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2jew4u
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Registered: 04/07/11
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Pan Cyan(Poo)
#14257487 - 04/08/11 08:47 AM (12 years, 9 months ago) |
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How many days does it usually take, for you to see signes of growth from inoculation? Have some that has ran for 7 days and it shows half dollar size growth, in each of the injection sites, But just wanted to make sure it wasnt cobweb mold.
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ninja cat 09
A paranoid android



Registered: 10/11/09
Posts: 4,170
Loc: Mexico
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14257624 - 04/08/11 09:34 AM (12 years, 9 months ago) |
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It can take from 1 to two weeks, occasionally more.
This question has been answered quite a few times before, the search engine in this site is a valuable tool. 
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2jew4u
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Yea i know thats why i was asking, seems a lil early, And the mighty search engine says that the mycelium kinda looks like cobweb, so thats y i was askin.
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potgrrl
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14259349 - 04/08/11 03:55 PM (12 years, 9 months ago) |
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If it's infection, you'll know within days of seeing that first sign.
Infection spreads like mad, mycelium - not so much.
With healthy fresh spores it's possible to see some growth within 7 days for sure, but it'll still take 2-3wks to colonize
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esquaredx



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Re: Pan Cyan(Poo) [Re: potgrrl]
#14259648 - 04/08/11 04:52 PM (12 years, 9 months ago) |
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Pan mycelium is more wispy. It does give off the look at first of cobweb.
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ManicMongrel
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Registered: 03/14/11
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I'm growing pan cyan myself at the moment, just want to add that they are very temperature sensitive in my experience.
I had two germinated jars at 24C, and could barely see a little mycelia after 4 days, then I raised the temp to 28C and the whole jar was colonized in a little over 2 days. Yup it can grow fast, so fast that it can be a pain to tell the difference from cobweb.
It also looks quite similar cobweb on the border of the colony, in the way it stretch out to colonize, then it fills up from the center and outwards with a very cottony texture.
And yes, I have the actual fruits to know its pan cyan. I don't have any pictures to prove it though, so can't help if its hard to believe :P
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2jew4u
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So u raised it to 82f and they colonized in 2 days later Wow- Did you do them Pf style, with Poo added? or how did you do it- Bc from what it seems they colonize way faster then cubes
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ManicMongrel
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14263254 - 04/09/11 02:18 PM (12 years, 9 months ago) |
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I can't help myself from preaching about hay :P So here it comes:
I took hay thats usually used as food supplement for cattle, whats good about this is that its stored with ammonia for many months. Which means the crystalline fence of celluloses and lignine is dissolved somewhat so that nutrients are more easily extracted when the cow digests it.
Since the fungus is a primary decomposer, the hay is now very easily digested.
I cut the hay in inch size pieces, boiled it in two turns 12h apart to get rid of the ammonia and two kill competing fungi. What I got was a dark brown mix with a fair bit of nutrients solved in the excess water.
I used it for both spawning and bulk substrate and its been awesome for both. It has very little carbohydrates like sugar in it, no protein which might explain why I haven't had ANY contamination problems so far! Its even exposed to mold spores from the air every now and then, when I add some coffee grounds to raise levels of phosphate, nitrogen and minerals.
I made crude spore vials from cap pieces shaken in a sterile bottle with drinking water from 7-eleven in Thailand. I don't know where it gets the nitrogen from, could be from the ammonia or else I probably got some nitrogen fixing bacterias with it, considering it turned out to be contaminated as hell, the hay was my last attempt with the last of my spore mix.
Edit: This it probably basic stuff for most people, but I forgot to add that hay is almost all celluloses, its very poor for other nutrition, in case some of you didn't knew. Its why grass can be a mold nightmare as soon as it gets wet, since its pretty nutritious overall. While hay usually stays the same.
-------------------- Rules and laws are guidelines, to follow them by definition is equivalent to ignoring responsibility - Let me know if anything in my Trade List got your attention! New asian pan varieties up for trade [10.july.2013] "Familiarity breeds contempt"
Edited by ManicMongrel (04/09/11 02:32 PM)
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ManicMongrel
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14263465 - 04/09/11 03:23 PM (12 years, 9 months ago) |
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Yup, I raised the temperature to 82F and the growth speed took a quantum leap. From the FAQ in the archive they are supposed to grow very fast. I haven't grown cubensis yet, so I cant really compare them.
-------------------- Rules and laws are guidelines, to follow them by definition is equivalent to ignoring responsibility - Let me know if anything in my Trade List got your attention! New asian pan varieties up for trade [10.july.2013] "Familiarity breeds contempt"
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esquaredx



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What are you using for this grow? Post some pics
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ManicMongrel
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I'm using hay from barley and oat (you know, whats left of the straw after they cut of the grains)
For the spawning I use this only, for the bulk substrate I use a mix of hay, coffee grounds and horse dung.
Here is few pics showing one of my spawning jars:



This jar is a little old (2-3 weeks), which is why it has pins, I removed 3 shrooms already. It's kept below its ideal temp, so it grow a lot slower, I just use it as a backup culture in case the bulk substrate goes to hell. A few pieces of this in a new jar, and its colonized in under a week, it doesn't colonize as fast as spores though but it works.
The culture in my bulk is working on coffee grounds i added a few days ago, so I don't want to open it and mess with the moisture levels until all of it is fully colonized, then I'll take a few pics.
As a next experiment I'm going to mix fresh-from-the-factory-horse-poop with wheat bran, let it brew for a while before I pasteurize and use it for bulk substrate. Hopefully the fermentation will remove all of the starch and sugar, anyway I'll let you pan growers know how it turn out.
-------------------- Rules and laws are guidelines, to follow them by definition is equivalent to ignoring responsibility - Let me know if anything in my Trade List got your attention! New asian pan varieties up for trade [10.july.2013] "Familiarity breeds contempt"
Edited by ManicMongrel (04/10/11 05:48 AM)
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2jew4u
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Mainic- u were semi right( well at least for me) About raising the temps, raised them to 80 and wow did they take off- wonder why they are so much faster than cubes?, They (shea tek pans without a pc,but i pced them) are almost all the way colonized on the out side of the jar, and were knocked up on the 5th, they are prolly about 60-70 pct now. Just hope its not cobweb, but the mass does look white the edges just look kinda gray.
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ManicMongrel
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14266515 - 04/10/11 11:05 AM (12 years, 9 months ago) |
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I don't think you have cobweb, just keep an eye on the texture of it. Cobweb looks a more like long haired fur and keep the same texture, while Pan. change into resembling very thin-fibered cotton with a few thicker hairs here and there (transport tubes between mated colonies?).
Pan. also starts building different formations so dont get too hysterical about contams, only contams I would look out for is green mold, but thats not very likely with dung.
By the way, Pan. tend to pick up different pigments in the substrate so don't worry if it's not pure white everywhere as long as the texture of fibers seem right.
I was told that ideal temp is very strain dependent in a microbiology class once, probably why yours was a few deg different.
Good luck!
-------------------- Rules and laws are guidelines, to follow them by definition is equivalent to ignoring responsibility - Let me know if anything in my Trade List got your attention! New asian pan varieties up for trade [10.july.2013] "Familiarity breeds contempt"
Edited by ManicMongrel (04/10/11 11:13 AM)
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2jew4u
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Thanks, Hope it works, at least be able to get a fruit to clone and get more spores. And i can see the difference depending on the strain- the one that does best on your sub/mix, temps- is going to work for you- since everybody climate and subs may very. makes perfect sense-
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2jew4u
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14267698 - 04/10/11 03:54 PM (12 years, 9 months ago) |
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Also do you think that they prefer light when colonizing?
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ManicMongrel
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14270324 - 04/11/11 12:43 AM (12 years, 9 months ago) |
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I wouldn't keep them in a completely dark environment. Mine gets dim daylight from my window, and it all works well. I'm pretty sure they prefer day/night cycles of light, the pinning seem to happen at night time and its also when the fruits grow quickest.
By the way, you can just take a clean piece of mycelium from a culture and spawn directly to a fresh fluffy mix of horse poop if you get infections. I have even done the same with coffee grounds, I wouldn't be surprised if you can clone on it too
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2jew4u
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Kewl thanks for the info. keep u updated
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ManicMongrel
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14273096 - 04/11/11 03:46 PM (12 years, 9 months ago) |
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Ok, this is kind of off topic... I picked three mushrooms from my bulk today, threw it in mixer along with the weak ones from the spawn jar. It actually made a decent trip!
They didn't bruise blue either. I have read arguments that the blue color is not actually psilocybin getting oxidized but, rather a protein used in the synthesis of psilocybin/psilocin. So potent shrooms might bruce deep blue if they use one version of the protein, since they have lots of it, or not depending on which version of the gene they have activated. Anyway, point is apparently Pans don't have to bruce blue to have effect... Kind of cool...
-------------------- Rules and laws are guidelines, to follow them by definition is equivalent to ignoring responsibility - Let me know if anything in my Trade List got your attention! New asian pan varieties up for trade [10.july.2013] "Familiarity breeds contempt"
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2jew4u
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They stalled, for some reason. still like a half dollar to quarter size that didnt colonize, doesnt smell bad or any thing, so dunno
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mister
Nature nut



Registered: 10/04/08
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14300214 - 04/16/11 09:52 AM (12 years, 9 months ago) |
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You should of really found some cow manure and mixed that in with brf cakes at 1/3 ratio each verm, poo, brf. That would of been a good start for pans. Then just fruited them like a PF style jar without removing the sub from the jar, just keep the top verm layer moist and they will start poping up like cubes. but this is what has worked for me, the black kow manure to be exact, but smells horrible when sterilizing. They are very picky about what they will fruit from. It took me several tries before I found that out. But it was a fun time experimenting with them when I was.
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   AMU Q & A thread  
Edited by mister (04/16/11 09:58 AM)
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ManicMongrel
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Re: Pan Cyan(Poo) [Re: 2jew4u]
#14300499 - 04/16/11 11:41 AM (12 years, 9 months ago) |
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Maybe the mycelium is starved on oxygen, it happens when I'm too sloppy, fluffy mixes always grows best for me. If you think that might be the problem I would try to make more ventilation on one of them, see if anything happens.
If you really want to play safe, you could also transplant some mycelia to a mix with poo(3/4) and coffee grounds (1/4) and make more jars, it shouldn't take long before you have plenty of jars to fruit from. I'm not sure about verm content, I'm not into it since I have plenty of straw available :-P
It shouldn't take long before everything is up and running again :-)
-------------------- Rules and laws are guidelines, to follow them by definition is equivalent to ignoring responsibility - Let me know if anything in my Trade List got your attention! New asian pan varieties up for trade [10.july.2013] "Familiarity breeds contempt"
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