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Invisiblecyanara
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do I need to wash my tubes before making my slants?
    #14227890 - 04/02/11 07:28 PM (12 years, 10 months ago)

jst wondering abot to do it right now.

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Invisiblejokefox
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Re: do I need to wash my tubes before making my slants? [Re: cyanara]
    #14229646 - 04/03/11 02:00 AM (12 years, 10 months ago)

your tubes need to be sterilized,

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Invisiblecc2
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Re: do I need to wash my tubes before making my slants? [Re: jokefox]
    #14229890 - 04/03/11 04:22 AM (12 years, 10 months ago)

depends how were your tubes stored and what did they contain.
if they were clean and dry to the eye you could omit sterilizing them, AFAIK there are still no micro organisms which inhabit unnutritive glass :smile:

however better safe than sorry, I'd hate to get contams just 'cos I was too lazy to boil 'em :thumbdown:

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Offlinesolumvita
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Re: do I need to wash my tubes before making my slants? [Re: cc2]
    #14230841 - 04/03/11 11:49 AM (12 years, 10 months ago)

Yes you need to clean your tubes, if you are pouring the agar into the tubes, then the tubes should be sterilized as well.  If you are making the agar in the tubes and are going to allow the tubes to solidify at an angle then you should be fine.


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InvisibleBacchus
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Re: do I need to wash my tubes before making my slants? [Re: solumvita]
    #14231334 - 04/03/11 02:13 PM (12 years, 10 months ago)

Yeah. Why wouldn't you? I wash all of my tubes with a test tube brush using soap and water. I then rinse very thoroughly and pour the (extremely well mixed) agar solution into each tube. Screw the caps down tight and sterilize.


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Living on a no-Flash diet is way easier than you think. Give it a shot.

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Offlineshroomie_glen
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Re: do I need to wash my tubes before making my slants? [Re: Bacchus]
    #14259927 - 04/08/11 05:46 PM (12 years, 10 months ago)

When in doubt, sterilize.


--------------------

No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.

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Invisiblecyanara
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Re: do I need to wash my tubes before making my slants? [Re: shroomie_glen]
    #14260271 - 04/08/11 07:14 PM (12 years, 10 months ago)

:laugh2:

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InvisibleD.C
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Re: do I need to wash my tubes before making my slants? [Re: Bacchus]
    #14288407 - 04/14/11 03:57 AM (12 years, 10 months ago)

Quote:

Bacchus said:
Why wouldn't you?




What he said. A thousand times over, what he said. Why would you even bother getting on the forums to make a post where you essentially ask for someone to give you permission to be lazy? By the time you make the post and read the replies, you could have already washed several tubes.

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Invisiblecyanara
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Re: do I need to wash my tubes before making my slants? [Re: D.C]
    #14289110 - 04/14/11 09:52 AM (12 years, 10 months ago)

was there a point here?

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