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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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Rye problems
#14258222 - 04/08/11 12:01 PM (12 years, 9 months ago) |
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Not sure what the problem is here. Using my oyster agar jars I did an Agar to rye transfer in quart jars and was simply amazed. The next day the mycellium had jumped off and grown about the size of a golfball stretching for anything to eat up. A rate that looked like within a week it would be 100%. I waited a few days untill it had several spots looking nice and gave it a good shake. It's been over a week and some jars colonization is very slow, a couple of jars I don't see anything at all. Did I kill the myc by shaking too hard? wtf? I don't understand, is my rye not prepared correctly. Out of about 8 jars 0 contams all looked great. What's going on?
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Perun
Mahapralaya...



Registered: 11/11/10
Posts: 585
Last seen: 2 years, 2 months
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Rye is not prep correctly(moisture level) or u DO have a contam so myc cant recover!
-------------------- First: Then:
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Microppose
Things Maker



Registered: 11/30/10
Posts: 849
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Re: Rye problems [Re: Perun]
#14258277 - 04/08/11 12:15 PM (12 years, 9 months ago) |
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Perhaps not enough GE, polyfil too tightly packed, or the inverted jar lids are screwed on too tight. Either one of those issues or your grain is drying. I've noticed that if you shake after the mycelium has absorbed much of the water, it moves slower. Try loosening the jar lid if you are using them inverted. If you packed the polyfil too tight, I have no answers.
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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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Wtf is pollyfill? I used none of that. I soaked my grain overnight boiled for 10-12 minutes, loaded half full and pc'd for 45 mins.
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Perun
Mahapralaya...



Registered: 11/11/10
Posts: 585
Last seen: 2 years, 2 months
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Quote:
randy420rhoads said: Wtf is pollyfill? I used none of that. I soaked my grain overnight boiled for 10-12 minutes, loaded half full and pc'd for 45 mins.
There u go...contam! 45 min is not enough...90min and more is what u want!
If u shake and myc is not recovering,that is a sign of contam...usualy!
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
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could be what Perun has mentioned or your lacking GE. any pics of your lid setup?
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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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If it's necessary I can snap a few but they're just regular quart jars rubber side down. So GE is definately a possibility. And as far as the 45 mins I forgot 4 jars were at 45 the rest were 90. For some reason 2 batches ( I can only fit 2 jars a load) had all the water disappear right after 45 mins of pcing.
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Perun
Mahapralaya...



Registered: 11/11/10
Posts: 585
Last seen: 2 years, 2 months
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So u dont have any GE holes? What is the lid set up? Filter? water disappear? From where? PC? If u r reffering jars...u dont want water inside your jars...all the water u need is inside your rye!
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Rye problems [Re: Perun]
#14259641 - 04/08/11 04:51 PM (12 years, 9 months ago) |
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If you have sealed the jars and provided no filtered gas exchange that would definitely explain why the growth has slowed down and in some cases stopped.
Mycelium needs oxygen to grow and expand.
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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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No GE holes at all I know they help but didn't see anything about being mandatory. I couldn't get ahold of any tyvek to make a filter.
And yes the water in my PC disappeared right after the 45 minute mark so I had to turn it off. Guess I didn't add enough for the 2 batches. There is no water in my jars just nice fluffy rye.
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Microppose
Things Maker



Registered: 11/30/10
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As I mentioned earlier, you should have inverted your lids to create an imperfect seal that allows for GE but prevents mold spores from entering your jars. Rubber side down will essentially seal the jars from everything including precious air.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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You need to provide filtered gas exchange through tyvek, polyfil, or synthetic filter discs. That is issue #1.
#2 is the 45 minutes sterilization time.
Choose a method to provide filtered gas exchange to your jars and start again would be my opinion.
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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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Any suggestions on filters. Cheapest tyvek at home depot was a huge role for a couple hundred bucks. Went to 3 different post offices when I asked for tyvek envelops they looked at me funny and said they don't carry them. Do I really have to break down I pay a shitload in shipping for the premade filters?
Only 4 of the jars were pc'd at 45 mins so i'll check on any differences between.
I just can't seem to get the rye to work. I bought a pre sterilized rye grain bag from everything mushrooms with an injection port. Injected half a syringe into it it's been like a month and no signs of growth, or off odors. Simply nothing.
If there's a crack in my closet that lets a tiny ammount of light in is that ok or unacceptable?
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Microppose
Things Maker



Registered: 11/30/10
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If you are inoculating by GTG transfer, invert your lids. If you are inoculating by spore syringe, polyfil and tyvek filters are the easiest and most readily available improvised filter material. Look up teks on the search function.
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
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Quote:
randy420rhoads said: No GE holes at all I know they help but didn't see anything about being mandatory. I couldn't get ahold of any tyvek to make a filter.
I had no problems getting tyvek envelopes at the post office.
The pleasant gentleman asked how many I would like... I said 30 and the guy didn't even blink about it.
indirect light is at worst harmless during colonization and at best beneficial.
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healing
Strangest



Registered: 02/22/11
Posts: 6,565
Loc: the universe, the milky w...
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You know how Fedex and UPS have those little boxes they put outside of the post office and other places? Those have big stacks of tyvek envelopes in them.
-------------------- Open mind, open heart, open book.
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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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Re: Rye problems [Re: healing]
#14260109 - 04/08/11 06:28 PM (12 years, 9 months ago) |
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Quote:
Azurascender said: If you are inoculating by GTG transfer, invert your lids. If you are inoculating by spore syringe, polyfil and tyvek filters are the easiest and most readily available improvised filter material. Look up teks on the search function.
It was agar to grain.Quote:
healing said: You know how Fedex and UPS have those little boxes they put outside of the post office and other places? Those have big stacks of tyvek envelopes in them.
Lmao sounds like a federal offense.
Guess these post officer workers are just idiots. They had no idea what I was talking about. I serious explained it for like 10 minutes to each one.
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bakenast
Muffen Stuffer


Registered: 03/14/11
Posts: 920
Last seen: 1 year, 26 days
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u can get tyvek envelopes from usps.com for free with free shippin to u
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healing
Strangest



Registered: 02/22/11
Posts: 6,565
Loc: the universe, the milky w...
Last seen: 6 years, 6 months
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Quote:
randy420rhoads said:
Quote:
Azurascender said: If you are inoculating by GTG transfer, invert your lids. If you are inoculating by spore syringe, polyfil and tyvek filters are the easiest and most readily available improvised filter material. Look up teks on the search function.
It was agar to grain.Quote:
healing said: You know how Fedex and UPS have those little boxes they put outside of the post office and other places? Those have big stacks of tyvek envelopes in them.
Lmao sounds like a federal offense.
Guess these post officer workers are just idiots. They had no idea what I was talking about. I serious explained it for like 10 minutes to each one.
They're there for you to take.
-------------------- Open mind, open heart, open book.
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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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Re: Rye problems [Re: bakenast]
#14260245 - 04/08/11 07:02 PM (12 years, 9 months ago) |
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Edited by randy420rhoads (04/08/11 07:02 PM)
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quebus
Stranger

Registered: 05/01/10
Posts: 678
Last seen: 2 months, 23 days
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Re: Rye problems [Re: healing]
#14260253 - 04/08/11 07:06 PM (12 years, 9 months ago) |
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How did you check to see your water was gone after 45 minutes? Sumptin is fishy.
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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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Lol "misuse could be a violation of federal law, do you accept?"
duh.
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randy420rhoads


Registered: 02/24/07
Posts: 535
Last seen: 11 years, 5 months
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Re: Rye problems [Re: quebus]
#14260265 - 04/08/11 07:10 PM (12 years, 9 months ago) |
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Quote:
phortre55 said: How did you check to see your water was gone after 45 minutes? Sumptin is fishy.
After it stops rocking, without any steam, and you smell an off odor, I find it's a good idea to check it out sure enough. Bone dry. I fixed the problem for the other loads. More rings. More water.
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Microppose
Things Maker



Registered: 11/30/10
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Randy, Agar to grain is essentially the same game, transferring live tissue to a jar. As long as you don't have to inoculate via syringe, you don't REALLY need a filter of any kind other than the inverted lid method. Simplest and cheapest method, because there are no other purchases necessary...
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