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Offlinefires21
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Registered: 04/06/11
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Last seen: 3 years, 5 months
am i doing this right? mushroom cultivation from agar petri dish to pf tek (i think) *DELETED*
    #14245817 - 04/06/11 01:40 AM (12 years, 9 months ago)

Post deleted by fires21

Reason for deletion: privacy


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Invisiblelucas_southoz
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Registered: 06/09/10
Posts: 1,196
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Re: am i doing this right? mushroom cultivation from agar petri dish to pf tek (i think) [Re: fires21]
    #14245833 - 04/06/11 01:47 AM (12 years, 9 months ago)

I would throw them out.

If you want you can keep them and see if it is contaminated but i don't the chances of it being clean.


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Invisiblewildernessjunkie
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Registered: 06/13/10
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Re: am i doing this right? mushroom cultivation from agar petri dish to pf tek (i think) [Re: lucas_southoz]
    #14245920 - 04/06/11 02:17 AM (12 years, 9 months ago)

Why couldnt you get spores?


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Invisiblefngbronco
Monkey Man
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Registered: 09/26/10
Posts: 2,877
Re: am i doing this right? mushroom cultivation from agar petri dish to pf tek (i think) [Re: wildernessjunkie]
    #14245938 - 04/06/11 02:24 AM (12 years, 9 months ago)

Sounds like you transferred cobweb mold to cakes (which probably contaminated when opened). Agar works best when transferred to grains.


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I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek


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Offlinefires21
Stranger
Registered: 04/06/11
Posts: 11
Last seen: 3 years, 5 months
1 [Re: wildernessjunkie]
    #14248035 - 04/06/11 03:22 PM (12 years, 9 months ago)

1


Edited by fires21 (08/13/20 09:01 AM)


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InvisibleFischer
Teonanacatalyst
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Registered: 06/03/10
Posts: 565
Loc: Downunder
Re: am i doing this right? mushroom cultivation from agar petri dish to pf tek (i think) [Re: fires21]
    #14248078 - 04/06/11 03:31 PM (12 years, 9 months ago)

I didn't think you could clone dried shrooms to agar; that they are only useful when fresh, and best used within 2 days of picking?


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Invisiblefngbronco
Monkey Man
Male


Registered: 09/26/10
Posts: 2,877
Re: am i doing this right? mushroom cultivation from agar petri dish to pf tek (i think) [Re: Fischer]
    #14248575 - 04/06/11 04:49 PM (12 years, 9 months ago)

You can tell their contaminated before the myc starts to grow. Growing from dried tissue is possible but from what I've gathered, unless you know what you're doing, it's highly improbable.


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek


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Offlineafrosheen
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Registered: 03/06/10
Posts: 1,878
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Re: am i doing this right? mushroom cultivation from agar petri dish to pf tek (i think) [Re: fngbronco]
    #14248721 - 04/06/11 05:18 PM (12 years, 9 months ago)

Growing from a dried cap is probably extremely possible, given that it'll be loaded with dried spores that just need hydration, but if you're gonna half ass it and not use sterile techniques, you will only fail.

Also if you did have success on agar, you'd do a glovebox transfer of healthy mycelium to a prepared liquid culture solution or to grains. Throwing a chunk of mycelium in a BRF jar probably won't work very well.


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Edited by afrosheen (04/06/11 05:19 PM)


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Offlinefires21
Stranger
Registered: 04/06/11
Posts: 11
Last seen: 3 years, 5 months
1 [Re: afrosheen]
    #14250766 - 04/06/11 11:38 PM (12 years, 9 months ago)

1


Edited by fires21 (08/13/20 09:02 AM)


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Invisiblefngbronco
Monkey Man
Male


Registered: 09/26/10
Posts: 2,877
Re: am i doing this right? mushroom cultivation from agar petri dish to pf tek (i think) [Re: fires21]
    #14251268 - 04/07/11 02:19 AM (12 years, 9 months ago)

Leave it be and post a picture of the growth when it shows on the sides.


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek


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