Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Grow Bags   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineaquilon
Stranger

Registered: 04/07/11
Posts: 12
Last seen: 12 years, 9 months
LC First-Timer, Procedure Rough-Draft
    #14252918 - 04/07/11 12:43 PM (12 years, 9 months ago)

hey everyone,
  I've been reading here for a long time, I've grown plenty of cakes, and cased a few quarts of rye spawn. I'm going to move up to LC + bags, but I'll probably need plenty of pointers and insight/foresight from the experts. here is my vision:


1. Prepare Broth.
  a. find a broth recipe from a TC & brew it
  b. half-fill narrow-mouth quarts
  c. tyvek lids w/ RTV injection ports (or should I skip RTV & just tape over the hole?)
  d. PC

2. Inoculate LC
  a. in a still air box of course

3. Incubate LC
  b. 83 deg F?

4. Blend LC
  a. PC blender blade & seal
  b. combine 2 (half-full) jars at a time (is this a good way to hybridize strains?)
  c. affix blender blade & seal in place of narrow-mouth jar lid
  d. blend it

5.  Draw-Off Inoculation of Grain Bags
  a. order & receive many 1Lb rye bags from a sponsor
  b. pump LC into grain bags with the Allflex 20VM Draw-Off Syringe
  c. what gauge needle should I use for the blended LC? how many mLs LC/Lb rye?

6. Incubate the bags or not?

7. Time this whole operation to make outdoor beds w/ pasteurized coir in July and August

  can't wait to hear back from this community, please correct my language if you think it is improper


Extras: Filter Print Post Top
Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: LC First-Timer, Procedure Rough-Draft [Re: aquilon]
    #14252958 - 04/07/11 12:51 PM (12 years, 9 months ago)

Look up in the bottom of my post, you'll see "Agars Grain LC Tek", click on that, it'll save ya alot of trouble, as well you'll know your culture is clean, instead of using spores to nocc up a LC jar..


incubation is not needed, as long as its not super cold, like under 65...but dont go over 80


Extras: Filter Print Post Top
Offlinemrtoad25
groovy
 User Gallery

Registered: 02/28/11
Posts: 98
Last seen: 1 year, 11 months
Re: LC First-Timer, Procedure Rough-Draft [Re: biologys]
    #14253070 - 04/07/11 01:11 PM (12 years, 9 months ago)

I am doing a Lc with warm weather buttons. I siliconed the hole in the top for my needle. When I boiled the honey water it became air tight. So when i stuck my spore syringe in through the silicone it sucked all 12 cc out of my syringe. I was not happy about that.... I still have it air tight and it has been 2 weeks and the mycelium is growing very slow but I don't think I have to worry about it being contaminatted.


Extras: Filter Print Post Top
Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14253082 - 04/07/11 01:15 PM (12 years, 9 months ago)

LC is all about having nice lids with good injection ports and filters and personally I really like the rubber lid gaskets that fit under the plastic lids.

The better your lids the more success u will have.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


Edited by anonjon (04/07/11 01:16 PM)


Extras: Filter Print Post Top
Offlinemrtoad25
groovy
 User Gallery

Registered: 02/28/11
Posts: 98
Last seen: 1 year, 11 months
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14253096 - 04/07/11 01:18 PM (12 years, 9 months ago)

Well with out having any air or gas exchange it will prevent caontams. Is that correct? Also it will just take a long time to grow the mycelium?


Extras: Filter Print Post Top
Offlinehappihourhero
Waitin..
Male

Registered: 02/18/11
Posts: 115
Last seen: 11 years, 7 months
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14253109 - 04/07/11 01:19 PM (12 years, 9 months ago)

Quote:

mrtoad25 said:
I am doing a Lc with warm weather buttons. I siliconed the hole in the top for my needle. When I boiled the honey water it became air tight. So when i stuck my spore syringe in through the silicone it sucked all 12 cc out of my syringe. I was not happy about that.... I still have it air tight and it has been 2 weeks and the mycelium is growing very slow but I don't think I have to worry about it being contaminatted.





It shouldnt be air tight like that with the right lid.


Thats what my LC's will be in.


Extras: Filter Print Post Top
Offlinemrtoad25
groovy
 User Gallery

Registered: 02/28/11
Posts: 98
Last seen: 1 year, 11 months
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14253119 - 04/07/11 01:21 PM (12 years, 9 months ago)

Happihour
Right but your's is not air tight you are still allowing gas echange with the polly fill


Extras: Filter Print Post Top
Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14253122 - 04/07/11 01:22 PM (12 years, 9 months ago)

u don't want it air tight


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


Extras: Filter Print Post Top
Offlinemrtoad25
groovy
 User Gallery

Registered: 02/28/11
Posts: 98
Last seen: 1 year, 11 months
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14253130 - 04/07/11 01:23 PM (12 years, 9 months ago)

What is wrong with having it air tight. Doesn't it just slow down growth speed?


Extras: Filter Print Post Top
Offlinehappihourhero
Waitin..
Male

Registered: 02/18/11
Posts: 115
Last seen: 11 years, 7 months
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14253156 - 04/07/11 01:28 PM (12 years, 9 months ago)

GE is key. I want my LC's done asap so i can start on my 4 64qt mono's. :-D


Extras: Filter Print Post Top
Offlinemrtoad25
groovy
 User Gallery

Registered: 02/28/11
Posts: 98
Last seen: 1 year, 11 months
Re: LC First-Timer, Procedure Rough-Draft [Re: happihourhero]
    #14253178 - 04/07/11 01:33 PM (12 years, 9 months ago)

See i have time because i have B+ in the fruit chamber now. It would just make sense to me that less gas exchange means less chance of contams. Good gas exchange fast myc growth. If you think about it. When you Knock up a brf you cover it in foil so it really doesn't get gas exchange.


Extras: Filter Print Post Top
Offlinehappihourhero
Waitin..
Male

Registered: 02/18/11
Posts: 115
Last seen: 11 years, 7 months
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14253228 - 04/07/11 01:42 PM (12 years, 9 months ago)

Correction. The foil only stays on while you pressure cook. Once they leave the PC, the foil comes off. Cakes should have the dry verm layer to keep them safe.


Extras: Filter Print Post Top
Offlinemrtoad25
groovy
 User Gallery

Registered: 02/28/11
Posts: 98
Last seen: 1 year, 11 months
Re: LC First-Timer, Procedure Rough-Draft [Re: happihourhero]
    #14253242 - 04/07/11 01:44 PM (12 years, 9 months ago)

Well FMR I have been leaving the foil on. I will now have to remember to take it off after i sterilize the brf.


Extras: Filter Print Post Top
Offlineaquilon
Stranger

Registered: 04/07/11
Posts: 12
Last seen: 12 years, 9 months
Re: LC First-Timer, Procedure Rough-Draft [Re: aquilon]
    #14253294 - 04/07/11 01:55 PM (12 years, 9 months ago)

i think GE is important if you have a lot of broth to culture. breathable tyvek will keep out the contams.

  what temp to incubate cubensis LC?
  is my blending procedure legit?
  can a blended LC (like yeast/dextrose/agar) pass through an 18ga needle?
  how many mLs LC/Lb rye grain?

please stay on topic people, this isn't a thread about cakes or gas exchange


Edited by aquilon (04/07/11 01:59 PM)


Extras: Filter Print Post Top
Offlinehappihourhero
Waitin..
Male

Registered: 02/18/11
Posts: 115
Last seen: 11 years, 7 months
Re: LC First-Timer, Procedure Rough-Draft [Re: aquilon]
    #14253331 - 04/07/11 02:02 PM (12 years, 9 months ago)

I think the blending idea sounds awful. Opening your jar to pour them into the blender is going to allow contams in.

I have never heard of anyone doing such. And why would you want to try to cross strains? These are mushrooms, not marijuana. It's a FUNGUS, not a plant.

Stick with what works. You'll be much happier.


Extras: Filter Print Post Top
Offlinemrtoad25
groovy
 User Gallery

Registered: 02/28/11
Posts: 98
Last seen: 1 year, 11 months
Re: LC First-Timer, Procedure Rough-Draft [Re: happihourhero]
    #14253335 - 04/07/11 02:05 PM (12 years, 9 months ago)



Extras: Filter Print Post Top
Offlineaquilon
Stranger

Registered: 04/07/11
Posts: 12
Last seen: 12 years, 9 months
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14255345 - 04/07/11 08:12 PM (12 years, 9 months ago)

i've read plenty of teks, thank you. i have specific questions still unanswered.


Extras: Filter Print Post Top
Offlinehappihourhero
Waitin..
Male

Registered: 02/18/11
Posts: 115
Last seen: 11 years, 7 months
Re: LC First-Timer, Procedure Rough-Draft [Re: aquilon]
    #14255378 - 04/07/11 08:18 PM (12 years, 9 months ago)

Ditch all of your silly ideas, and follow the Damn teks. They w3re designed by people with a lot more experience and know-how.


Extras: Filter Print Post Top
Offlinemrtoad25
groovy
 User Gallery

Registered: 02/28/11
Posts: 98
Last seen: 1 year, 11 months
Re: LC First-Timer, Procedure Rough-Draft [Re: aquilon]
    #14255395 - 04/07/11 08:21 PM (12 years, 9 months ago)

Quote:

aquilon said:
i've read plenty of teks, thank you. i have specific questions still unanswered.



What if you experiment and find what works best for you. I do it all the time.


Extras: Filter Print Post Top
Offlinesteelmonkey
Homejigger
Male

Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada Flag
Last seen: 2 years, 4 months
Re: LC First-Timer, Procedure Rough-Draft [Re: mrtoad25]
    #14255698 - 04/07/11 09:12 PM (12 years, 9 months ago)

I'd skip the whole blender idea and just pop a couple of marbles in the LC jars plus i should think the only way to try and hybridise would be using agar


--------------------


Extras: Filter Print Post Top
Offlineaquilon
Stranger
Registered: 04/07/11
Posts: 12
Last seen: 12 years, 9 months
Re: LC First-Timer, Procedure Rough-Draft [Re: steelmonkey]
    #14258474 - 04/08/11 12:57 PM (12 years, 9 months ago)

Yeah I won't be hybridizing, baby steps for me.

Ok, I've heard of people putting marbles in the jar. Are you sure it's violent enough? I'm just a little nervous that the myc will clog the syringe needle when I plunge it into grain bags. I've only seen LC once in person, snot blobs, alien baby goo, i thought it needed to be thoroughly shredded in a blender before it could make its way through a needle.


Extras: Filter Print Post Top
Offlinesteelmonkey
Homejigger
Male

Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada Flag
Last seen: 2 years, 4 months
Re: LC First-Timer, Procedure Rough-Draft [Re: aquilon]
    #14259386 - 04/08/11 04:02 PM (12 years, 9 months ago)

I actually put two small marbles in my LC's the trick is not to let it grow too thick between shakes or yes it will clog up a 18 guage needle.I'll usually give mine a shake after its the size of a marshmellow then depending on the growth rate i'll shake every second day.Each time you break up the mycelium it creates new growth points but you want to give it time to recooperate between shakings hope this helps


--------------------


Extras: Filter Print Post Top
Offlinesteelmonkey
Homejigger
Male

Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada Flag
Last seen: 2 years, 4 months
Re: LC First-Timer, Procedure Rough-Draft [Re: aquilon]
    #14259658 - 04/08/11 04:54 PM (12 years, 9 months ago)

This LC on its first shake yesterday and i'll shake it again tomorrow as it looks now you could still suck it into a syringe with a 18 guage needle.








--------------------


Extras: Filter Print Post Top
Offlineaquilon
Stranger
Registered: 04/07/11
Posts: 12
Last seen: 12 years, 9 months
Re: LC First-Timer, Procedure Rough-Draft [Re: steelmonkey]
    #14259793 - 04/08/11 05:25 PM (12 years, 9 months ago)

very helpful info steelmonkey, thx!

do you have to take care that you suck from the cloud, and not the clear part of the LC?


Extras: Filter Print Post Top
Offlinesteelmonkey
Homejigger
Male

Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada Flag
Last seen: 2 years, 4 months
Re: LC First-Timer, Procedure Rough-Draft [Re: aquilon]
    #14260143 - 04/08/11 06:35 PM (12 years, 9 months ago)

I try to draw up mycelium from the cloud but you'll notice after shaking there will be thin wisps of mycelium floating plus when you shake your basically spreading mycelium you can't see into the clear part of the LC


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Grow Bags   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* LC Question djalexr 459 3 02/06/06 02:00 PM
by djalexr
* Old Timer's method Dvoid 3,543 12 05/09/01 09:33 PM
by Hippie3
* need old timers straw tek link NiGGy 1,261 2 01/18/03 04:52 PM
by shroominbloom
* Been a while since serious post... QUESTION LC Anything4Poppies 568 3 06/25/05 03:02 PM
by Anything4Poppies
* Question for first timer! Salemz_Lot 1,037 13 10/03/05 07:13 AM
by willmafingerdo
* anyone ever use the 9er tec w/ the blender?? willmafingerdo 723 4 10/16/05 08:06 AM
by willmafingerdo
* first timer? kiss_the_sky03 485 6 08/26/05 02:32 PM
by scatmanrav
* lc to poo? lc to rye? X24 975 13 09/07/05 11:59 PM
by deanofmean

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,701 topic views. 16 members, 172 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.009 seconds on 14 queries.