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OfflineRyan45867
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Registered: 04/07/11
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Last seen: 12 years, 8 months
Inoculation questions
    #14255197 - 04/07/11 07:45 PM (12 years, 9 months ago)

I am going to inoculate the jars tonight but just have one question.  There are 2 layers of foil over the jars I have.  Do I lift the first layer and inject the spore solution through the second on, then place the first layer back on top?  Basically, do I have to cover up the holes I make with the syringe after-wards?

Also, does the humidity level inside the growing chamber matter during this part?

Thanks!


Edited by Ryan45867 (04/07/11 07:50 PM)


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InvisibleSporesAndSpores
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Registered: 01/19/11
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Re: Inoculation questions [Re: Ryan45867]
    #14255228 - 04/07/11 07:50 PM (12 years, 9 months ago)

I think you should do a little more reading on here. You seem a little confused, the jars do need gas exchange for the mycelium to grow so holes need to be open. If your doing Pf style jars the dry verm on top acts as a filter.  But if not you need a filter for those holes.  Also jars can incubate at room temperature in a dark droor or anywhere else with no light, the fruiting chamber is for when you take the cakes out of the jars and they produce mushrooms, so humidity isnt a factor during the colonization period.  Watch this video and don't worry about micropore tape, I use clear tape so i can see the holes when i inoculate and then i take it off.  I assume your doing the Pf Tek correct?


Edited by SporesAndSpores (04/07/11 07:54 PM)


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InvisibleBacchus
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Re: Inoculation questions [Re: Ryan45867]
    #14255249 - 04/07/11 07:54 PM (12 years, 9 months ago)

Are you using wide mouth canning jars with four holes punched in each lid? If so, take off the foil as soon as your jars are cool enough to touch. Also, take off any tape that you may have over the holes. The dry layer of vermiculite is the only protection that you need. If you're using a drinking glass with foil as your lid, then you probably want to leave the foil on :tongue:

The jars don't need to be stored in the fruiting chamber during colonization. All of the humidity and carbon dioxide are inside the jar itself. I let my jars colonize in a rarely used kitchen cupboard. Sock drawer, bookshelf, closet, under the bed... Doesn't really matter where you keep them (as long as they don't suffocate... Inside a plastic bag would be bad.)


--------------------


Living on a no-Flash diet is way easier than you think. Give it a shot.


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