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Invisiblewildernessjunkie
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Age or P-Value of cultures?
    #14260194 - 04/08/11 06:46 PM (12 years, 9 months ago)

After reading hamloaf's thread on understanding senescence, I have a question regarding the Age or P-Value of cultures. At what point would you consider a culture "New" or "Young"? At what point would it be considered "Old" and possibly ready to be discarded? I understand the basics behind each way of measuring, and understand the idea behind both. Where are the limits?


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OfflineRogerRabbitM
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Re: Age or P-Value of cultures? [Re: wildernessjunkie]
    #14260396 - 04/08/11 07:48 PM (12 years, 9 months ago)

It has to be your own system.  One guy might let a P1 petri dish grow fully out before taking a sample to P2.  Another grower might only let the mycelium go 1/4" before grabbing a culture to P2.  Both would have the same P value but one would be ten times the number of cell divisions as the other.  That system only works for your own cultures where you know exactly how long you allow each transfer to grow, before inoculating master slants.
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Invisiblewildernessjunkie
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Re: Age or P-Value of cultures? [Re: RogerRabbit]
    #14260600 - 04/08/11 08:39 PM (12 years, 9 months ago)

So overall its still a very vague system then? I was reading in the other thread about how you measured the age of your master culture tubes in days. Were those measured in days without refrigeration only, and hence allowing growth? What temp would cultures need to be stored in order to inhibit the growth? Will a standard refrigerator suffice?


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OfflineRogerRabbitM
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Re: Age or P-Value of cultures? [Re: wildernessjunkie]
    #14261945 - 04/09/11 06:13 AM (12 years, 9 months ago)

Many people have lost cultures putting them in a normal refrigerator.  They don't maintain a steady temp, and the fluctuations cause condensation which eventually ruins the culture.

If you have to use a non-lab refrigerator, be sure to keep the slants inside an insulated lunch box or similar to buffer the temp swings of the fridge.

My slants keep very young cell lines, because I never warm them up before using.  I take them out of refrigeration to make the transfer and then put them right back.  As soon as I have clean growth, more slants are made, usually with no more than four or five days of cell divisions before the new slants are back in refrigeration.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblewildernessjunkie
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Re: Age or P-Value of cultures? [Re: RogerRabbit]
    #14262942 - 04/09/11 12:32 PM (12 years, 9 months ago)

Perfect. I think Ive got what I need now. All I have is a standard refrigerator, so Ill go get a lunchbox and Ill be squared away. Thanks for your help.


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