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Amazon Shop: ½ Pint Jars, Rye Grain, Wild Bird Seed

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OfflineEspresso
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Registered: 03/31/03
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Analysis of colonization problem
    #1424736 - 04/03/03 12:41 AM (15 years, 24 days ago)

That damn amphibian just won't leave me alone. This time, he asked me for advice on why his jars had neither colonized nor become visibly contaminated after 14+ days. Since I don't know the first thing about mycology, I thought I might refer the problem to you and pass the info on to him (lousy amphibians, mooching the internet and refusing to drop any dough). Could his jars not have had enough water? Or did he kill the spores by flame sterilizing the needle? oR any other suggestions so he wouldn't repeat the mistake?


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Invisiblejtseaweed
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Re: Analysis of colonization problem [Re: Espresso]
    #1424765 - 04/03/03 12:49 AM (15 years, 24 days ago)

did he cool the needle, like wiping it down with alcohol or something to that effect?


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InvisibleShroomismM
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Re: Analysis of colonization problem [Re: Espresso]
    #1424768 - 04/03/03 12:50 AM (15 years, 24 days ago)

Substrate was most likely too hot during innculation, causing death



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Invisiblejtseaweed
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Re: Analysis of colonization problem [Re: Shroomism]
    #1424819 - 04/03/03 12:59 AM (15 years, 24 days ago)

yeah that too. how long did you let the jars cool in the pc or pot?


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InvisibleSixTango
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Re: Analysis of colonization problem [Re: Espresso]
    #1424854 - 04/03/03 01:13 AM (15 years, 24 days ago)

Whatz in da jars.......PF cakes, rye, wbs?

6T


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OfflineAngry Mycologist
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Re: Analysis of colonization problem [Re: Shroomism]
    #1424908 - 04/03/03 01:31 AM (15 years, 24 days ago)

Quote:

Substrate was most likely too hot during innculation, causing death



We don't have enough information to validate this.

What type of recipe did you use? What type of sterilization method?


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflineEspresso
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Re: Analysis of colonization problem [Re: Angry Mycologist]
    #1424927 - 04/03/03 01:38 AM (15 years, 24 days ago)

Standard MMGG substrate, pint jars, he let the needle cool for about 10-15 seconds after sterilization, and no alcohol was used to help sterilize.


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Invisiblejtseaweed
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Re: Analysis of colonization problem [Re: Espresso]
    #1424953 - 04/03/03 01:48 AM (15 years, 24 days ago)

your needle may still have been to hot so the spores died when going through the needle. Thats what i think. how about everyone else.


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InvisibleShroomismM
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Re: Analysis of colonization problem [Re: jtseaweed]
    #1424972 - 04/03/03 01:59 AM (15 years, 24 days ago)

I've innoculated jars with a hot ass needle with no problem..the first drops of water cool down the needle. So I doubt that was the problem. I'm not sure that too hot jars was the problem but that's my best guess based on the information given.


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Invisiblegeorge castanzaM
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Re: Analysis of colonization problem [Re: Shroomism]
    #1425075 - 04/03/03 02:37 AM (15 years, 24 days ago)

bunk syringe?


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InvisibleShroomismM
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Re: Analysis of colonization problem [Re: george castanza]
    #1425165 - 04/03/03 03:08 AM (15 years, 24 days ago)

Ya..either bunk syringe.. jars were too hot.. or something else I can't think of.. how long did you let the jars cool after you cooked them?


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OfflineEspresso
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Re: Analysis of colonization problem [Re: Shroomism]
    #1425818 - 04/03/03 12:26 PM (15 years, 24 days ago)

jars cooled overnight after cooking, so i doubt that was it.


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Amazon Shop: ½ Pint Jars, Rye Grain, Wild Bird Seed

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