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Amazon Shop for: ½ Pint Jars, Rye Grain, Wild Bird Seed

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OfflineEspresso
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Registered: 03/31/03
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Analysis of colonization problem
    #1424736 - 04/03/03 12:41 AM (13 years, 8 months ago)

That damn amphibian just won't leave me alone. This time, he asked me for advice on why his jars had neither colonized nor become visibly contaminated after 14+ days. Since I don't know the first thing about mycology, I thought I might refer the problem to you and pass the info on to him (lousy amphibians, mooching the internet and refusing to drop any dough). Could his jars not have had enough water? Or did he kill the spores by flame sterilizing the needle? oR any other suggestions so he wouldn't repeat the mistake?


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Invisiblejtseaweed
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Re: Analysis of colonization problem [Re: Espresso]
    #1424765 - 04/03/03 12:49 AM (13 years, 8 months ago)

did he cool the needle, like wiping it down with alcohol or something to that effect?


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InvisibleShroomismM
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Re: Analysis of colonization problem [Re: Espresso]
    #1424768 - 04/03/03 12:50 AM (13 years, 8 months ago)

Substrate was most likely too hot during innculation, causing death



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Invisiblejtseaweed
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Re: Analysis of colonization problem [Re: Shroomism]
    #1424819 - 04/03/03 12:59 AM (13 years, 8 months ago)

yeah that too. how long did you let the jars cool in the pc or pot?


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InvisibleSixTango
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Re: Analysis of colonization problem [Re: Espresso]
    #1424854 - 04/03/03 01:13 AM (13 years, 8 months ago)

Whatz in da jars.......PF cakes, rye, wbs?

6T


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OfflineAngry Mycologist
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Re: Analysis of colonization problem [Re: Shroomism]
    #1424908 - 04/03/03 01:31 AM (13 years, 8 months ago)

Quote:

Substrate was most likely too hot during innculation, causing death



We don't have enough information to validate this.

What type of recipe did you use? What type of sterilization method?


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflineEspresso
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Re: Analysis of colonization problem [Re: Angry Mycologist]
    #1424927 - 04/03/03 01:38 AM (13 years, 8 months ago)

Standard MMGG substrate, pint jars, he let the needle cool for about 10-15 seconds after sterilization, and no alcohol was used to help sterilize.


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Invisiblejtseaweed
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Re: Analysis of colonization problem [Re: Espresso]
    #1424953 - 04/03/03 01:48 AM (13 years, 8 months ago)

your needle may still have been to hot so the spores died when going through the needle. Thats what i think. how about everyone else.


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InvisibleShroomismM
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Re: Analysis of colonization problem [Re: jtseaweed]
    #1424972 - 04/03/03 01:59 AM (13 years, 8 months ago)

I've innoculated jars with a hot ass needle with no problem..the first drops of water cool down the needle. So I doubt that was the problem. I'm not sure that too hot jars was the problem but that's my best guess based on the information given.


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Invisiblegeorge castanzaM
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Re: Analysis of colonization problem [Re: Shroomism]
    #1425075 - 04/03/03 02:37 AM (13 years, 8 months ago)

bunk syringe?


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pftek said:Impossible to clean everything right now. Maybe in a week.





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InvisibleShroomismM
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Re: Analysis of colonization problem [Re: george castanza]
    #1425165 - 04/03/03 03:08 AM (13 years, 8 months ago)

Ya..either bunk syringe.. jars were too hot.. or something else I can't think of.. how long did you let the jars cool after you cooked them?


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OfflineEspresso
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Re: Analysis of colonization problem [Re: Shroomism]
    #1425818 - 04/03/03 12:26 PM (13 years, 8 months ago)

jars cooled overnight after cooking, so i doubt that was it.


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Amazon Shop for: ½ Pint Jars, Rye Grain, Wild Bird Seed

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