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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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Offlinequebus
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Registered: 05/01/10
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LC to Agar time
    #14259382 - 04/08/11 04:01 PM (12 years, 9 months ago)

Recently inoc'd agar (grocery tek) with LC and it's been four days.  I'm not sure what the time-frame is.  Need to set my expectations. The agar might still have been cold from the fridge when I did the transfer while the LC was room temp.

So -how long before I should see myc?


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Offlinequebus
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Re: LC to Agar time [Re: quebus]
    #14260262 - 04/08/11 07:09 PM (12 years, 9 months ago)



Reply would be most appreciated


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InvisibleDoc_T
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Re: LC to Agar time [Re: quebus]
    #14261333 - 04/08/11 11:48 PM (12 years, 9 months ago)

Should happen pretty soon if it's going to happen, I would guess.

I note that you didn't give us even a tiny hint of information- no temps, no recipe, no indication of the size or status of teh agar wedge.
Other than it might have been cold, I mean.

So asking for an answer is a bit improper. Ask a better question and you get better answers.


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Offlinequebus
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Re: LC to Agar time [Re: Doc_T]
    #14261933 - 04/09/11 05:56 AM (12 years, 9 months ago)

Thanks Doc.  Although - I mentioned grocery agar tek.  I referenced that the agar was right out of the fridge.  And there was no wedge - just LC to agar.  Not sure what else to add except the LC was room temp - call it 70d F.

Thanks again.


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OfflineTerry M
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Re: LC to Agar time [Re: quebus]
    #14261944 - 04/09/11 06:09 AM (12 years, 9 months ago)

Question about LC inoculation of agar: Does the result always have a messy center of growth? Mine does, even when I use a single drop of LC and am careful to keep the plate horizontal and motionless overnight, until the liquid settles in to the agar.

I've gotten in the habit of making a second, much neater plate by cutting a square of the LC plate center.


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Liberté, égalité, humidité.


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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


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