a week)germination time of the spores. i just made my first jar last friday and another 2 on that following monday(pf tek style and i can only steam 2 at a time)the jar that is 1 week old today did not show anything till the end of the 4th day and it was like they said a small piece of meth looking junk on the brf, and now today i have a half dollar sized piece of myc growing in that spot that is breaking off into other pieces of growth. so if it is not noticeable today maby you will see it in tomorrow morning.
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Swwert
Truck-Boat-Truck


Registered: 04/08/11
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Re: 1st BRF PF Golden Teacher Grow [Re: lostinbq]
#14264752 - 04/09/11 10:40 PM (12 years, 10 months ago) |
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thanks all, still nothing.. at all. Great way to look at it lostinbq... it gives me a boost of hope!
-------------------- Whole Brown Rice Cakes TEK
"The mushrooms are just loving you back." "Maria Sabina is a symbol of wisdom and love."
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velvetmagician
Brewer



Registered: 03/01/11 
Posts: 422
Loc: Somewhere
Last seen: 11 years, 20 days
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Re: 1st BRF PF Golden Teacher Grow [Re: Swwert]
#14266665 - 04/10/11 11:39 AM (12 years, 10 months ago) |
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I would do away with incubator. This is out dated information. 72-75 is optimum!
-------------------- Q: How many Micheal Jackson's does it take to screw in a light bulb? A: NONE, he screws kids!
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Fryer Mike
Lost in the fourth dimension.


Registered: 02/22/09
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Re: 1st BRF PF Golden Teacher Grow [Re: DynGBreeD]
#14266826 - 04/10/11 12:21 PM (12 years, 10 months ago) |
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Quote:
DynGBreeD said: Here is one of my 4oz. cakes.... Isnt he so cute! hahaha
Would you say it's better to leave a pile of verm on top of the cake like in your pic? The verm I have doesn't seem to want to stick to the cakes.
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DON'T READ THIS! Any information/ pictures/ text that implies I grow or use illegal substances is false and/ or for entertainment purposes. Nothing I say or submit to this forum or to any other should be taken literally. I am become death, destroyer of worlds. Fryer Mike
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velvetmagician
Brewer



Registered: 03/01/11 
Posts: 422
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Re: 1st BRF PF Golden Teacher Grow [Re: Fryer Mike]
#14267137 - 04/10/11 01:33 PM (12 years, 10 months ago) |
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Did you dunk the cakes for 24 hours? Also, when you rolled the dry verm, you should wait 30 to 45 minutes before misting so the verm sticks to the cake better. If you roll then mist right away the verm will get washed off, not stick to the cake good
-------------------- Q: How many Micheal Jackson's does it take to screw in a light bulb? A: NONE, he screws kids!
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