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OfflineBelief
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Registered: 03/14/11
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Antibiotic Agar. To diliute or not to dilute?
    #14159572 - 03/21/11 02:31 PM (12 years, 11 months ago)

So I usually dilute my regular commercial agar mix to about %50-%60 of the recommended density to promote faster growth when agar to grained

However when dealing with pre-mixed antibiotic agar from FP,  I follow the recommended density and still get some bacterial contamination.  It's not past it's expiration date. 

should I add more than the recommended 100% density? 
what kind of antibiotic is in the FP brand?
is there any way to make it more effective against bacteria? 

I am trying to clone some wild specimens.  I've isolated away from other molds/fungi but the moisture from the tissue is promoting bacterial growth.

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Offlinedoulovebeef
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Re: Antibiotic Agar. To diliute or not to dilute? [Re: Belief]
    #14244090 - 04/05/11 08:09 PM (12 years, 10 months ago)

have you tried pouring liquid agar over the contaminated plate? allow the myc to crawl through and transfer.

i know this doesnt answer any of your questions, sorry. but just a thought.

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Offlinepslyke
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Re: Antibiotic Agar. To diliute or not to dilute? [Re: doulovebeef]
    #14244192 - 04/05/11 08:26 PM (12 years, 10 months ago)

Quote:

doulovebeef said:
have you tried pouring liquid agar over the contaminated plate? allow the myc to crawl through and transfer.

i know this doesnt answer any of your questions, sorry. but just a thought.




If I could just add---if you use this approach, make sure that the liquid agar has cooled sufficiently or it will kill everything on the plate. It is a delicate balance between keeping the agar hot enough to poor and cool enough that it hasn't solidified.

With regards to antibiotics suspended in agar--they tend to be quite labile and many of them are also light sensitive. It is not at all unreasonable to think that they may have gone off.


--------------------
"What appears impenetrable to us does exist, manifesting itself in the deepest wisdom and the most radiant beauty" Einstein

"The conservatives of 70 years ago would be outraged at what has come to pass. It embodies everything they took up arms for to defeat"Asante


:kratom:

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